DADA2 pipeline
Alexis Carteron & Simon Morvan
- Introduction
- Commençons ! / Let’s start
- Profile de qualité / Quality profiles
- Filtrage et tronquage / Filtering and Trimming
- Apprentissage des taux d’erreur / Error Rates Learning
- Déreplication / Dereplication
- Tableau des ASV / ASV table
- seqtab.nochim.nosd = seqtab.nochim[,colSums(seqtab.nochim) > 200]
{r assign_taxo, include=TRUE,cache=TRUE} #taxotab <- assignTaxonomy(seqtab.nochim, refFasta = "reference_database/sh_general_release_dynamic_01.12.2017.fasta", minBoot = 50, #Default 50. The minimum bootstrap confidence for assigning a taxonomic level. outputBootstraps = TRUE, multithread = TRUE) #- save(taxtab, file=“data/taxotab.01.05.rdata”)
- NB1: dada2 does not throw away singleton reads.
- However, it does not infer biological sequence variants that are only supported by a single read - singletons are assumed too difficult to differentiate from errors.
- Hence no singletons in the output table of amplicon sequence variants.
- NB2: DADA2 consistently measures diversity across different filtering parameters and error rates. OTU methods do not.
- https://twitter.com/bejcal/status/771010634074820608
- NB3: The ASVs with no species assignment do not match the same species in over 50% of the bootstrap replicate kmer-based assignments
- (see Wang et al. 2007 for more info on the naive Bayesian classifier method).
Date: 2018-05-03 10:03:26
Introduction
Ce pipeline consiste en une série d’étapes permettant d’obtenir des séquences analysables pour les études de communautés microbiennes. A l’origine construit pour les séquences 16S, nous l’utiliserons avec des séquences amplifiées de gène marqueur ITS (Fungi) obtenus grâce à un séquençage en paire Illumina MiSEQ 2x300 paires de bases.
Avant de débuter ce pipeline, il faut prendre quelques précautions:
- Les échantillons doivent être démultiplexés chaque échantillon doit avoir son propre fichier fastq.
- Les nucléotides qui ne font pas partie de l’amplicon (amorces, adaptateurs, bar-code) doivent avoir été retirées. Dans le cas contraire, ils devront être à l’étape de filtrage.
- En cas de séquençage en paire, les séquences sens et anti-sens doivent être dans deux fichier fastq distincts et être dans le même ordre dans les deux fichiers.
- La plupart des fonctions présentées ont une option de multithreading qui permet d’accélerer les temps de calcul en accédant à plusieurs processeurs. Il suffit d’indiquer multithread = TRUE pour l’activer. Attention, cette option ne marche pas sous Windows
This pipeline is designed in order to obtain analysable sequences for microbial community studies. Originally made for 16S sequences, we will use it here with amplified ITS sequences (Fungi) generated by Illumina MiSEQ 2x300 bp paired-end sequencing.
Before starting, we must take a few precautions:
- Samples must be demultiplexed: split into individual per-sample fastqs
- The nucleotides which are not part of the amplicon (primers, adapters, bar-code) have to be removed. They can be removed at the filtring step.
- If the sequences are paired-end, the forward and reverse sequences have to be in distinct fastq files but must contain reads in matched order.
- Most functions have a multithreading option that allows faster computing time by accessing multiple processors. Just specify multithread = TRUE to enable it. Warning, this option does not work under Windows.
Commençons ! / Let’s start
Nous allons tout d’abord chargé la librairie DADA2. Vous devriez avoir la denière version: 1.6.0.
Puis nous allons créer une variable indiquant le chemin qui permettra d’accéder aux objets dont nous allons avoir besoin.
First, we’re going to load the DADA2 package. You should have the latest version : 1.6.0. Then we’re going to create a variable indicating the path which will allow to access the objects required for this pipeline.
library(dada2); packageVersion("dada2")## Loading required package: Rcpp## [1] '1.6.0'path <- "data/ITS_sub/"Vérifions ce qu’il y a au bout du chemin / Let’s check where the path leads to
list.files(path)## [1] "S10_R1.fastq" "S10_R2.fastq" "S11_R1.fastq" "S11_R2.fastq"
## [5] "S12_R1.fastq" "S12_R2.fastq" "S13_R1.fastq" "S13_R2.fastq"
## [9] "S14_R1.fastq" "S14_R2.fastq" "S15_R1.fastq" "S15_R2.fastq"
## [13] "S16_R1.fastq" "S16_R2.fastq" "S17_R1.fastq" "S17_R2.fastq"
## [17] "S18_R1.fastq" "S18_R2.fastq" "S1_R1.fastq" "S1_R2.fastq"
## [21] "S20_R1.fastq" "S20_R2.fastq" "S21_R1.fastq" "S21_R2.fastq"
## [25] "S22_R1.fastq" "S22_R2.fastq" "S23_R1.fastq" "S23_R2.fastq"
## [29] "S24_R1.fastq" "S24_R2.fastq" "S25_R1.fastq" "S25_R2.fastq"
## [33] "S26_R1.fastq" "S26_R2.fastq" "S27_R1.fastq" "S27_R2.fastq"
## [37] "S28_R1.fastq" "S28_R2.fastq" "S29_R1.fastq" "S29_R2.fastq"
## [41] "S2_R1.fastq" "S2_R2.fastq" "S30_R1.fastq" "S30_R2.fastq"
## [45] "S31_R1.fastq" "S31_R2.fastq" "S33_R1.fastq" "S33_R2.fastq"
## [49] "S34_R1.fastq" "S34_R2.fastq" "S35_R1.fastq" "S35_R2.fastq"
## [53] "S36_R1.fastq" "S36_R2.fastq" "S37_R1.fastq" "S37_R2.fastq"
## [57] "S39_R1.fastq" "S39_R2.fastq" "S3_R1.fastq" "S3_R2.fastq"
## [61] "S41_R1.fastq" "S41_R2.fastq" "S42_R1.fastq" "S42_R2.fastq"
## [65] "S43_R1.fastq" "S43_R2.fastq" "S44_R1.fastq" "S44_R2.fastq"
## [69] "S45_R1.fastq" "S45_R2.fastq" "S46_R1.fastq" "S46_R2.fastq"
## [73] "S47_R1.fastq" "S47_R2.fastq" "S48_R1.fastq" "S48_R2.fastq"
## [77] "S49_R1.fastq" "S49_R2.fastq" "S4_R1.fastq" "S4_R2.fastq"
## [81] "S50_R1.fastq" "S50_R2.fastq" "S51_R1.fastq" "S51_R2.fastq"
## [85] "S52_R1.fastq" "S52_R2.fastq" "S53_R1.fastq" "S53_R2.fastq"
## [89] "S54_R1.fastq" "S54_R2.fastq" "S55_R1.fastq" "S55_R2.fastq"
## [93] "S56_R1.fastq" "S56_R2.fastq" "S58_R1.fastq" "S58_R2.fastq"
## [97] "S59_R1.fastq" "S59_R2.fastq" "S5_R1.fastq" "S5_R2.fastq"
## [101] "S60_R1.fastq" "S60_R2.fastq" "S61_R1.fastq" "S61_R2.fastq"
## [105] "S62_R1.fastq" "S62_R2.fastq" "S63_R1.fastq" "S63_R2.fastq"
## [109] "S64_R1.fastq" "S64_R2.fastq" "S65_R1.fastq" "S65_R2.fastq"
## [113] "S66_R1.fastq" "S66_R2.fastq" "S67_R1.fastq" "S67_R2.fastq"
## [117] "S68_R1.fastq" "S68_R2.fastq" "S69_R1.fastq" "S69_R2.fastq"
## [121] "S6_R1.fastq" "S6_R2.fastq" "S72_R1.fastq" "S72_R2.fastq"
## [125] "S73_R1.fastq" "S73_R2.fastq" "S74_R1.fastq" "S74_R2.fastq"
## [129] "S75_R1.fastq" "S75_R2.fastq" "S76_R1.fastq" "S76_R2.fastq"
## [133] "S77_R1.fastq" "S77_R2.fastq" "S78_R1.fastq" "S78_R2.fastq"
## [137] "S79_R1.fastq" "S79_R2.fastq" "S7_R1.fastq" "S7_R2.fastq"
## [141] "S80_R1.fastq" "S80_R2.fastq" "S81_R1.fastq" "S81_R2.fastq"
## [145] "S8_R1.fastq" "S8_R2.fastq" "S9_R1.fastq" "S9_R2.fastq"Vous devriez voir les noms des fichiers fastq.
You should see the names of the fastq files
Nous allons maintenant lire les noms des fichiers fastq, et manipuler leur chaine de charactères variables pour obtenir une liste des fastq sens et antisens. La fonction sort permet d’obtenir le même ordre entre les fastq sens et antisens.
Now, we’re goign to read in the names of the fastq files, and perform some string manipulation to get lists of the forward and reverse fastq files. The sort function ensures forward/reverse reads are in the same order.
fnFs <- sort(list.files(path, pattern="_R1.fastq"))
fnRs <- sort(list.files(path, pattern="_R2.fastq"))Etant donné que les paires de fichiers fastq sens et antisens appartiennent au même échantillon, nous allons créer une variable qui extrait le nom de cet échantillon. Dans ce cas, nous partons du principe que les noms des fichiers fastq ont un format: SAMPLENAME_XXX.fastq
Given that the forward/reverse fastq pairs belong to the same sample, we are going to extract the name and save it in a variable. In this case we assume that the filenames have this type of format: SAMPLENAME_XXX.fastq
sample.names <- sapply(strsplit(fnFs, "_"), `[`, 1)
sample.names ## [1] "S10" "S11" "S12" "S13" "S14" "S15" "S16" "S17" "S18" "S1" "S20"
## [12] "S21" "S22" "S23" "S24" "S25" "S26" "S27" "S28" "S29" "S2" "S30"
## [23] "S31" "S33" "S34" "S35" "S36" "S37" "S39" "S3" "S41" "S42" "S43"
## [34] "S44" "S45" "S46" "S47" "S48" "S49" "S4" "S50" "S51" "S52" "S53"
## [45] "S54" "S55" "S56" "S58" "S59" "S5" "S60" "S61" "S62" "S63" "S64"
## [56] "S65" "S66" "S67" "S68" "S69" "S6" "S72" "S73" "S74" "S75" "S76"
## [67] "S77" "S78" "S79" "S7" "S80" "S81" "S8" "S9"Specify the full path to the fnFs and fnRs
fnFs <- file.path(path, fnFs)
fnRs <- file.path(path, fnRs)Pour les plus maniaques d’entre nous, ce script permet d’ordonner les noms des échantillons. Pour les plus maniaques d’entre nous, ce script permet d’ordonner les noms des échantillons.
library(gtools)
sample.names <- mixedsort(sample.names)
fnFs <- mixedsort(fnFs)
fnRs <- mixedsort(fnRs)
sample.names## [1] "S1" "S2" "S3" "S4" "S5" "S6" "S7" "S8" "S9" "S10" "S11"
## [12] "S12" "S13" "S14" "S15" "S16" "S17" "S18" "S20" "S21" "S22" "S23"
## [23] "S24" "S25" "S26" "S27" "S28" "S29" "S30" "S31" "S33" "S34" "S35"
## [34] "S36" "S37" "S39" "S41" "S42" "S43" "S44" "S45" "S46" "S47" "S48"
## [45] "S49" "S50" "S51" "S52" "S53" "S54" "S55" "S56" "S58" "S59" "S60"
## [56] "S61" "S62" "S63" "S64" "S65" "S66" "S67" "S68" "S69" "S72" "S73"
## [67] "S74" "S75" "S76" "S77" "S78" "S79" "S80" "S81"
Profile de qualité / Quality profiles
Cette première étape permet de visualiser la qualité des séquences grâce au Q score associé à chaque nucléotide.
This first step allows to visualize the sequences quality thanks to the individual Q score of each nucleotide
plotQualityProfile(fnFs[1])
plotQualityProfile(fnRs[1]) 
Dans ces figures, la médianne est en vert, et les quartiles en orange pointillé. Ici, nous avons choisi de visualiser le premier échantillon sens (fnFs[1]) et antisens (fnRs[1]), mais il est possible de visualiser plusieurs graphiques en même temps (fnFs[x:y]) ou les aggréger comme ce qui suit.
In these figures, the median is in green and the quartiles are the dotted orange lines. Here we only plotted the first forward and reverse fastq (fnFs[1] and fnRs[1]), but it is possible to plot multiple figures(fnFs[x:y]) or aggregate them as follows.
plotQualityProfile(fnFs, aggregate = TRUE)
plotQualityProfile(fnRs, aggregate = TRUE)
L’analyse de ces graphiques nous permet de choisir les paramètres de filtrage et de rognage de la prochaine étape. En effet, l’indice de Q score nous renseigne sur la précision du séquençage (voir tableau ci-dessous).
The analysis of these figures helps to choose the filtring and trimming parameters of the next step. The Q score index gives us information on sequencing’s accuracy (see table).
| Q score | Precision |
|---|---|
| 10 | 90 % |
| 20 | 99 % |
| 30 | 99.9 % |
| 40 | 99.99 % |
Filtrage et tronquage / Filtering and Trimming
Tout d’abord nous allons créer un dossier (filtered_pairedend) et des objets (filtFs et filtRs) pour stoquer les séquences filtrées.
First we will create a directoy (filtered_pairedend) and objects (filtFs and filtRs) to store the filtered sequences.
filt_path <- file.path(path, "filtered_pairedend")
filtFs <- file.path(filt_path, paste0(sample.names, "_F_filt.fastq.gz"))
filtRs <- file.path(filt_path, paste0(sample.names, "_R_filt.fastq.gz"))Procédons avec la fonction filterAndTrim, sa sortie sera stocké dans l’objet out.
Let’s procede with the filterAndTrim function, its output will be stored in the out object
out <- filterAndTrim(fnFs, filtFs, fnRs, filtRs,
truncQ=6,
truncLen = c(280,280),
trimLeft=c(18,20),
maxEE=c(2,2))## Creating output directory: data/ITS_sub//filtered_pairedendEn premier lieu, la fonction a besoin des séquences non filtrées (fnFs et FnRs) ainsi que les noms des objets des séquences filtrées (filtFs et filtRs). Plusieurs paramètres peuvent ensuite être modifiés à notre guise:
- truncQ : définit un l’indice Q score minimale. A la première instance d’un score de qualité inférieur ou égal à truncQ, la séquence est tronquée.
- truncLen : définit à quelle longueur les séquences vont être tronquées. Les séquences plus courtes que la longueur sont éliminées.
- trimLeft : définit la longueur que l’on coupe du côté 5’ des séquences. Celà permet d’enlever les amorces si ça n’a pas été fait préalablement.
- maxEE : définit le nombre maximum d ’“erreurs attendues” autorisées dans une lecture. Ce filtre se base sur l’indice Q score. Plus on augmente le chiffre moins on est strict.
D’autres paramètres peuvent également être modifiés, ils sont accessibles à la page d’aide de la fonction: ?filterAndTrim
Amorces / Primers ITS3_KYO2: GATGAAGAACGYAGYRAA = 18bp ITS4: TCCTCCGCTTATTGATATGC = 20b
First, the function needs the unfiltered sequences (fnFs and FnRs) as well as the names of the objects of the filtered sequences (filtFs and filtRs). Several parameters can then be modified as we wish:
- truncQ: sets a minimum Q score. At the first instance of a quality score less than or equal to truncQ, the sequence is truncated.
- truncLen: sets the length at which the sequences will be truncated. Sequences shorter than the length are eliminated.
- trimLeft: sets the length that will be removed on the 5’ side of the reads. This allows you to remove the primers if it has not been done beforehand.
- maxEE: sets the maximum number of “expected errors” allowed in a read. This filter is based on the Q index. The more the number is increased, the less strict we are.
Other settings can also be changed, they are accessible on the help page of the function : ?FilterAndTrim
Visualisation du filtrage / Filtring visualization
pourc <- cbind((out[,2]/out[,1])*100) # Percentage filtered sequence / non-filtered sequence
pourc_disc <- cbind(out, pourc) # combines out and pourc
pourc_disc ## reads.in reads.out
## S1_R1.fastq 1000 595 59.5
## S2_R1.fastq 1000 482 48.2
## S3_R1.fastq 1000 556 55.6
## S4_R1.fastq 1000 425 42.5
## S5_R1.fastq 1000 387 38.7
## S6_R1.fastq 1000 619 61.9
## S7_R1.fastq 1000 521 52.1
## S8_R1.fastq 1000 423 42.3
## S9_R1.fastq 1000 492 49.2
## S10_R1.fastq 1000 366 36.6
## S11_R1.fastq 1000 432 43.2
## S12_R1.fastq 1000 546 54.6
## S13_R1.fastq 1000 417 41.7
## S14_R1.fastq 1000 229 22.9
## S15_R1.fastq 1000 512 51.2
## S16_R1.fastq 1000 481 48.1
## S17_R1.fastq 1000 634 63.4
## S18_R1.fastq 1000 607 60.7
## S20_R1.fastq 1000 614 61.4
## S21_R1.fastq 1000 598 59.8
## S22_R1.fastq 1000 539 53.9
## S23_R1.fastq 1000 561 56.1
## S24_R1.fastq 1000 599 59.9
## S25_R1.fastq 1000 598 59.8
## S26_R1.fastq 1000 567 56.7
## S27_R1.fastq 1000 650 65.0
## S28_R1.fastq 1000 607 60.7
## S29_R1.fastq 1000 526 52.6
## S30_R1.fastq 1000 551 55.1
## S31_R1.fastq 1000 571 57.1
## S33_R1.fastq 1000 482 48.2
## S34_R1.fastq 1000 644 64.4
## S35_R1.fastq 1000 558 55.8
## S36_R1.fastq 1000 453 45.3
## S37_R1.fastq 1000 446 44.6
## S39_R1.fastq 1000 521 52.1
## S41_R1.fastq 1000 517 51.7
## S42_R1.fastq 1000 447 44.7
## S43_R1.fastq 1000 500 50.0
## S44_R1.fastq 1000 369 36.9
## S45_R1.fastq 1000 359 35.9
## S46_R1.fastq 1000 540 54.0
## S47_R1.fastq 1000 501 50.1
## S48_R1.fastq 1000 391 39.1
## S49_R1.fastq 1000 516 51.6
## S50_R1.fastq 1000 422 42.2
## S51_R1.fastq 1000 567 56.7
## S52_R1.fastq 1000 521 52.1
## S53_R1.fastq 1000 599 59.9
## S54_R1.fastq 1000 472 47.2
## S55_R1.fastq 1000 571 57.1
## S56_R1.fastq 1000 416 41.6
## S58_R1.fastq 1000 525 52.5
## S59_R1.fastq 1000 559 55.9
## S60_R1.fastq 1000 593 59.3
## S61_R1.fastq 1000 537 53.7
## S62_R1.fastq 1000 470 47.0
## S63_R1.fastq 1000 644 64.4
## S64_R1.fastq 1000 595 59.5
## S65_R1.fastq 1000 561 56.1
## S66_R1.fastq 1000 551 55.1
## S67_R1.fastq 1000 544 54.4
## S68_R1.fastq 1000 532 53.2
## S69_R1.fastq 1000 372 37.2
## S72_R1.fastq 1000 558 55.8
## S73_R1.fastq 1000 581 58.1
## S74_R1.fastq 1000 431 43.1
## S75_R1.fastq 1000 356 35.6
## S76_R1.fastq 1000 403 40.3
## S77_R1.fastq 1000 387 38.7
## S78_R1.fastq 1000 400 40.0
## S79_R1.fastq 1000 560 56.0
## S80_R1.fastq 1000 457 45.7
## S81_R1.fastq 1000 594 59.4(mean(out[,2])/mean(out[,1]))*100 # Mean percentage ## [1] 50.98243Plus de la moitié des séquences n’ont pas passé nos paramètres de filtrage !
More than half the reads haven’t passed through the filtering parameters !
Apprentissage des taux d’erreur / Error Rates Learning
Cet étape consiste à estimer le taux d’erreur de séquençage. Son but est de permettre de différencier les séquences mutantes et les séquences érronées.Le modèle d’erreur est calculé en alternant l’estimation du taux d’erreur et l’inférence de la composition de l’échantillon
This step consist in estimating the error rates due to sequencing. Its purpose is to differentiate between mutant sequences and false sequences. The error model is computed by alternating estimation of the error rates and inference of sample composition until they converge on a jointly consistent solution
errF <- learnErrors(filtFs)## Initializing error rates to maximum possible estimate.
## Sample 1 - 595 reads in 364 unique sequences.
## Sample 2 - 482 reads in 294 unique sequences.
## Sample 3 - 556 reads in 363 unique sequences.
## Sample 4 - 425 reads in 278 unique sequences.
## Sample 5 - 387 reads in 280 unique sequences.
## Sample 6 - 619 reads in 348 unique sequences.
## Sample 7 - 521 reads in 328 unique sequences.
## Sample 8 - 423 reads in 221 unique sequences.
## Sample 9 - 492 reads in 317 unique sequences.
## Sample 10 - 366 reads in 238 unique sequences.
## Sample 11 - 432 reads in 298 unique sequences.
## Sample 12 - 546 reads in 344 unique sequences.
## Sample 13 - 417 reads in 324 unique sequences.
## Sample 14 - 229 reads in 162 unique sequences.
## Sample 15 - 512 reads in 319 unique sequences.
## Sample 16 - 481 reads in 341 unique sequences.
## Sample 17 - 634 reads in 378 unique sequences.
## Sample 18 - 607 reads in 344 unique sequences.
## Sample 19 - 614 reads in 365 unique sequences.
## Sample 20 - 598 reads in 387 unique sequences.
## Sample 21 - 539 reads in 345 unique sequences.
## Sample 22 - 561 reads in 344 unique sequences.
## Sample 23 - 599 reads in 384 unique sequences.
## Sample 24 - 598 reads in 394 unique sequences.
## Sample 25 - 567 reads in 316 unique sequences.
## Sample 26 - 650 reads in 294 unique sequences.
## Sample 27 - 607 reads in 339 unique sequences.
## Sample 28 - 526 reads in 365 unique sequences.
## Sample 29 - 551 reads in 337 unique sequences.
## Sample 30 - 571 reads in 375 unique sequences.
## Sample 31 - 482 reads in 287 unique sequences.
## Sample 32 - 644 reads in 388 unique sequences.
## Sample 33 - 558 reads in 303 unique sequences.
## Sample 34 - 453 reads in 277 unique sequences.
## Sample 35 - 446 reads in 318 unique sequences.
## Sample 36 - 521 reads in 364 unique sequences.
## Sample 37 - 517 reads in 322 unique sequences.
## Sample 38 - 447 reads in 327 unique sequences.
## Sample 39 - 500 reads in 326 unique sequences.
## Sample 40 - 369 reads in 275 unique sequences.
## Sample 41 - 359 reads in 251 unique sequences.
## Sample 42 - 540 reads in 372 unique sequences.
## Sample 43 - 501 reads in 289 unique sequences.
## Sample 44 - 391 reads in 277 unique sequences.
## Sample 45 - 516 reads in 323 unique sequences.
## Sample 46 - 422 reads in 282 unique sequences.
## Sample 47 - 567 reads in 379 unique sequences.
## Sample 48 - 521 reads in 351 unique sequences.
## Sample 49 - 599 reads in 371 unique sequences.
## Sample 50 - 472 reads in 315 unique sequences.
## Sample 51 - 571 reads in 386 unique sequences.
## Sample 52 - 416 reads in 272 unique sequences.
## Sample 53 - 525 reads in 323 unique sequences.
## Sample 54 - 559 reads in 272 unique sequences.
## Sample 55 - 593 reads in 370 unique sequences.
## Sample 56 - 537 reads in 357 unique sequences.
## Sample 57 - 470 reads in 282 unique sequences.
## Sample 58 - 644 reads in 391 unique sequences.
## Sample 59 - 595 reads in 370 unique sequences.
## Sample 60 - 561 reads in 350 unique sequences.
## Sample 61 - 551 reads in 373 unique sequences.
## Sample 62 - 544 reads in 343 unique sequences.
## Sample 63 - 532 reads in 338 unique sequences.
## Sample 64 - 372 reads in 232 unique sequences.
## Sample 65 - 558 reads in 369 unique sequences.
## Sample 66 - 581 reads in 347 unique sequences.
## Sample 67 - 431 reads in 327 unique sequences.
## Sample 68 - 356 reads in 230 unique sequences.
## Sample 69 - 403 reads in 277 unique sequences.
## Sample 70 - 387 reads in 281 unique sequences.
## Sample 71 - 400 reads in 275 unique sequences.
## Sample 72 - 560 reads in 373 unique sequences.
## Sample 73 - 457 reads in 331 unique sequences.
## Sample 74 - 594 reads in 362 unique sequences.
## selfConsist step 2
## selfConsist step 3
## selfConsist step 4
## Convergence after 4 rounds.
## Total reads used: 37727errR <- learnErrors(filtRs)## Initializing error rates to maximum possible estimate.
## Sample 1 - 595 reads in 340 unique sequences.
## Sample 2 - 482 reads in 280 unique sequences.
## Sample 3 - 556 reads in 296 unique sequences.
## Sample 4 - 425 reads in 312 unique sequences.
## Sample 5 - 387 reads in 252 unique sequences.
## Sample 6 - 619 reads in 320 unique sequences.
## Sample 7 - 521 reads in 299 unique sequences.
## Sample 8 - 423 reads in 247 unique sequences.
## Sample 9 - 492 reads in 299 unique sequences.
## Sample 10 - 366 reads in 273 unique sequences.
## Sample 11 - 432 reads in 284 unique sequences.
## Sample 12 - 546 reads in 325 unique sequences.
## Sample 13 - 417 reads in 329 unique sequences.
## Sample 14 - 229 reads in 170 unique sequences.
## Sample 15 - 512 reads in 318 unique sequences.
## Sample 16 - 481 reads in 353 unique sequences.
## Sample 17 - 634 reads in 364 unique sequences.
## Sample 18 - 607 reads in 286 unique sequences.
## Sample 19 - 614 reads in 332 unique sequences.
## Sample 20 - 598 reads in 338 unique sequences.
## Sample 21 - 539 reads in 317 unique sequences.
## Sample 22 - 561 reads in 310 unique sequences.
## Sample 23 - 599 reads in 327 unique sequences.
## Sample 24 - 598 reads in 373 unique sequences.
## Sample 25 - 567 reads in 288 unique sequences.
## Sample 26 - 650 reads in 243 unique sequences.
## Sample 27 - 607 reads in 310 unique sequences.
## Sample 28 - 526 reads in 336 unique sequences.
## Sample 29 - 551 reads in 308 unique sequences.
## Sample 30 - 571 reads in 351 unique sequences.
## Sample 31 - 482 reads in 276 unique sequences.
## Sample 32 - 644 reads in 343 unique sequences.
## Sample 33 - 558 reads in 283 unique sequences.
## Sample 34 - 453 reads in 278 unique sequences.
## Sample 35 - 446 reads in 313 unique sequences.
## Sample 36 - 521 reads in 313 unique sequences.
## Sample 37 - 517 reads in 309 unique sequences.
## Sample 38 - 447 reads in 311 unique sequences.
## Sample 39 - 500 reads in 332 unique sequences.
## Sample 40 - 369 reads in 272 unique sequences.
## Sample 41 - 359 reads in 251 unique sequences.
## Sample 42 - 540 reads in 346 unique sequences.
## Sample 43 - 501 reads in 292 unique sequences.
## Sample 44 - 391 reads in 278 unique sequences.
## Sample 45 - 516 reads in 331 unique sequences.
## Sample 46 - 422 reads in 301 unique sequences.
## Sample 47 - 567 reads in 340 unique sequences.
## Sample 48 - 521 reads in 348 unique sequences.
## Sample 49 - 599 reads in 354 unique sequences.
## Sample 50 - 472 reads in 330 unique sequences.
## Sample 51 - 571 reads in 335 unique sequences.
## Sample 52 - 416 reads in 259 unique sequences.
## Sample 53 - 525 reads in 306 unique sequences.
## Sample 54 - 559 reads in 272 unique sequences.
## Sample 55 - 593 reads in 321 unique sequences.
## Sample 56 - 537 reads in 329 unique sequences.
## Sample 57 - 470 reads in 276 unique sequences.
## Sample 58 - 644 reads in 343 unique sequences.
## Sample 59 - 595 reads in 328 unique sequences.
## Sample 60 - 561 reads in 342 unique sequences.
## Sample 61 - 551 reads in 352 unique sequences.
## Sample 62 - 544 reads in 324 unique sequences.
## Sample 63 - 532 reads in 295 unique sequences.
## Sample 64 - 372 reads in 221 unique sequences.
## Sample 65 - 558 reads in 341 unique sequences.
## Sample 66 - 581 reads in 330 unique sequences.
## Sample 67 - 431 reads in 331 unique sequences.
## Sample 68 - 356 reads in 246 unique sequences.
## Sample 69 - 403 reads in 297 unique sequences.
## Sample 70 - 387 reads in 273 unique sequences.
## Sample 71 - 400 reads in 295 unique sequences.
## Sample 72 - 560 reads in 353 unique sequences.
## Sample 73 - 457 reads in 329 unique sequences.
## Sample 74 - 594 reads in 347 unique sequences.
## selfConsist step 2
## selfConsist step 3
## selfConsist step 4
## Convergence after 4 rounds.
## Total reads used: 37727Le nombre minimum de séquences à utiliser pour l’apprentissage des taux d’erreur peut être précisé avec le paramètre nreads.
The minimum number of sequences to use for error rate learning can be specified with the nreads parameter.
Visualisation du taux d’erreur / Error rate visualization
plotErrors(errF, nominalQ=TRUE)## Warning: Transformation introduced infinite values in continuous y-axis
## Warning: Transformation introduced infinite values in continuous y-axis
plotErrors(errR, nominalQ=TRUE)## Warning: Transformation introduced infinite values in continuous y-axis
Les taux d’erreur pour chaque transition (A->C, A->G,…) sont affichés. Chaque point est un taux d’erreur observé pour chaque score de qualité consensuel. La ligne noire montre l’erreur après convergence. La ligne rouge montre l’erreur sous la définition nominale de la valeur Q (pour la technologie Illumina).
The error rates for each possible transition (eg. A->C, A->G, …) are shown. Points are the observed error rates for each consensus quality score. The black line shows the estimated error rates after convergence. The red line shows the error rates expected under the nominal definition of the Q-value (for Illumina technology).
The consensus quality profile of a unique sequence is the average of the positional qualities from the dereplicated reads.
Déreplication / Dereplication
Dans cette étape, toutes les séquences identiques vont être regroupées en séquences uniques auxquelles sont attribués des abondances. Cela va diminuer les temps de calcul subséquants en éliminant des comparaisons redondantes. Les séquences dérépliquées prennent le nom des échantillons d’où elles proviennent.
Combines all identical sequencing reads into into unique sequences with a corresponding abundance. It will reduce subsequent computation time by eliminating redundant comparisons. The dereplicated sequences take the name of the samples from which they come.
derepFs <- derepFastq(filtFs)
names(derepFs) <- sample.names
derepRs <- derepFastq(filtRs)
names(derepRs) <- sample.namesL’avantage de DADA2 réside dans la conservation d’un résumé des informations de qualité associées à chaque séquence unique. Le profil de qualité consensuel d’une séquence unique est la moyenne des qualités de position des lectures dérépliquées. Ces profils de qualité informent le modèle d’erreur de l’étape suivante d’inférence d’échantillon, ce qui augmente considérablement la précision de DADA2.
The advantage of DADA2 lies in the fact that it retains a summary of the quality information associated with each unique sequence. The consensus quality profile of a unique sequence is the average of the positional qualities from the dereplicated reads. These quality profiles inform the error model of the subsequent sample inference step, significantly increasing DADA2’s accuracy.
Inférence des échantillons / Sample Inference
dadaFs <- dada(derepFs,
err = errF,
pool=TRUE,
multithread=TRUE)## 74 samples were pooled: 37727 reads in 20364 unique sequences.dadaRs <- dada(derepRs,
err=errR,
pool=TRUE,
multithread=TRUE)## 74 samples were pooled: 37727 reads in 18908 unique sequences.dadaFs[[1]]## dada-class: object describing DADA2 denoising results
## 76 sample sequences were inferred from 364 input unique sequences.
## Key parameters: OMEGA_A = 1e-40, BAND_SIZE = 16, USE_QUALS = TRUEdadaRs[[1]]## dada-class: object describing DADA2 denoising results
## 77 sample sequences were inferred from 340 input unique sequences.
## Key parameters: OMEGA_A = 1e-40, BAND_SIZE = 16, USE_QUALS = TRUE#save(dadaRs, file="data/dadaRs.rdata")
#save(dadaFs, file="data/dadaFs.rdata")Fusion des séquences pairées / Merging paired reads
Tout l’intérêt du séquençage en paire réside dans le but de fusionner les deux brins afin d’accroitre notre confiance en leur fiabilité. La fusion permet également d’obtenir des amplicons plus long.
La fonction mergePairs nécessite qu’on lui fournisse les objets calculés dans les deux étapes précédentes (derep et dada). Puis, les paramètres que nous pouvons modifiés librement sont:
- minOverlap : définit la taille minimale du chevauchement des brins sens et anti-sens pour que leur fusion soit accéptée. Les séquences qui ne fusionnent pas sont éliminées.
- maxMismatch : définit le nombre maximal d’incompatibilité nucléotidique dans le chevauchement.
D’autres paramètres peuvent également être modifiés, ils sont accessibles à la page d’aide de la fonction: ?mergePairs. Par exemple, si returnRejects = TRUE, les paires qui ont été rejetées en raison de discordances dans la région de chevauchement sont conservés dans la sortie
The whole point of paired-end sequencing lies in the goal of merging the two strands to increase our confidence in their reliability. Merging also makes it possible to obtain longer amplicons.
The function mergePairs needs to be provided with the objects computed in the two preceding stages (derep and dada). Then, the parameters we can freely modify are:
- minOverlap: sets the minimum size of the overlap of the sense and antisense strands for their merge to be accepted. Sequences that are not merged are discarded.
- maxMismatch: sets the maximum number of nucleotidic incompatibilities in the overlap.
Other settings can also be changed, they are accessible on the help page of the function : ?mergePairs. For example, if returnRejects = TRUE, pairs that were rejected because of mismatches in the overlap region are kept in the output.
mergers <- mergePairs(dadaFs, derepFs, dadaRs, derepRs,
minOverlap = 12,
maxMismatch = 0)Examinons les résulats / Let’s inspect the results
head(mergers[[1]])## sequence
## 1 ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCACACCCGTTTGAGTGTCGTGAATACTCTCAACCTTCTTGGTTTCTTTGACCACGAAGGCTTGGACTTTGGAGGTTTTTCTTGCTGGCCTCTTTAGAAGCCAGCTCCTCCTAAATGAATGGGTGGGGTCCGCTTTGCTGATCCTCGACGTGATAAGCATCTCTTCTACGTCTCAGTGTCAGCTCGGAACCCCGCTTTCCAACCGTCTTTGGACAAAGACAATGTTCGAGTTGCGACTCGACCTTACAAACCTTGACCTCAAATCGGGTGAGACTACCCGCTGAACTTAA
## 2 ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATCCCGAGGGGCATGCCTGTTCGAGCGTCATTTCACCACTCAAGCCTGGCTTGGTGTTGGGCGACGTCCCCTTTTGGGGACGCGTCTCGAAACGCTCGGCGGCGTGGCACCGGCTTTAAGCGTAGCAGAATCTTTCGCTTTGAAAGTCGGGGCCCCGTCTGCCGGAAGACCTACTCGCAAGGTTGACCTCGGATCAGGCAGGGATACCCGCTGAACTTAA
## 3 ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATAACCACTCAAGCCCCGGCTTGGTCTTGGGGTTCGCGGTCCGCGGCCCTTAAACTCAGTGGCGGTGCCGTCTGGCTCTAAGCGCAGTAATTCTCTCGCTATAGTGTCTAGGTGGTTGCTTGCCATAATCCCCCAATTTTTTACGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## 4 ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACTCCTTGGTATTCCGAGGAGTATGCCTGTTTCAGTATCATGAGCACTCTCACACCTAACCTTTGGGTTTATGGCGTGGAATTGGAATGCGCCGACTGTCATGGTTGGCCCTTCTAAAATGTAGTTCTTGGCTGTCACCTAATACAGCAGTTTGGCCTAATAGTTTTGGCATTCATTGTCAAATCTTTGGCTAACATTTGCTCCAGGAGTCAGTCTTGATAATACAGAAAACTCATTCAAATTTTGATCTGAAATCAGGTAGGGCTACCCGCTGAACTTAA
## 5 ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATTGAATCTTTGAACGCATCTTGCGCCTCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTAGAACTATCAAAAAAATAGATGATTTCAATCGTTAATTTTTTTGGAATTGGAGGTGGTGCTGGTCTTTTTCCATTAATGGCCCAAGCTCCTCCGAAATGCATTAGCGAATGCAGTGCACTTTTTCTCCTTGCTTTTTCTGGGCATTGATAGTTTACTCTCATGCCCTAAGCTGGTAGGGAGGAAGTCACAGAATGCTTCCCGCTCCTGAATGTAATACAAAACTTGACGATCAAACCCCTCAAATCAGGCAGGACTACCCGCTGAACTTAA
## 6 ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATGATCAACCATCAAGCCTGGCTTGTCGTTGGACCCTGTTGTCTCTGGGCGACAGGTCCGAAAGATAATGACGGTGTCATGGCAACCCCGAATGCAACGAGCTTTTTTATAGGCACGCATTTAGTGGTTGGCAAGGCCCCCTCGTGCGTTATTATTTTCTTACGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## abundance forward reverse nmatch nmismatch nindel prefer accept
## 1 172 15 12 150 0 0 2 TRUE
## 2 43 8 9 230 0 0 1 TRUE
## 3 37 22 18 234 0 0 2 TRUE
## 4 34 3 2 179 0 0 2 TRUE
## 5 32 4 3 147 0 0 1 TRUE
## 6 21 12 10 218 0 0 1 TRUEmax(mergers[[1]]$nmatch) # Largest overlap ## [1] 257min(mergers[[1]]$nmatch) # Smallest overlap ## [1] 113Tableau des ASV / ASV table
ASV signifie variants de séquences d’amplicons. C’est le nom donné aux OTUs de DADA2. Cependant ils sont bien différents des OTUs car ils n’ont à aucun moment été regroupés (à part les séquences identiques).
ASV stands for amplicon sequence variant. It is the name given to DADA2’s OTUs. However, they are very different from OTUs because they have never been clustered together (except for identical sequences).
seqtab <- makeSequenceTable(mergers)## The sequences being tabled vary in length.head(seqtab)## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGTGTCATTAAATTATCAACCTTGCTCGCTTTTATTAGCTTGAGTTAGGCTTGGATGTGAGGGTTTTGCTGGCTTCCTTCAGTGGATGGTCTGCTCCCTTTGAATGCATTAGCGGGATCTCTTGTGGACCGTCACTTGGTGTGATAATTATCTATGCCTTGAGACTTTGAAACAAACTTATGAGAATCTGCTTATAACCGTCCTCACGGACAACTTTTGACAATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 3
## S2 5
## S3 2
## S4 2
## S5 2
## S6 1
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCGACGCCATTCGGCCGAGGGCACGTCTGCCTGGGTGTCACGCATCGTTGCCCCCAAACGCCTCCCGCCTCGCAAGGGGCGCGGGATCTCGTTTGGCGGCGGAAGTTGGCCTCCCGTGGGCCTGTGCTCGCGGTTAGCCTAAAAAGGAGTCCTCGGCGACGAGCGCCACGACAATCGGTGGTTGATGAGACCTCGGTCCCCGTCGTGCGTGTCTGGTCGCCACACGGTGTGACTCGTCGACCCTAACGCGTCGTACCCACGTCGCTCCCGACGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAA
## S1 2
## S2 0
## S3 0
## S4 9
## S5 0
## S6 1
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACTCCTTGGTATTCCGAGGAGTATGCCTGTTTCAGTATCATGAGCACTCTCACACCTAACCTTTGGGTTTATGGCGTGGAATTGGAATGCGCCGACTGTCATGGTTGGCCCTTCTAAAATGTAGTTCTTGGCTGTCACCTAATACAGCAGTTTGGCCTAATAGTTTTGGCATTCATTGTCAAATCTTTGGCTAACATTTGCTCCAGGAGTCAGTCTTGATAATACAGAAAACTCATTCAAATTTTGATCTGAAATCAGGTAGGGCTACCCGCTGAACTTAA
## S1 34
## S2 13
## S3 63
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATCCCGAGGGGCATGCCTGTTCGAGCGTCATTTCACCATTCAAGCCTGGCTTGGTGTTGGGCGACGTCCCTTTGGGGACGCGCTTCGAAACGCTCGGCGGCGTGGCACCGGCTTTAAGCGTAGCAGAATCTTTCGCTTTGAAAGTCGGGGCCCCGTCTGCCGGAAGACCTACTTCAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 4
## S2 0
## S3 0
## S4 5
## S5 0
## S6 19
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATTGAATCTTTGAACGCATCTTGCGCCTCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTAGAACTATCAAAAAAATAGATGATTTCAATCGTTAATTTTTTTGGAATTGGAGGTGGTGCTGGTCTTTTTCCATTAATGGCCCAAGCTCCTCCGAAATGCATTAGCGAATGCAGTGCACTTTTTCTCCTTGCTTTTTCTGGGCATTGATAGTTTACTCTCATGCCCTAAGCTGGTAGGGAGGAAGTCACAGAATGCTTCCCGCTCCTGAATGTAATACAAAACTTGACGATCAAACCCCTCAAATCAGGCAGGACTACCCGCTGAACTTAA
## S1 32
## S2 0
## S3 2
## S4 0
## S5 0
## S6 148
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCATTAGTATTCTATTGGGCATGCCTGTTCGAGCGTCATTTTGACCACTTAAGCCTAGCTTAGTGTTGGGAATCTACGTTTGTAGTTCCTTAAATCTAGTGGCGGAGCCGCAGTGGCCTCTGAGCGTAGTAATTTTTTCTCGCTTCTGAAAATACTGTAGTTTTTGCCATTAAACCCCCAATTTTTTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTGCAACCCTCAAGCTCTGCTTGGTATTGGGCTCCGTCTATTACTGACGGGCCTTAAAATCAGTGGCGGTGCCATTAGGCTCTAAGCGTAGTAATTTTTCTCGCTATGGACGACTAGTGGATACTTGCCAGTAACCCCTAATTTTTAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## GCGCGATAGGTATTGTGAATTGCAGAATTGTGAATCATCGAATTTTTGAACGCACATTGCACCCATTGGTATTCCGATGGGTATACTTGTTTGAGCGTCATTTCATTCTCCTTTTTGGGGTTTTAGCTTGAATAAATCTGGCTGAATTATAGTGGTGTGGCTACCAGACTACAACGTGATAGATATTTCGTTGGATGTGACTGGGATTGCTCACCTTAAAACAATTGTATAGACCTCAAATCAAGCAGGACTACCCGCTGAACTTAA
## S1 1
## S2 0
## S3 0
## S4 0
## S5 0
## S6 23
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCTTTGGTATTCCGAAGAGCATGCCTGTTTGAGTGTCATGAAAATATCAACCTTGACTTGGGTTTAGTGCTCTTGTCTTGGCTTGGATTTGGCTGTTTGCCGCTCGAAAGAGTCGGCTCAGCTTAAAAGTATTAGCTGGATCTGTCTTTGAGACTTGGTTTGACTTGGCGTAATAAGTTATTTCGCTGAGGACAATCTTCGGATTGGCCGAGTTTCTGGGACGTTTGTCCGCTTTCTAATACAAGTTCTAGCTTGCTAGACATGACTTTTTTATTATCTGGCCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 4
## S2 0
## S3 26
## S4 0
## S5 0
## S6 51
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCGTTAGGCCGAGGGCACGCCTGCCTGGGTGTCACGCATCGTTGCCCCCCCCCAAACCACCCCTCCCCTCTCGAAAGAGAGAGACGAGGGGACTTGGCCGTGGGCGGATATTGGCCTCCCGTGTGCCGAACGGCCCGCGGTTGGCCTAAATACGAGTTGTCGTCGGCGATGGACGTCGTGGCGTTCGGTGGTCAAATAAACCTCGAGCTCCCGTCGCGCGTACGTCGTCGGCCTAATAAGGCTCACCGACCCTGAAGCGTTGTGAACAGCGCACGCATCGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAA
## S1 0
## S2 7
## S3 3
## S4 7
## S5 30
## S6 4
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTGAATTCTCAAAAACCCTTGAACCGCGAGGTTTCAGGTTTTTGGTTATGGAGAGTGCCGGCCTTCAACCGAGTTCGGCTCCTCTGAAAAGCATTAGCAAAACCCTCTCCCCGATGGCTTTGTGGCATTGATAAGACTGTCCATGCCTTGCCCTTCTGCGGAAAGCGGTTGCTTCAACAATCGTCTGAAGTGACAGACAATTTTTTTTTCTTGACCATTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 154
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTATAACCAATCCAGCTCGCTGGGTCTTGGGCACCGCCGGCTGGCGGGCCCCAAAAGCAGTGGCGGTACGACCGGGCTCTCAGCGTAGTAATACTCCTCGCTACAGATCCCCGGGCGGCACCTGCCAGAACCCCCCATTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCACGCCTAGCGTGGTATTGGGCTCGCCCTGTAGGGCTTGCCTCAAAGTCAGTGGCGGCACCGTTTGACCCCTAAGCGTAGTAATACTCGTCGCTTCTGGTGTTGTGCGGAGGCCTGCCAGCAACCCCCATTTTTTAAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATCCCGAGGGGCATGCCTGTTCGAGCGTCATTTCACCACTCAAGCCTGGCTTGGTGTTGGGCGACGTCCCCTTTTGGGGACGCGTCTCGAAACGCTCGGCGGCGTGGCACCGGCTTTAAGCGTAGCAGAATCTTTCGCTTTGAAAGTCGGGGCCCCGTCTGCCGGAAGACCTACTCGCAAGGTTGACCTCGGATCAGGCAGGGATACCCGCTGAACTTAA
## S1 43
## S2 0
## S3 0
## S4 1
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATAACCAATCCAGCTCGCTGGGTCTTGGGCACCGCCAGTTGGTGGGCCTTAAAAGCAGTGGCGGTACGGCCGGGCTCTAAGCGTAGTAATACTCCTCGCTACAGACCTCCGGGCGGCACCTGCCAGAACCCCCCATTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTACGCTTGGTCTTGGGCCCTGCCGGCAACGGCTGGCCTTAAAATTAGTGGCGGTGCCATTGAGCTCTACGCGTAGTAATAAAACCCTCGCTTCTGAGTCTCGGTGGACGCTTGCCAGCAACCCCCAATTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCGACGCCATTCGGCCGAGGGCACGTCTGCCTGGGTGTCACGCATCGTTGCCCCCAAACGCCCCCGCCTCGCAAGGGGCGCGGGATCTCGTTTGGTGGCGGAAGTTGGCCTCCCGTGGGCCTGTGCTCGCGGTTAGCCTAAAAAAGAGTCCTCGGCGACGAGCGCCACGACAATCGGTGGTTGATGAGACCTCGGTCCCCGTCGTGCGTGTCTGGTCGCCACACGGTGTGACTCGTCGACCCTAACGCGTCGTACCCACGTCGCTCCCGACGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAA
## S1 2
## S2 0
## S3 0
## S4 19
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACTCCTTGGTATTCCGAGGAGTATGCCTGTTTCAGTATCATGAGCACTCTCACAACTAATCTTTGTTGGTTAAGATTGTGGAATTGGGATGCGCCAATTGTTATTGATTGGCCCTCCTAAAATGTAGCTCTTGACTGTGGACTACTACAGTAGTTTGGCCTAATAGTTTTGGCAGTTACATGTCAAATCTTTGGCTACATTTACTTTGGTCCATGCAGTCTTGATATACAGAAAACTCTAAATACAACTTTGATCTGAAATCAGGTAGGGCTACCCGCTGAACTTAA
## S1 2
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATGAAATCTTCAACCTATAAGCTTTTGTGGTTTGTAGGCTTGGACTTGGAGGCTTGTCGGCCTTGATCGGTCGGCTCCTCTTAAATGCATTAGCTTGATTCCTTGCGGATCGGCTCTCGGTGTGATAATATCTACGCCGCGACCGTGAAGCGTTTGGCGAGCTTCTAACCGTCTCACTTGAGAGACAACTTTATGACCTCTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 1
## S3 0
## S4 0
## S5 4
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCACACCTGTTCGAGCGCCATTTCTACCCTAGAGCCCTGCTCTGTGATGGGCCCCGTCCTCGCGGACGGGCCCGAAACCCGTGGGCGCCGTCGTCCGGCCCTGAGCGTAGCAAGAGAAATCCCTCGCTCGGAGTGTCTGTCGGTGGCCGCCCCGAACCATTTTTACAAGGTTGGCCTCGGATCAGGTGGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGTGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCCTGGTCATTCCGGGGAGCATGCCTGTTTGAGTGTCATAATACTCTCAACCCCTGGGGTCTTTAAGCGGACCACCTCGGGCTTGGATCATGGACGCTGCCGGCCCTCTCGGCGGTGTGGCTCGTCTGAAATACATGAGCTGACCTGTCTGCGAACAGCACGGTTTGACTCGGCGTAATAAATCCATTTTTCGTCGGGGACACCCTTTGCGGGGTGGCCGGGCTCGCGGTCTTCGGGTTGCTTACAATCGAATAGTCCCATCTATTTTAGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 1
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCATTAGTATTCTATTGGGCATGCCTGTTCGAGCGTCATTTTGACCACTTAAGCCTGGCTTAGTGTTGGGAATCTACGTTTGTAGTTCCTTAAATCTAGTGGCGGAGCCGCAGTGGCCTCTGAGCGTAGTAATTTTTTCTCGCTTTGGAAGGTTCTGTAGTTTTTGCCATAAAACCCCCAATTTTTTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATGATCAACCATCAAGCCTGGCTTGTCGTTGGACCCTGTTGTCTCTGGGCGACAGGTCCGAAAGATAATGACGGTGTCATGGCAACCCCGAATGCAACGAGCTTTTTTATAGGCACGCATTTAGTGGTTGGCAAGGCCCCCTCGTGCGTTATTATTTTCTTACGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 21
## S2 11
## S3 0
## S4 20
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATATTGCGCTCTTTGGTATTCCGAAGAGCATGCTTGTTTGAGTATCAGTAAACACCTCAACCTCCTCTTGTTTTTTCAAAAGGAGGGTGGACTTGAGCTATCCCAACAACCTTCACCGGTAGGCGGGCGGCTTGAAATGCAGGTGCAGCTGGACTTTTATCTGAGCTAAAAGCATATCTATTTAGTCCTCGTCAAACAGGATTATTACTATTGCTGCAGCTAACATAAAGGATAATTGTCCTCATTGCTGACTGATGCAGGATTTTACGACACTTTATGTGTTGTTCAACTCGATCTCAAATCAAGTAAGACTACCCGCTGAACTTAA
## S1 10
## S2 12
## S3 23
## S4 0
## S5 2
## S6 0
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCGACGCCATTCGGCCGAGGGCACGTCTGCCTGGGTGTCACGCATCGTTGCCCCCAAACGCCCCCGCCTCGCAAGGGGCGCGGGATCTCGTTTGGTGGCGGAAGTTGGCCTCCCGTGGGCCTGTGCTCGCGGTTAGCCTAAAAAGGAGTCCTCGGCGACGAGCGCCACGACAATCGGTGGTTGATGAGACCTCGGTCCCCGTCGTGCGTGTCTGGTCGCCACACGGTGTGACTCGTCGACCCTAACGCGTCGTACCCACGTCGCTCCCGACGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAA
## S1 2
## S2 0
## S3 4
## S4 3
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTTTAGTATTCTGAAGGGCATGCCTGTTCGAGCGTCATTATCACTAATCAAGCCCGGCTTGCTGTTGGACCTCTTGTCGTTAAAAGACAGGCCCCAAAGATAATGACGGTGTTATTAGAACCCTAGATGCAACGAGCTTTAACCAGCACGCATGATAGTGGCCTTTTAACCCGGTCTTTTTTAACCATTTTTTAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 4
## S6 38
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCGTTAGGCCGAGGGCACGCCTGCCTGGGTGTCACGCATCGTTGCCCCCTCCCAAACCCCCCTCTCCTCGAAAGGGGACGAGGGGACTTGGCCGTGGGCGGATATTGGCCTCCCGTCTGCCGAATGGCTCGCGGTTGGCCTAAATACGAGTCGTCGGCGACGGACGCCGTGACGTTCGGTGGTCAAAGAAACCTCGAGCTCCCGTCGCGCGTACGTCGTCGGTCCGATAAGGCTCACCGACCCTGAAGCGCTGTGGACAGCGCACGCACCGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAA
## S1 0
## S2 29
## S3 0
## S4 51
## S5 0
## S6 4
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTCGGTATTCCGTTGGGCATGCCTGTTCGAGCGTCATTTAATCATTCAAGCTCTGCTTGGTGTTGGGTGTTTGTTCCGCCTCAGCGCGTGGACTCGCCTTAAATTCATTGGCAGCCGGTATGTTGGCTTCGTGCGCAGCACATTGCGTCGCGATTCTGGCAGGCCTCCTCCCATTAAGCTTCTTTTAAGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTGTTGGGCTCGCCCTTTGGGGCCTGCCTCAAAATCAGTGGCGGCTCCGTCCGGTCTCAAGCGCAGTAATACTCGTCGCTTGTTGGATTCGGTCGGCGGCTTGCCAGCAACCCCCATTTTTACAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCACACCCGTTTGAGTGTCGTGAATACTCTCAACCTTCTTGGTTTCTTTGACCACGAAGGCTTGGACTTTGGAGGTTTTTCTTGCTGGCCTCTTTAGAAGCCAGCTCCTCCTAAATGAATGGGTGGGGTCCGCTTTGCTGATCCTCGACGTGATAAGCATCTCTTCTACGTCTCAGTGTCAGCTCGGAACCCCGCTTTCCAACCGTCTTTGGACAAAGACAATGTTCGAGTTGCGACTCGACCTTACAAACCTTGACCTCAAATCGGGTGAGACTACCCGCTGAACTTAA
## S1 172
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTGAATTCTCAAACCTGAATCTCCCCTGGTTCAGGCTTTGGATTTGGAGAGTGCCGGCCTTCAAACGAGTCCGGCTCCTCTGAAATGCATTAGCAAGACCCTCTTCCGACGGCTTTGTGGCATTGATAAGACTGTCCATGCCTATTTTTGTCCGGCGGAAAAGTGGTTGCTTTGAAATTGTCTGAAGCGAAACGGACACTTTACCATTGACCATTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 37
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATGAAATCTTCAACCTATAAGCTTTTGTGGTTTGTAGGCTTGGACTTGGAGGCTTGTCGGCCTTGATTGGTCGGCTCCTCTTAAATGCATTAGCTTGATTCCTTGCGGATCGGCTCTCGGTGTGATAATATCTACGCCGCGACCGTGAAGCGTTTGGCGAGCTTCTAACCGTCTCATTTGAGACACAACTTTATGACCTCTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 1
## S2 0
## S3 0
## S4 0
## S5 7
## S6 0
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCGACGCCATTCGGCCGAGGGCACGTCTGCCTGGGTGTCACGCATCGTTGCCCCCAAACGCCTCCCGCCTCGCAAGGGGCGCGGGATCTCGTCTGGCGGCGGAAGTTGGCCTCCCGTGGGCCTGTGCTCGCGGTTAGCCTAAAAAGGAGTCCTCGGCGACGAGCGCCACGACAATCGGTGGTTGATGAGACCTCGGTCCCCGTCGTGCGTGTCTGGTCGCCACACGGTGTGACTCGTCGACCCTAACGCGTCGTACCCACGTCGCTCCCGACGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAA
## S1 4
## S2 0
## S3 0
## S4 0
## S5 1
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCACAGCTTGGTCTTGGGCCGCGCCGCTAACCCGGCGGGTCTTAAAATCAGTGGCGGTGCCGTCGGGCCCTGAGCGTAGTACATCTCCTCGCTATAGGGTCCCGGCGGATGCTGGCCAGCAACCCCCAATCTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 8
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGTGTCATTAAATTATCAACCTTGCTCGCTTTTACCGGCTTGAGTTAGGCTTGGATGTGAGGGCTTTGCTGGCTTCCTTCAGTGGATGGTCTGCTCCCTTTAAATGCATTAGTGGGATCTCTTGTGGACCGTCACTTGGTGTGATAATTATCTATGCCACTTTGACCTTGAAGCAAAACTTATGGGAACCCGCTCATAACCGTCTTCGGACAACTTTTGACATTTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATTGAATCTTTGAACGCATCTTGCGCCTCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTAGAACTATCAAAAAAATAAATGATTTCGATCGTTAATTTTTTTGGAATTGGAGGTGGTGCTGGCCTTTTTCCATTAACGGCCCAAGCTCCTCCGAAATGCATTAGCGAATGCAGTGCACTTTTTCTCCTTGCTTTTTCTGGGCATTGATAGTTTACTCTCATGCCCTAAGCTGGTAGGGAGGAAGTCACAGAATGCTTCCCGCTCCTGAATGTAATACAAAACTTGACGATCAAACCCCTCAAATCAGGCAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 3
## S4 0
## S5 0
## S6 0
## ATGTGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCCTGGTCATTCCGGGGAGCATGCCTGTTTGAGTGTCATAATACTCTCAACCCCTGGGGTCTTTTAAACGGACCACCTCGGGCTTGGATCATGGACGCTGCCGGCCCCCCTCGGGGGTGTGGCTCGTCTGAAATACATGAGCTGACCTGTCTGCGAACAGCACGGTTTGACTCGGCGTAATAAATCCATTTTTCGTCGGGGACACCCTTCGCGGGGTGGCCGGGCTCGCGGTCTTCGGGTTGCTTACAATCGAATAGTCCCATCTATTTTAGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGTATTCTGGGGGGTATGCCTGTTCGAGCGTCATTGCAACCCTCAAGCACTGCTTGGTGTTGGATGTCATCACTGTGGTGCATCTCAAAAATATTGGCAGTGGCATTCAGCTTCTAGTGTAGTAAATTTCTTCACTATGGAGTCTGGGTGTCTGACTGCCAGAACCCTCAATTTATCAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 3
## S3 0
## S4 0
## S5 1
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCTGAGCGTCATTTCAACCCTCAAGCAACTTCTGCTTGGTGTTGGGGATCGGCCGTCAAACGCCGCCCCCGAAATCTATTGGCGATCTCGCTGTAGCTTCCTATGCGTAGTAGTAACATCTCGCATCTGGAGAGCGGCGCGGTCATGCCGTAAAACACCCAACTTTTCAGGTTGACCTCAGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 12
## S6 12
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCGACGCCATTCGGCCGAGGGCACGTCTGCCTGGGTGTCACGCATCGTTGCCCCCAAACGCCTCCCGCCTCGCAAGGGGCGCGGGATCTCGTTTGGCGGCGGAAGTTGGCCTCCCGTGGGCCTGTGCTCGCGGTTAGCCTAAAAAGGAGTCCTCGGCGACGAGCGCCACGACAATCGGTGGTTGATGAGACCTCTGTCCCCGTCGTGCGTGTCTGGTCGCCACACGGTGTGACTCGTCGACCCTAACGCGTCGTACCCACGTCGCTCCCGACGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAA
## S1 1
## S2 0
## S3 0
## S4 0
## S5 1
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCCAGCCTCGCTGGGTCTTGGGCTCTGCCAGCAATGGCTTGCCTTAAAATCAGTGGCGGTGCCGACGAGCTCTACGCGTAGTAATAAACCCCTCGCGTCTGAGTCTCGGCGGTTACTTGCCAGCAACCCCCAATTTTTCTGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATAACCAATCCAGCCTGCTGGGTCTTGGGCTTCGCCGGTCGGCGGGCCTCAAAGTCAGTGGCGGCACGGCCGGGCTCTAAGCGTAGTAATACTCCTCGCTATAGGGTCCCGGGCGCGGCTTGCCAGCAACCCCCCCACTTCTTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCGACGCCATTCGGCCGAGGGCACGTCTGCCTGGGTGTCACGCATCGTTGCCCCCAAACGCCCCCGCCTCGCAAGCGGCGCGGGATCTCGTTTGGTGGCGGAAGTTGGCCTCCCGTGGGCCTGTGCTCGCGGTTAGCCTAAAAAGGAGTCCTCGGCGACGAGCGCCACGACAATCGGTGGTTGATGAGACCTCGGTCCCCGTCGTGCGTGTCTGGTCGCCACACGGTGTGACTCGTCGACCCTAACGCGTCGTACCCACGTCGTTCCCAACGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTCTGCTTGGTATTGAGTCTATGTCAGTAATGGCAGGCTCTAAAATCAGTGGCGGCGCCGCTGGGTCCTGAACGTAGTAATATCTCTCGTTACAGGTTCTCGGTGTGCTTCTGCCAAAACCCCAAATTTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCGACGCCATTCGGCCGAGGGCACGTCTGCCTGGGTGTCACGCATCGTTGCCCCCAAACGCCCCCGCCTCGCAAGGGGCGCGGGATCTCGTTTGGTGGCGGAAGTTGGCCTCCCGTGGGCCTGTGCTCGCGGTTAGCCTAAAAAGAAGTCCTCGGCGACGAGCGCCACGACAATCGGTGGTTGATGAGACCTCGGTCCCCGTCGTGCGTGTCTGGTCGCCACACGGTGTGACTCGTCGACCCTAACGCGTCGTACCCACGTCGCTCCCAACGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAA
## S1 0
## S2 0
## S3 2
## S4 2
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATAACCACTCAAGCTTCGGCTTGGTCTTGGGGTTTCGAGGTTTCTCGGCCCTTAAAATCAGTGGCGGTGCCATCTGGCTCTAAGCGTAGTAATTTTTCTCGCTACAGGGTCCAGGTGGTTACCTGCCAATAAACCCCCCCTTTTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 3
## S5 4
## S6 3
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCCGTTCGAGCGTCATTATAACCCTTCAAGCTCAGCTTGGTGTTGGGGCCTGCTGTTACCGGCAGCTCTTAAAATCAGTGGCGGTGCCATCTGGCTCTAAGCGTAGTAACACTTCTCGCTACAGGGTCCCGGTGGATGCTTGCTATCAACCCCTTAACTTCTATGGTTGACCTCGGATCGGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 5
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATAACCACTCAAGCCCTGGCTTGGTCTTGGGGTTCGCGGTCCGCGGCCCTTAAACTCAGTGGCGGTGCCATCTGGCTCTAAGCGCAGTAATTCTCTCGCTATAGTGTCTAGGTGGTTGCTTGCCATAATCCCCCATTTTTTTTACGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 6
## S2 0
## S3 0
## S4 8
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTGTGGTATTCCGCAGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTCTGCTTGGTGTTGGGCCCCGCCCGCTCGCGGCCGGCCCTAAAGACAGTGGCGGCGGCGTCTGGCTCCAAGCGTAGTACAGCTCTCGCTCTGGGGCTAGGCGGTAGCCTGCCAGAACCCCCCTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 7
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCGACGCCATTCGGCCGAGGGCACGTCTGCCTGGGTGTCACGCATCGTTGCCCCCAAACGCCCCCGCCTCGCAAGGGGCGCGGGATCTCGTTTGGCGGCGGAAGTTGGCCTCCCGTGGGCCTGTGCTCGCGGTTAGCCTAAAAAGGAGTCCTCGGCGACGAGCGCCACGACAATCGGTGGTTGATGAGACCTCGGCCCCCGTCGTGCGTGTCTGGTCGCCACACGGTGTGACTCGTCGACCCTAACGCGTCGTACCCACGTCGCTCCCGACGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAA
## S1 6
## S2 0
## S3 0
## S4 4
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATTGAATCTTTGAACGCATCTTGCGCCTCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTAGAACTATCAAAAAAATAGATGATTTCAATCGTTAATTTTTTTGGAATTGGAGGTGGTGCTGGTCTTTTTCCATTAATGGCCCAAGCTCCTCCGAAATGCATTAGCGAATGCAGTGCACTTTTTCTCCTTGCTTTTTCTGGGCATTGATAGTTTACTCTCATGCCCTAAGCTGGTAGGGAGGAAGTCACAGAATGCTTCCCGCTCCTGAATGTAAAACAAAACTTGACGATCAAACCCCTCAAATCAGGCAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGAATTCCGAGGGGCACACCCGTTTGAGTGTCGTGAACATCCTTCAACCTTTCTTGGTTTCTTGACCGGGGAAGGCTTGGACTTTGGGGGTTTTTCATTGCTGGCCTCCCTTGAAGCCAGCTCCTCCTAAATGGATTAGTGGGGTCTGCTTTGCCGATCCTTGACGTGATAAGATGTTTCTACGTCTTGGGTTTCGCACTGTCGCTTCAGACCCCGCTTCGAACAGTCTTGCATCTAAGACAGAGTTTTATAAACCTTGACCTCAAATCGGGTGAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAACCATCAAGCTTGGCTTGTCGTTGGACTCGTGGTCGTCATGACCGGTCCGAAAGATAATGGACGGCGCTGATGAGCCCTCGATGCAACGAGCTTTTAATAGCACGCATTGAACGGTTGGTCGTACCGGCCTCGCACTTTATTTTCTCAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 1
## S6 65
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCGACGCCATTCGGCCGAGGGCACGTCTGCCTGGGTGTCACGCATCGTTGCCCCCAAACGCCCCCGCCTCGCAAGGGGCGCGGGATCTCGTTTGGTGGCGGAAGTTGGCCTCCCGTGGGCCTGTGCTCGCGGTTAGCCTAAAAAGGAGTCCTCGGCGACGAGCGCCACGACAATCGGTGGTTGATGAGACCTCGGTCCCCGTCGTGCGTGTCTGGTCGCCACACGGTGTGACTCGTCGACCCTAACGCGTCGTACCCACGTCGCTCCCAACGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAA
## S1 4
## S2 0
## S3 1
## S4 4
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACCTTGCGCCTCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTGAATCCTCAAAAGAGAAAACTTAATGGTTTCTCTCTTTTGGAATTGGGGTGTGCTGGCCTTTTCTTGGAAAGCTCAGCTCCCCTGAAATACATTAGCGATGCGACATCCTCATGCTCCACGCTTCTCTGGCATTGATAGTCTTTTCATGCTTAGAGCGTGTTCTGCAGCTGTGGAAAGTTTTGCTCGCTCACAACTGATGGGTTTTTACCCCATCCTCTGACCAATTTAACCTCAAATCAGGTAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTTAGCTTGGTATTGAGTCTATGTCAGCAATGGCAGGCTCTAAAATCAGTGGCGGCGCCATTGGGTCCTGAACGTAGTAATTTCTCTCGTTACAGGTTCTCAGTGTGCTTCTGCCAAAACCCAAATTTTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 4
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATATGGCACCCTCTCTTGAAATAGGAGGGTATGCCTGTTTGAGTGTCATTAAACTCCTCAATACCATTTACTGGTGTTTGGATGTTGAAGGTCTGCTGGTGAATCCCATCATCAGCTCCTTTTAAATACATGAGCAGGACTACATGCAACCAGCTCCAATTTGATAAGCTTGTTATCTTTGTTGTTGCTGGGAGTGAATAATGTAATTGTCTGCTACTAGTTGTCCCTGACCATTGGGACTTGCCTAATAGGCAACCATTTGAACCCTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 20
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCTTCTGGTATTCTGGGAGGTATGCCTGTTCGAGCGTCATTGCAACCATCAAGCCTAGGCTTGGTATTGGATGCCACCGCTTGGTGCATTTCAAAATTAGTGGCGGTGCCATTCAGCTTCAAGCGTAGTAAATTTCTCGCTCCTGGAGTTTGTATGTTGTCTGCTAGAACCCCCTAATTTATCAAGGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 9
## S2 0
## S3 0
## S4 0
## S5 16
## S6 0
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCGTTAGGCCGAGGGCACGCCTGCCTGGGTGTCACGCATCGTTGCCCCACCCCAAACCACCCCTCCCCTCTCGAAAGAGAGAGACGAGGGGACTTGGCCGTGGGCGGATATTGGCCTCCCGTGTGCCGAACGGCCCGCGGTTGGCCTAAATACGAGTTGTCGTCGGCGATGGACGTCGTGGCGTTCGGTGGTCAAATAAACCTCGAGCTCCCGTCACGCGTACGTCGTCGGCCTAATAAGGCTCACCGACCCTGAAGCGTTGTGAACAGCGCACGCATCGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAA
## S1 0
## S2 8
## S3 0
## S4 0
## S5 11
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTGGGCGTCACCACTCGTGGTGTGCCTTAAAATCAGTGGCGGTGCCATTCGGCTTCAAGCGTAGTAATTTTTCTCGCTTCTGGAGCTTGGGTGTGTGCTTGCCAGCAACCCCCAATTTTTTTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 12
## S4 5
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACTCCTTGGTATTCCGAGGAGTATGCCTGTTTCAGTATCATGAGAACTCTCACATCTAACCTTGGGTTAAAATGTGGAACTGGGATGCGCCGGTTATCATAACGGCCCTCCTAAAATTCAGTTCTTGGCTGCTTCCTACTACAGCAGTTTGGCCTAATAGTTTTGAGTCTCCGATTCAAATCTTTGGCTAACATTTGCTTCAGTGACGTCAGCTTTGATACAGAAAACTCATTAAACCTTTGATCTGAAATCAGGTAGGGCTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGTGTCATTAAATTCTCAACCCAAAAAGCTTTTGCTAGCTCTTTTGTGGCTTGGATGTGGGGGTTTTTTGCTGGCTTCCTTCAGTGGATGGTCTGCTCCCCTTAAATGCATTAGTGGGATCTCTTGTGGACCGTCACTTGGTGTGATAATTATCTATGCCAGTTGACTTTGAAGCAAAACTTATGGGAACCCGCTCATAACCGTCTTCGGACAACTTTTGACATTTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGATCGGCGTTGGGGATCGGGACCCCTCACACGGGTGCCGGCCCCTAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACAACTCGCACCGGGAGCGCGGCGCGTCCACGTCCGTAAAACACCCAACTTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 1
## S2 0
## S3 13
## S4 1
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTCAGCTTGGCCTTGGGCCCTGCCGGCAACGGCTGGCCTTAAAATCAGTGGCGGTGCCATCGAGCTCTACGCGTAGTAATTATATCTCGCTTCTGAGTCTCGGTGGTTGCTTGCCAGTAACCCCCAATTTTTTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATCCCGAGGGGCATGCCTGTTCGAGCGTCATTTCACCACTCAAGCCTGGCTTGGTGTTGGGCGACGTCCCCTTTGGGGACGCGCCTCGAAACGCTCGGCGGCGTGGCACCGGCTTTAAGCGTAGCAGAATCTTTCGCTTTGAAAGTCGGGGCCCCGTCTGCCGGAAGACCTACTCGCAAGGTTGACCTCGGATCAGGCAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 22
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCGGCATCCCGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAGGCTCCGCCTGGCATTGGGGTCCTGCGCCTGCAGCCCCCGAAAGACAGTGGCGGGTGCGCCCTACGACCGAACGCAGTAGTGTATCTCGTTCTGGTTCGGGGCGCCCTCCGGCCGTAAAATCCCCACTCTCTCATGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 3
## S3 5
## S4 3
## S5 3
## S6 0
## ATGCGATAAGTAATGAGAATTGCAGAATTCAGTGGATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTCTGCTTGGTCTTGGGCCTGGCCATGATGGCCGGTCTTAAAATCAGTGGCGGTGCCGTCGCAGGCTCCAAGCGTAGTACATCTCTCGCTCTGGAAACTTGGCGGTGCCTGCCAGACAACCCCTAATTGTCTCTTTCGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 58
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCACGCCTAGCGTGGTATTGGGCTCGCCCTGTAGGGCTTGCCTCAAAGTCAGTGGCGGCACCGTTTGACCCCTAAGCGTAGTAATACTCGTCGCTTCTGGTGTTGTACGGAGGCCTGCCAGCAACCCCCAATTTTTAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCGTGGTATTCCGCGGGGCATGCCTGTTCGAGCGTCATTATGACCAATCCCGCTCGCGGGGTCTTGGGCACCGCCTCTGGGCGGGCCTCAAAATCAGTGGCGGTACGGCCGGGCTCTGAGCGTAGTAAATCTTCTCGCTACAGGGTCCCGGGCGGCACTGGCCAGCAACCCCCAATCTTTTACAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 1
## S3 0
## S4 0
## S5 0
## S6 0
## GCGCGATAGGTATTGTGAATTGCAGAATTGTGAATCATCGAATTTTTGAACGCACATTGCACCCATTGGTATTCCGATGGGTATACTTGTTTGAGCGTCATTTCATTCTCCTTTTGGGGTTTTAGCTTGATTAAATCTCGCAGAATTATAATGGTGTGGCTACCAGACTACAACGTGATAGATATTTCGTTGGATGTGACTGGGATTGCTCACCTTAAAACTAAAGTATAGACCTCAAATCAAGCAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCAGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCACGCCTAGCGTGGTCTTGGGCTCGCCCTGTAGGGCCTGCCTCAAAGTTAGTGGCGGCGTCGTCCGATCCCTAAGCGTAGTAAAATACACCTCGCTTTTGGGCGCTGGATGGAGGCTTGCTATGAACCCCCACTTTTACAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTTACACCACTCAAGCCTAGCTTGGTATTGGAGCTCGCACACCAGCGGCTCTTAAATTTAGTGGCGGCGCCGGTTGGCTCTTCGCGTAGTAATTCTCTCGCGATAGGGTCTGTCGGTAGCTTGCCAGCAACCCCAATTTTTTTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 57
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCTAGTATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCATCAAGCCCTAGGCTTGTGTTGGGGCCCTGCGGCCGTCCGCAGCCCCCTAAAAACAGTGGCGGGCTCGCTATATACCGAGTGCAGTAGTTTACTCTTCGCTCAGGACATGTAGTGGGTTCTTGCCGTAAAACCCCCCATTTTTAAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 76
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCACAGCTTGGTCTTGGGCTTCGCCGCTAACCCGGCGGGTCTTAAAATCAGTGGCGGTGCCGTCGGGCCCTGAGCGCAGTAATCTCTCTCGCTATAGGGTCCCGGTGGATGCTAGCCAGTAACCCCCAATTTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 3
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATAACCACTCAAGCTTCGGCTTGGTCTTGGGGTTTCGAGGTTTCTCGGCCCTTAAAATCAGTGGCGGTGCCATCTGGCTCTAAGCGTAGTAATTTTTCTCGCTATAGGGTCCAGGTGGTTACCTGCCAATAAACCCCCCCTTTTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 1
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGCATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCGCGCCCATCCGGGCTGCGGTGTTGGGGCGGGCGGCTGGACGCAAGTCCGGCTGCCGCCCCCAAATACAGTGGCGGCCTCGCTGCACTTGCTCTGCGTAGTAGCACACCTCGCATTGCAAAGTAGCGCGCCCACGCCGTAAAACCCCCGACCCCCATGAGTTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGCGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCACCTGGCCGAGGGCACGTCTGCCTGGGTGTCACGCATCGTTGCCCCCAACCCCATCTCCTTGTAAAGGGACGAGGGGGCCTGTGGGGCAGAAATTGGCCTCCCGTGAGCTCATGCATGCGGTTGGCCTAAAAGCGAGTCCTCGGCGACGCGCGCCACGACAATCGGTGGTTGTCAAACCCTCGTGTCCCGTCGTGCGTGACGCGTCGCTCATCGTGTGCTCCTTGACCCTGCTGTGTCGCGCTAGCGACGCTTCCAACGCGACCCCAGGTCAGGCGGGACTACCCGCTGAATTTAA
## S1 0
## S2 0
## S3 0
## S4 19
## S5 4
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCACACCTGTTCGAGCGCCATTTCAACCCTCAAGCCCTGCTTGGTGATGGACCCCGCCTGCGGGCGGGTCCGAAACTCGTGGGCGTCGTCGTTCGGCTCCAAGCGTAGCAAAACCCTCGCTTGGGCGCCCGGACGGCGGCCGCCCGGAACGAATTTTTACAAGGTTGGCCTCGGATCAGGTGGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGCATTCCGAGGGGCATGCCTGTTCGAGCGTCATTGCAACCCTCAAGCTCTGCTTGGTATTGGGCATCACCAGCCTTGGTGAGCCTTAAATGCAGTGGCGGTGCCGTCTGGCTCTAAGCGTAGTAGTAATTTTCTTGCTATAGGGACCAGGTGGATGCCTGCCATCAACCCCTCTGTTTTATCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCGACGCCATTCGGCCGAGGGCACGTCTGCCTGGGTGTCACGCATCGTTGCCCCCAAACGCCCCCGCCTCGCAAGGGGCGAGGGATCTCGTTTGGTGGCGGAAGTTGGCCTCCCGTGGGCCTGTGCTCGCGGTTAGCCTAAAAAGGAGTCCTCGGCGACGAGCGCCACGACAATCGGTGGTTGATGAGACCTCGGTCCCCGTCGTGCGTGTCTGGTCGCCACACGGTGTGACTCGTCGACCCTAACGCGTCGTACCCACGTCGCTCCCGACGCGACCCCAGGTCAGGCGAGACTACCCGCTGAGTTTAA
## S1 3
## S2 0
## S3 0
## S4 2
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATTGAATCTTTGAACGCATCTTGCGCCTCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTAGAACTATCAAAAAAATAGATGATTTCGATCGTTAATTTTTTTGGAATTGGAGGTGGTGCTGGTCTTTTTCCATTAATGGCCCAAGCTCCTCCGAAATGCATTAGCGAATGCAGTGCACTTTTTCTCCTTGCTTTTTCTGGGCATTGATAGTTTACTCTCATGCCCTAAGCTGGTAGGGAGGAAGTCACAGAATGCTTCCCGCTCCTGAATGTAATACAAAACTTGACGATCAAACCCCTCAAATCAGGCAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 59
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATGAAATTCTCAACCTTTTCAGCATTTTATGTTGATCTGGCTTGGATGTGGGAGTTTGCGGGCTTCTCAGAAGTCGGCTCTCCTTAAATACATTAGCGGGACCTTTGTTGCCCAGCTTTAGTGTGATAATTATCTACGCTATTGTTGAAAGCAGCTTTTAATGGGTACAGCTTCTAATCGTCTCATTTGAGACAGCTTCTGACAATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAATCATTGAGTCTTTGAACGCAAGTTGCGCCCGACGCCCTTCGGCCGAGGGCACGTCTGCCTGGGTGTCACGCATCGTTGCCCCCAAACGCCCCCGCCTCGCAAGGGGCGCGGGATCTCGTTTGGTGGCGGAAGTTGGCCTCCTGTGGGCCTGTGCTCGCGGTTAGCCTAAAAAGGAGTCCTCGGCGACGAGCGCCACGACAATCGGTGGTTGATGAGACCTCGGTCCCCGTCGTGCGTGTCTGGTCGCCACACGGTGTGACTCGTCGACCCTAACGCGTCGTACCCACGTCGCTCCCAACGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGTGTCATTAAATTCTCAACCCAAAAAGCTTTTACTAGCTCTTTTGTGGCTTGGATGTGGGGGTTTTTGCTGGCTTCCTTCAGTGGATGGTCTGCTCCCCTTAAATGCATTAGTGGGATCTCTTGTGGACCGTCACTTGGTGTGATAATTATCTATGCCAGTTGACTTTGAAGCAAAACTTATGGGAACCCGCTCATAACCGTCTTCGGACAACTTTTGACATTTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCTCCTGGTATTCCGGGAGGCATGCCTGTTCGAGCGTCATTATGACCACTCACGCTCCGCGTGGATTTGGGGCTCGCTGTACCGGCGGCCCTTAAAAGCAGTGGCGGTGCCGTCTGGCTCTACGCGTAGTAATTTCTCTCGCGATTGGGTCTTACGGTTGCTTGCCAACAACCCCCAACTTTTTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATATTGCACCCTCTGGCATTCCGGAGGGTATGCCTGTTCGAGCGTCATTACAGCCCTCAAGCACTCTGCTTGGAGTTGGGCGCCGCCGGCCTACGGCGCGCTTCAAAATCAGTGGCGGTGTCGGACGGCCCCGAGCGTAGTAAATCTCTCTCGCTTTGGGGCCTGGACGGAGCCTGCCAGAAGCGATTACACCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 24
## S3 0
## S4 0
## S5 0
## S6 32
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAACCATCAAGCCTGGCTTGTCGTTGGACCCCTTTGCCATTGAAATATATGACAGGTCCGAAAGATAATGACGGCGTCGTGTTTGACCCTAAATGCAACGAGCTTTTTATAGCACGCATTGAAGTGGTCGGCCGACCCAGTCTTTAACCATCATTTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 14
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATCCCGAGGGGCATGCCTGTTCGAGCGTCATTTCACCACTCAAGCCTGGCTTGGTGTTGGGCGACGTCCCCTTTGGGGACGCTCCTCGAAACGCTCGGCGGCGTGGCACCGGCTTTAAGCGTAGCAGAATCTTTCGCTTTGAAAGTCGGGGCCCCGTCCGCCGGAAGACCTACTCGCAAGGTTGACCTCGGATCAGGCAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATAACCAATCCAGCCCCGCTGGGTCTTGGGCTCCGCCTCTGGGCGGGCCTTAAAATCAGTGGCGGTGCCGTCTGGCTCTAAGCGTAGTAATTCTCTCGCTATAGGGTCCAGAGGGATACCAGCCAGCAACTCCCACTTCTCAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 1
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATGAAATCTTCAACCTATAAGCTTTTGTGGTTTGTAGGCTTGGACTTGGAGGCTTGTCGGCCTTGATTGGTCGGCTCCTCTTAAATGCATTAGCTTGATTCCTTGCGGATCGGCTCTCGGTGTGATAATATCTACGCCGCGACCGTGAAGCGTTTGGCGAGCTTCTAACCGTCTCACTTGAGAGACAACTTTATGACCTCTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 3
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTACACCACTCAAGCACCGCTTGGTATTGGGCATCGTCCGTCGAAAGGCGGGCGTGCCTCGAAGACCTCGGCGGGGTTTCTCCAACTTCGGGCGTAGTAGAGTTAAATCAAAACGTCTTATAAGCTTGGTGAGATCTCATTGCCGTTAAACCTTTTATTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 1
## S6 10
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTGTTGGGCCCCGCCGCCCCGGCGGGCCCTAAAATCAGTGGCGGTGCCGTCCGGCTCCGAGCGTAGTAATTCTTCTCGCTCTGGAGGTCCGGGTGTGTGCTCGCCAGCAACCCCCAATTTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 2
## S2 0
## S3 0
## S4 31
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATAACCACTCAAGCCCCGGCTTGGTCTTGGGGTTCGCGGTCCGCGGCCCTTAAACTCAGTGGCGGTGCCGTCTGGCTCTAAGCGCAGTAATTCTCTCGCTATAGTGTCTAGGTGGTTGCTTGCCATAATCCCCCAATTTTTTACGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 37
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTGAATTCTCAACTTGGCCCGAGTTTGCTCTCGGAGCTCAAGCTTGGATTTGGAGTGTGCTGGTCCCTTGCGGGGTCGGCTCCTCTGAAAAGCATTAGCGACGAAATCCTTTCAAGGGTTGGCCCCAAAATGTGCTGTGGCATTGATAACAATTGTCTATGTCTCACTGCGGGGGGGGAGGATGACCCAAGGGCGTTCTCGCTTACAATGGTCCTCGTCGTGGGGACAAAGGCTCACCTTGACAATTACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTTGAGTGTCATGTAGACTCAATCCCTCGGGTTTCCGAGGAGATTGGACTTGGGTGTTGCCGCTCTGCCGGCTCGCCTTAAAAGACTTAGCGGGATAGCACCGTAGTCGGCGTAATAAGTTTCGTCGGTGAAGGTTGTGATGACTGCTTACAATCGCCCTCGGGCAATTTTTGACTCTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAACCATCAAGCCTGGCTTGTCGTTGGACCTCTTTGCCACTGAAATATGTGACAGGTCCGAAAGATAATGACGGCGTCGTGTTTGACCCTAAATGCAACGAGCTTTTTATAGCACGCATTGAAGTGGTCGGCCGACCCAGTCTTTAACCATCATTTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 1
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAACCATCAAGCCTGGCTTGTCGTTGGACCCCCTTTTGCCATTGAAATATGTGGTAGGTCCGAAAGATAATGACGGTGTCGTGTTTGACCCTAGATGCAACGAGCTTTTAATAGCACGCATTGAAGTGGTCGGCCGACCCGGTCTTAAACCATTATTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCTTCTGGTATTCCGGGAGGTATGCCTGTTCGAGCGTCATTGCAACTAACAAGCCCAAGCTTGGTATTGGATGTCATCTAGCGGGTGCATCTTAAAATCAGTGGCGGTGCCATTCAGCTTCAAGCGTAGTAAATTTCTCGCTCCTGGAGTTTGGGTGTTGTGCCTGCCAGAACCCCTAAATTATCAAGGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 43
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCATAGCTTGGTATTGGGCTCCGCTACTCACCTAGCGGGCCTTAAAATCAGTGGCGGTGCCGTCGAGGCCCTGAGCGTAGTAAATATCCTCGCTATAGGGACCCGGCGGACGCTGGCCATCAACCCCCAACTTTCTAAGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 1
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCACACCCGTTTGAGTGTCATGAATACTCTCAACCTTCTTGGTTTCTTTGACCACGAAGGCTTGGACTTTGGAGGTTTTTCTTGCTGGCCTCTTTTGAAGCCAGCTCCTCCTAAATGAATGGGTGGGGTCCGCTTTGCTGATCCTCGACGTGATAAGCATTTCTTCTACGTCTTAGTGTCAGCTCGGAACCCGCTTTCCAACCGTCTTTGCACAAAGACAATGTTCGAGTTGTGACTCGACCTTACAAACCTTGACCTCAAATCGGGTGAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGCATTCCGAGGGGCACACCCGTTTGAGTGTCGTGAAATCCTCAAAACCTTTTTCTTTGACGAGAAAAGGAGTTTTGGACTTGGAGGTTCAATGCCCGCTTTCGGCATCGAAAGCGAGCTCCTCTCAAATGAATCAGTGGGGTCCGCTTTGCTGGTCCTTGACGTGATAAGATGTTTCTACGTTTTGGATCTGGCACTGTCCCTTGGATGCCCGCTTCTAACCGTCTCATAGACAACGATGGTGCCCCGGTCACCTCCGTCCACGTTGGCGGGGGAGGCTTGGCCCACGACAAAAAAACCTTGACCTCAAATCGGGTGAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATATGGCACCCTCTCTTGAAATAGGAGGGTATGCCTGTTTGAGTGTCATTAAACTCCTCAATACCATTTGAATGGTGTTTGGATGTTGAAGGTCTGCTGGTAATTCCATCATCAGCTCCTTTTAAATACATGAGCAGGACTACATGCAACCAGCTCCAATTTGATAAGCTTGTTATCTTTGTTGTTGCTGGGAATGGATAATGTAATTGTCTGCTACTAGTTGTCCTGACCATTGGGACTTGCCTAATTGGCAACCATTTGAACCCTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGCATTCCGAAGGGCATGCCTGTTCGAGCGTCATGATCAAACCTCAAGCCTTGCTTGGTATTGGGCTTTCGCCTTCCCTATCTTGTTTACAGGTAGTGGCGTGCCTGAAAGTCAATGGCAGTGTCCTCAATAGACTCAAGCGTAGCAGACTGACTCGCTTTTGAGAGCTTTTGTAGGTCCCGTCAACAAAATAATCAAGTTGGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 51
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAACCATCAAGCCTGGCTTGTCGTTGGACCTCTTTGCTGCTGAAATATGTGGCAGGTCCGAAAGATAATGACGGCGTCGTGTTTGACCCTAGATGCAACGAGCTTTTTTATAGCACGCATTGATGTGGTCGGGCGACCCAGTCTTTAACCATTATTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 5
## S5 53
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCACGCCTAGCGTGGTATTGGGCTCGCCCTGTAGGGCTTGCCTCAAAGTCAGTGGCGGCACCGTTTGACCCCTAAGCGTAGTAATACTCGTCGCTTCTGGTGTTGTGCGGAGGCCTGCCAGCAACCCCTATTTTTTAAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGCATTCCGAGGGGCACACCCGTTTGAGTGTCGTGAAATTATCAAAACCCTTTTCTTTGATCCTTTTTGGAGCGGAAAAGGACTTTTGGACTTGGAGGTTCAATGCTCGCTTTCACCTATGAAAGCGAGCTCCTCTCAAATGAATTAGTGGGGTCCGCTTTGCCGATCCTTGACGTGATAAGATGTTTCTACGTTTTGGGTTTGGCACTGTCCCTTGGATGTCCGCTCCTAACCGTCCGTCTCATGGACAATGATGGTGCTCTGGTCGCCGGCCACCTACGTTGGCGGGAGGCTGGACCCACAAAAAACCTTGACCTCAAATCGGGTGAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 31
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTCTTGGGCGTCACCGGTCCCCGGTGTGCCTTAAAATCAGTGGCGGCGCCATCTGGCTCTAAGCGTAGTACATACTCTCGCTACAGACGTCCGGTGGATGCTGGCCAGCAACCCCCAATTTATCAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTTTGACCAACTCCTATTCTTTTATAGGGTCTTGGGTTATGCCTTTCTAGGCAGGCCTTAAAACCAGTGGCGGTGCTATATAGCTCTACGCGTAGTAATACTTCTCGCTATAGATGTTATATGGTATCTTGCCAACAACCCCCAACTTCTTAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTCAGCTTGGTCTTGGGCCCTGCCGGCAACGGCTGGCCTTAAAATCAGTGGCGGTGCCATCGAGCTCTACGCGTAGTAATTATGTCCTCGCTTCTGAGTCTCGGTGGTTGCTTGCCAGTAACCCCCCAATTTTTTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATATTGCGCTCTCTGGTATTCCGGAGAGCATGCTTGTTTGAGTATCAGTAAACACCTCAAATCCCTCTACTTGTTGGAGGAATTTGGACTTGAGCAATCCCAACTTTTCGAAAGAGAAGGCGGGTTGCTTGAAATGCAGGTGCAGCTAGATTCTTTCTTCTGAGCTATAAGCATACTTATTTAATCCCGTCAAACGGATTATTACTTTTGCTGCAGCTAACATAAAGGTTGAAGAGTCGCTATGGCTGACTGATGCAGGATTTAACATGTCGCGCAAGCGATCTGTAAACTCGATCTCAAATCAAGCAAGACTACCCGCTGAACTTAA
## S1 1
## S2 0
## S3 14
## S4 0
## S5 0
## S6 0
## ATGTGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCCTAGTATTCTGGGGAGCATGTCTGTTTGAGTGTCATGAACTCTTCAACCCACTGGTTTCTTGTAGACTAGCTGGTGTTTGGATTTTGAACGTTGCTGGTCTTCGGACGTAGCTCGTTCGTAATACATTAGCATCTCTAATTCGAATTCGGATTGACTCAGTGTAATAGACTATTCGCTGAGGACACTTTTATTAGTGGCCGAACAAGATCATTGTAGAAGCTTCAAACCTCTATAGTCAATTTTAGATTAGACCTCAGATCAGGCAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTGCAACCCTCAAGCTATGCTTGGTATTGGGCTCCGTCTACTACTGACGGGCCTTAAAATCAGTGGCGGTGCCATTAGGCTCTAAGCGTAGTAATTTTCTCGCTATGGACGTCTGGTGGATACTTGCCAGTAACCCCAAATTTTTAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTGGGCTACACCCGACTGGGTGGGCCTTAAAATCAGTGGCGGTGCCATCTGGCTCTAAGCGTAGTAATTCTTCTCGCTCTGGAGATCTAGGTGTTTGCTTGCCAGCAACCCCCAATTTATCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 2
## S2 23
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTTTAGTATTCTGAAGGGCATGCCTGTTCGAGCGTCATTATCACTAATCAAGCCTGGCTTGCTGTTGGACCTCTTGTCGTTAAAACTGACAGGCCCTAAAGATAATGACGGCGTTACTTGGACCCTAGATGCAACGAGCTTTCATTAGCACGCGTTTAGTCGGACCTTTTAACCCGGTCTTTTTAACCAATTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 2
## S5 2
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGTATTCCGGGGGGTATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCCCAGCTTGGTATTGGGTGTCACCTGGCAATGGTGTACCTTAAAATCAGTGGCGGTGCTGTCTGGCTTCAAGCGTAGTAAAACAATCGCTTTGGAGGTTTGGATGTGTTCTTGCCATCAACTCCTAATTTATCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCACTTCAACCCTCAAGCTCTGCTTGGTATTGGGCCGCACCAGCAATGGTAGGCCTTAAAATCAGTGGCGGCGCCGCTGGGCCCTGAGCGTAGTAATACTCCTCGCTACTGGGTCCCGGTGGATGCTTGCCATCAACCCCCAATTTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATTGAATCTTTGAACGCATCTTGCGCCTCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTAGAACTATCAAAAAAATAGATGATTTCAATCGTTAATTTTTTTGGAATTGGAGGTGGTGCTGGTCTTTTTCCATCAATGGGCCCAAGCTCCTCCGAAATGCATTAGCGAATGCAGTGCACTTTTTCTCCTTGCTTTTTCTGGGCATTGATAGTTTACTCTCATGCCCTGAGCTGGTAGGGAGGAAGTCACAGAATGCTTCCCGCTCCTGAATGTAATACTAAACTTGACGATCAAACCCCTCAAATCAGGCAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTTAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCATTAGTATTCTAGTGGGCATGCCTGTTCGAGCGTCATTTCAACCCCTTAAGCCTTGGTTGCTTAGTGTTGGAAGCCTACCGCATTCTATGCTGTAGCTTCTTAAAGTTAGTGGCGGAGTCGGTTCATACTCTAGGCGTAGTAAAGATTTTATTCTCGCCTGTAGAGATGAGCCGGTCCCCTGCCGTAAAACACCCCCCTATTTTTTATAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 1
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCACACCCGTTTGAGTGTCGTGAAAACCTCAACCTCCTTTGGTTTTCCTTCTGGAGACCGAAGGAGGCTTGGACTTTGGAGGCCTTTGCTGGTGTCTCTCACCAGCTCCTCTTAAATGAATTAGCGGGGTCCTCTTTGCCGATCCTCGACACGTGATAAGATGCTTCCGTGTCTCGGTTTCTGGCTCTGTTGCTTTTGGGACCCGCTTCTAACCGTCTGGACCCTTGCGTCGAGACAATGTTCGAGCTTGTCTCCCTTCTCGGGAAACCCCCTCGACCCCACGAACCCTTGGCCTCAAATCGGGTGAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATAAGTAATGTGAATTGCAGATTTTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTGAATTCTCAACCATGTCTTGGTTGGATTTGGGAGTTGCTGGCCTTGTCAGCTCTCCTGAAAAGCATTAGCAAAAGTCTTTTGGCCTCTGACGTGATAATGATCGTCGTGGGCTGGGAGCAGCATGGCTTCAATCTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCTTCTGGTATTCTGGGAGGTATGCCTGTTCGAGCGTCATTGCAACCATCAAGCCTAGGCTTGGTATTGGATGCCACCGCTTGGTGCATCTCAAAATTAGTGGCGGTGCCATTCAGCTTCAAGCGTAGTAAATTTCTCGCTCCTGGAGTTTGTGTTGTGCCTGCTAGAACCCCTAATTTATCAAGGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATAACCACTCAAGCCTAGCTTGGTATTGGGGTTCGCGTGCTCGCGGCCCTTAAAATCAGTGGCGGTGCCGTCTGGCTCTAAGCGTAGTAATTCTCTCGCTATAGAGTCCCGGCGGTTACCTGCCAGAACCCCCCACTTTTTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 5
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTTAAACCAATCCAGCGTGCTGGGTCTTGGGCTTTCGCCTCTGGGCGGGCCTCAAAGTCAGTGGCGGTGCTGCCAGGCTCTAAGCGTAGTAATTCTTCTCGCTACAGGGTCCTGGTGGAGGCTTGCCAATAACCCCAAATATTTCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCAAATCTTTGAACGCACATTGCGCTCCCTGGTATTCCGGGGAGCATGCCTGTCTGAGCGTCATTACACACCTTGCGCCTTGCGCAGTGTTGGGCGCAGCCTTCAGGGGCTCGCCTCAAATCCAGTGGCAGCACCTTCAGAGCTCCTACGCGCAGTGATCACCGCCGCCGGTGGAGTCCTGTGGGCGCTAGCTAGTCAACCCCCATTTGCTAGGTTGACCTCAGATCAGGCAGAGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCACACCTGTTCGAGCGCCATTTCAACCCTCAAGCCCCGCTTGGTGATGGGCCCCGTCCGAACGGGCGGGCCCGAAACTCGCAGGCGCCACCGTCCGGCCTCAAGCGTAGCAAAACCCTCGCTTGGGCGCTCGGTCGGCGGCTGCCCCGAACGAATTTTCACAAGGTTGGCCTCGGATCAGGTGGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 2
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTGGGCGTCACCACCTGGTGCGCCTCAAAAACAGTGGCGGTGCCGTCTGGCTTCAAGCGTAGTAATTTTTCTCGCTCCGGAGTCCAGGCGAGTGCTCGCCAGAACCCCCAATTTATCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 5
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCCGTTCGAGCGTCATTATAACCCCTCAAGCCCAGCTTGGTGTTGGGGCCTGCTGTTACCGGCAGCCCTTAAAATCAGTGGCGGTGCCATCTGGCTCTAAGCGTAGTAATACTTCTCGCTACAGGGTCCTGGTGGATGCTTGCCATCAACCCCTAAATTTCTATGGTTGACCTCGGATCGGGTAGGGATACCCGCTGAACTTAA
## S1 2
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAACCATCAAGCCTGGCTTGTCGTTGGACCCCTTTGCGACTGAAATATGTGGCAGGTCCGAAAGATAATGACGGCGTCGTGTTTGACCCTAAATGCAACGAGCTTTTCATAGCACGCATTGAAGTGGTCGGCCGACCCGGTCTTTAACCATCATTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATTGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTGAACCCCCTCAACTTGACCTTTTAGGTTGAGCTTGGATTTGGAGAGTGCCGGCGAGTTTCGGCTCCTCTGAAAAGCATTAGCGAGCGACCATGGCGTCTGCTTTGGCATTGATAAACCTGTCTATGCCCTGGGCGCGCTTGACGGCGTTCGCTTACAGTGGTCCCACAGAGGACGAACCGTAACCCAACAATCACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATGACCACTCAAGCCTAGCTTGGTATTGGGGTTCGCGGCGTCGCGGCCCCTAAAATCAGTGGCGGTGCCGTCTGGCTCTAAGCGTAGTAAATTTTCTCGCTACAGGGTCCGGAGGGTGGCTTGCCAACAACCCCCAACTCTTACGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 10
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCTCCCTGGTATTCCGGGGAGCATGCCTGTTCGAGCGTCATTACAACCCTCACGCTCCGCGTGGTGTTGGGCTCGCCCTCCAGGGCTTGCCTCAAACTCAGTGGCGGCACCGTCTGACCCCTAAGCGTAGTAATACTCGTCGCTTCTGGTAGTTGGATGGAGGCCTGCCAGGAACCCCCATTTTTACAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 1
## ATGTGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCCTGGTCATTCCGGGGAGCATGCCTGTTTGAGTGTCATAATACTCTCAACCCCTGGGGTCTTTAAGCGGACCACCTCGGGCTTGGATCATGGACGCTGCCGGCCCTCTCGGGGGTGTGGCTCGTCTGAAATACATGAGCTGACCTGTCTGCGAACAGCACGGTTTGACTCGGCGTAATAAATCCATTTTTCGTCGGGGACACCCTTTGCGGGGTGGCCGGGCTCGCGGTCTTCGGGTTGCTTACAATCGAATAGTCCCATCTATTTTAGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 8
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGCCTTGGGCGTCACCGGTAACGGTGGGCCCCAAACCCAGTGGCGGTGCCGTCTGGCTCTAAGCGTAGTAATTCTTCTCGCTATAGATGCCCGGTGGATGCTTGCCAGTAACCCCCAATTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTGAATTCTCAAACCTGAATCTCCCCTGGTTCAGGCTTTGGATTTGGAGAGTGCCGGCCTTCAAACGAGTCCGGCTCCTCTGAAATGCATTAGCAAGACCCTCTTCCGACGGCTTTGTGGCATTGATAAGACTGTCCATGCCTATTTTTGCCCGGCGGAAAAGTGGTTGCTTTGAAATTGTCTGAAGCGAAACGGACACTTTACCATTGACCATTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCTTCTGGTATTCTGGGAGGTATGCCTGTTCGAGCGTCATTGCAACCATCAAGCCTAGGCTTGGTATTGGATGCCACCGCTTGGTGCATCTCAAAATTAGCGGCAGTGCCATTCAGCTTCAAGCGTAGTAAATTTCTCGCTCCTGGAGTTTGTGTTGTACCTGCTAGAACCCCCTAATTTATCAAGGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 7
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCACAGCTTGGTCTTGGGCTTCGCCGCTAACCCGGCGGGTCTTAAAATCAGTGGCGGTGCCGTCGGGCCCTGAGCGCAGTAATCTCTCTCGCTATAGGGTCCCGGCGGATGCTGGCCAGTAACCCCCAATTTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATGACCACTCAAGCCTAGCTTGGTATTGGGGTTCGCGGTTCCGCGGCCTCTAAAATCAGTGGCGGTGCCGTCTGGCTTCAAGCGTAGTAATTTTCTCGCTACGGAGTCCGGGTGGTTGCTTGCCAAAACTCCCCAACTTCTCAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 3
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTTAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTGGGCCGCGCCACCTGGCCGGCCCCAAAGACAGTGGTGGCGCCCCCCGGCCCTCAGCGTAGTACATTTCTCGCTGTAGGACCCGTGGCGGTGTCGACACCCTAAACGCACAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTTTGCTTGGTATTGGGCATCACTATCCTAGGTGTGCCTCAAAGTCATTGGCGGTGCCACCTGGCTTCTAGCGTAGTAATACTTTTCGCTATGGAGTTTAGGTGAGTGCTTGCCACCAACCCCTAATTTCAAGATTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAACCATCAAGCCTGGCTTGTTGTTGGACCCCTTTGCAACTGAAACATGTAGCAGGTCCGAAAGATAACGACGGTGTCATGTTTGACCCTAGATGCAACGAGCTTTTCATAGCACGCATTGATGTGGTTGACTGACCCGGTCTTAACTATATTTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 3
## S3 0
## S4 0
## S5 0
## S6 1
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTGTGGTATTCCGCAGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCCTCGCTTGGTGTTGGGCCCTGCCCGCCGCGGCCGGCCCTAAAGATAGTGGCGGCGCCGCCTGGCCCTCAGCGTAGTACAGCTCTCGCTCCAGGGTCCGGCGGCAGCCTGCCAGAACCCCCCCCAACTCTGTGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 7
## S5 3
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATGACCAATCAAGCCTGGCTTGGTGTTGGGGTTCGCGGTCTCGCGGCCCTTAAAATCAGTGGCGGTGCCGCCTGGCTCTAAGCGTAGTAATTTTCTCGCTATTGGGTCCGGCGGTTGCTTGCCAGAATCTCCATCTCACGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 3
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCTTCTGGTATTCTGGGAGGTATGCCTGTTCGAGCGTCATTACAACTATCAAGCTCAAGCTTGGTATTGGATGCCACGTTTTAGTGCATCTCAAAATCAGTAGTAGTGCTATTCAGCTTCAAGCGTAATAATTTTCTCGCTTCTGGAGCATGTTTTGTACTTGCTTAGAACCCCTAATGTATTAAGGCTTGACCTCGGATCAGGTAAGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCTCGCCAGTATTCTGGCGAGCATGCCTGTTCGAGCGTCATTTCAACCATCAAGCTCTGCTTGCGTTGGGGATCCGCGGCTGCCCGCGGTCCCTCAAAAACAGTGGCGGGCTCGCTAGTCACACCGAGCGTAGTAACTCTACATCGTTCTGGTCGTGCGGCGGGTTCTTGCCGTAAAACCCCCCAATTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCACACCCGTTTGAGTGTCGTGAATACTCTCAACCTTCTTGGTTTCTTTGACCACGAAGGCTTGGACTTTGGAGGTTTTTCTTGCTGGCCTCTTTTGAAGCCAGCTCCTCCTAAATGAATGGGTGGGGTCCGCTTTGCTGATCCTCGACGTGATAAGCATTTCTTCTACGTCTTAGTGTCAGCTCGGAACCCGCTTTCCAACCGTCTTTGCACAAAGACAATGTTCGAGTTGTGACTCGACCTTACAAACCTTGACCTCAAATCGGGTGAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATAACCACTCAAGCCTCGGCTTGGTCTTGGGGTTCGCGGTCTCGCGGCCCTTAAAATCAGTGGCGGTGCCGTCTGGCTCTAAGCGTAGTAATTCTCTCGCTATAGGGTCCCGGCGGTTACCTGCCAGAACCCCCCATTTTTTACGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 1
## S5 2
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTTTGTGAATCATCGAATCTTTGAACGCACATTGCACCCCCTGGTATTCCGGGGGGTACACCTGTTCGAGCGCCGTTTAAGTCTTGAGGCTCCGCCTTGTGTTGACTCTCCCCTTTTTGGGGTAGGTTGAAAAATGTGAAGATCCTCTGTGACTGCAAGCATACGCGATAGTATGGCGACCAGCCACTCAGTTCTTTGTGACCAAAACGTATGGCCTCGGATCAGGTGGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCCTAGCTTGGTCTTGGGCCCTGCCGGCAACGGCTGGCCTTAAAAGCAGTGGCGGTGCCATCGAGCTCTACGCGTAGTAATTAAACCCTCGCGTGTGAGTCCCGGTGGACGCTTGCCAGCAACCCCCAATTTTTTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGTATTCCGGGGGGTATGCCTGTTCGAGCGTCATTGCAACCCTCAAGCATTGCTTGGTATTGGATGCCACCGCTGTGGTGCATCTCAAAAGTATTGGTGGTGGCATTCAGCTTCTAGCGTAGTAAATCTTCTCGCCTTGGAGTTTGAGTGTCTGACTGCTAGAACCCCTAAATTTATCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAACTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGCCCTCAAGCACGGCTTGTGTGTTGGGCTCCGTCCCCCCGGGGACGGGTCCGAAAGGCAGCGGCGGCACCGAGTCCGGTCCTCGAGCGTATGGGGCTTTGTCACCCGCTCTGTAGGCCCGGCCGGCGCCAGCCGACAACCAATCATCCTTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 4
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTATAACCAATCCAGCTCGCTGGGTCTTGGGCACCGCCGGCTGGCGGGCCCCAAAAGCAGTGGCGGTACGGCCGGGCTCTCAGCGTAGTAATACTCCTCGCTACAGATTCCCGGGCGGCACCTGCCAGAACCCCCCAATTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCATTAGTATTCTATTGGGCATGCCTGTTCGAGCGTCATTTTGACCACTTAAGCCTAGCTTAGTGTTGGGAGTCTACGTTTGTAGTTCCTTAAATCTAGTGGCGGAGCCGCAGTGGCCTCTGAGCGTAGTAATCTTTTCTCGCTTCTGAAAATACTGTAGTTTTTGCCATTAAACCCCCAATTTTTTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCCTGGCTTGGTATTGGGCTTCGCCACTAACCTGGCGGGCCTTAAAATCAGTGGCGGTGCCTCCGAGGCCCTGAGCGCAGTAAATATCCTCGCTATAGGGACCCGGTGGTTACTGGCCAGCAACCCCAACTTTCTAAGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 1
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGTGTCATTAAATTCTCAACTTCAAGATCTTTATTGATTTTTGAAGCTTGGACTTTGGAGGTTATTGCAGGCTTACTTTGTCAGCTCCTCTTAAATGCATTAGCGGAACTTTTTTAAGGACCAGCTTTGGTTTGATAATCCTATCTATGCCGTCGCTGTGGACTTGTATGTTCAGCTTCTAATGGTCTTTGGACTGTCTCCTTAGTGAGGCATACACTTTTAAGCTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 1
## S3 0
## S4 0
## S5 3
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCATTAGTATTCTAGTGGGCATGCCTGTTCGAGCGTCATTTCAACCCTTAAGCCTAGCTTAGTGTTGGGAATCTACTGTATTGTAGTTCCTGAAATACAACGGCGGATCTGTAATATCCTCTGAGCGTAGTAATTTTTTTCTCGCTTTGGTTAGGTGTTGCAGCTCTCAGCCGCTAAACCCCCCAATTTTAATGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAACCATCAAGCCTGGCTTGTCGTTGGACCTCTTTGCCAATGAAATATGTGGCAGGTCCGAAAGATAATGACGGCGTCGTGTTTGACCCTAGATGCAACGAGCTTTTTATAGCACGCATTGATGTGGTCGGGCGACCCAGTCTTTAACCATTATTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 8
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTGTGGTATTCCGCAGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCCTGGCTTGGTGTTGGGCCTGGCCTATCCGGCCGGCCCTAACGACAGTGGCGGCGCCGCCTGGCCCTCAGCGTAGTACAGCTCTCGCTCTAGGGTCCTGCGGTAGCCTGCCAGAACCCCCAACTCTGTGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 4
## S2 0
## S3 0
## S4 0
## S5 4
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTGTGGTATTCCGCAGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCCCTGCTTGGTGTTGGGCCCTGCCCGTTGCGGCCGGCCCTAAAGTCAGTGGCGGCGCCGTCTGGCTCTAAGCGTAGTACATCTCTCGCTCTAGGGCCCCGCGGTGGCTTGCCAGCAACCCCAACTCCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 2
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTGTGGTATTCCGCAGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCACTGCTTGGTGTTGGGCCCTGCCCGTCGCGGCCGGCCCTAAAGACAGTGGCGGTGCCGTCTGGCTCTAAGCGTAGTACAACTCTCGCTCTAGAGTCCTGCGGTAGCTCGCCAGAACCCCTAATCTTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATTGAATCTTTGAACGCATCTTGCGCCTCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCGTTAGAACTATCAAAAAACAATGGTTTCGATCATTATTTTTTGGAATTGGAGGTGGTGCCCTGGGGTCTTTTGAGAAAAAGACTCTCCGAGCTCCTCTGAAATGCATTAGCGAAATGCAGTGCACTTTCTTCTCCTTGGGGCATTGATAGTTACTTTCATGTCCTAAGCTGCGGGGAGGAAGTCACAGAATGCTTTCTTGCTCCTGAATGTGTAAATCTTGACAATCAAAGCCCTCAAATCAGGCAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCTCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTAAAATAAACTTCAAGCACTTTATTGTGCCTTGGTCTTGAGGGATGAGCAGGTAAGATTATATCTAATTGGCTCACCCATCGAATTCTAGTGGCGGAATCAAATACGGATTGTCTTGGTGTGATAAGTTTTTTCACGTCACCAATAAGCTCGTATTATATTCTAGCCAATTCAAACTCCCTCTCTCTTTAATCAACGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACTTGGTGTGAATTGTAGAATCCCGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCGACGCCATTCGGCCGAGGGCACGTCTGCCTGGGTGTCACGCATCGTTGCCCCCAAACGCCCCCGCCTCGCAAGGGGCGCGGGATCTCGTTTGGTGGCGGAAGTTGGCCTCCCGTGGGCCTGTGCTCGCGGTTAGCCTAAAAAGGAGTCCTCGGCGACGAGCGCCACGACAATCGGTGGTTGATGAGACCTCGGTCCCCGTCGTGCGTGTCTGGTCGCCACACGGTGTGACTCGTCGACCCTAACGCGTCGTACCCACGTCGCTCCCGACGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCATTAGTATTCTATTGGGCATGCCTGTTCGAGCGTCATTTTGACCACTTAAGCCTAGCTTAGTGTTGGGAATCTACGTTTGTAGTTCCTTAAATCTAGTGGCGGAGCCGCAGTGGCCTCTGAGCGTAGTAATTTTTTCTCGCTTTGGAAGGTTCTGTAGTTTTTGCCATAAAACCCCCAATTTTTTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCTACGAGTCATCGAATCTTTGAACGCACATTGCGCCTATTAGTATTCTAATAGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAGCCCTGGCGGCTTGGTGTTGGGGCGCTACAGCCTGTGCTGTAGCCCCCGAAATATAGTGGCGGACCAGACGAGGACCCGAGCGCAGTAGTACCCCTCTCGCTTAGGGCGCCCCGGAAGGACTCCTGCCGTAAAACCCCCACTGTAACCATATCTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTGTGGTATTCCGCGGGGCATGCCTGTTCGAGCGTCATTGTGACCAAATCACGCCTGGCGTGGCCTTGGGGCTGGCCGTCGAGCCTCCCCTAAACCCAGTGGCGGTGCCGTGCGGCTCTAAGCGTAGTAAAGATCCTCGCTATAGTCCCCGTGCGGTTGCTGGCCATCAACCCCCCCTACTTTCTACGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 2
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACCTTGCGCCTCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTGATTGAATCCTCAAAAGAGAATCTTCACAGATTTTCTCTTTTGGAATTGGGGTGTGCTGGCCTTTTGTTTGGAAAGCCCAGCTCCCCTGAAATACATTAGCGATGTGACATCCGCATGGCACCACGCCTCTCTGGCATTGATAGTCTTTTCATGCTTGAGCGTGTTGTGCAGCTGTGGAAAGTTTTGCTTGCTCACAACTGATGGGGGGTTTCCCCCATCCTTATGACCAATTTAACCTCAAATCAGGTAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 1
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCATGGGGCATGCCTGTTCGAGCGTCATTTACACCCTCAAGCTCTGCTTGGTGTTGGGCGTCTGTCCCCGCCTTCGCGCGCGGACTCGCCCCAAAGGTATTGGCAGCGGTCTTGCCAGCTTCTCGCGCAGCACATTGCGCTTCTCGAGGCTCCGGCGGGTCGGCGTCCATCAAGCCCACATCACAGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 2
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCACGCCTGTTCGAGCGCCATTTCAACCCTCAAGCCCTGCTTGGTGATGGGCCCCGCCCGCGGGCGGGCCCGAAACTCGTGGGCGCCGTCGCCCGGCCCCGAGCGTAGCAAAACCCTCGCTCGGGTGCCCGGACGGAGGCCGCCCGGAACGAAATTACACAAGGTTGGCCTCGGATCAGGCGGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATTTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATACCTGTTCGAGCGTCATTAACAACCCCTCAAGCCTAGCTTGGTATTGGAGTCGCTATTTTCGCGGCCCCTAAATTCAGTGGCGGTGCCTGTAGGTTCCACGCGAAGTAATTCTTCTCGCGATGGATCCCCTATAAGTCTCTAGCCAACAACCCCAATTTTTTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 2
## S2 0
## S3 0
## S4 0
## S5 4
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATAACCAATCAAGCCTGGCTTGGTGTTGGGGTTCGCGGTTTCGCGGCCCTTAAAATCAGTGGCGGTGCCGCCTGGCTCTAAGCGTAGTAACTTTCTCGCTCTTGAGTCCGGTGGTTGCTTGCCAGAATCTCCATCTCACGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCTGGAATTCCAGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCCTGGCTTGGTGTTGGGGCACTACCTGTAAAACGGTAGGCCCTGAAATTTAGTGGCGGGCTCGCTAAGACTCTGAGCGTAGTAGTTTACCACCTCGCTTTGGAAGAATTAGCGGTGCTCTTGCCGTAAAACCCCCAATTTCTGAAAATTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCACCGCTTGGTCTTGGGCCGCACCGGCAACGGTGGGCCTTAAAAACAGTGGCGGCGCCATCGTGCTCTCAGCGTAGTAATTCTTCTCGCTGTTGGGTTCCGGTGGACGCTGGCCAGCAACCCCTAATTTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCAGCTTGGTATTGGGCTCGCCCTTCATGGGCCTGCCTCAAAATCAGTGGCGGCTCCGTCCAGTCTCAAGCGCAGTAATACTCGTCGCTTGTTAGGCTTGGTCGGCGGCCTGCTAACAACCCCCAATTTTACAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCACTTCAACCCTCAAGCTCTGCTTGGTATTGGGCCGCACCGGCAACGGTTGGCCTTAAAATCAGTGGCGGCGCCGCTGGGCCCTGAGCGTAGTAATACTCCTCGCTACTGGGTCCCGGTGGATGCTTGCCATCAACCCCCAATTTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 2
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATCTTGCGCCCTTTGGCTATTCTGAAGGGCATGCCTGTTTGAGAGTCATTATTATTCTCAAACTTCAGAGTTTTTGTGGACTCTGAAGCTTTGGATATGGAGGACTATTGCTGGCAAATTTGTGTTGCTGGCTCCTCTCAAATGCATTAGCGGAACTGTCAATGTCTTGGCTCTGGTTTGATACATATCTATGCCATTGCTGAGGAGCTTTCATGGTTCAGCTTATAAACCCCCCCTTTGTCTCCGAAAACTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTGGGCCTCGCTAGCAATAGCGTGCCTTAAAATCAGTGGCGGTGCCGTCTGGCTCTAAGCGTAGTAATTCTTCTCGCTATGGATGTCAGATGGATACTTGCCATCAACCCCCAATTTTTAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCAAGCTCTGCTTGGTATTGGGCTACGCCATCGCGGCAAGCCTTAAAATTAGTGGCGGTGCCGTCTCGGCTCTAAGCGTAGTAACAATCTCTCGCTCTGGAGACCTGGCGGTGCTTGCCAGACAACCACCAATTTTTTTTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 26
## S4 0
## S5 0
## S6 0
## GCGCGATAGGTATTGTGAATTGCAGAATTGTGAATCATCGAATTTTTGAACGCACATTGCACCCATTGGTATTCCGATGGGTATACTTGTTTGAGCGTCATTTCATTCTCCTTTTTGGGGTTTTAGCTTGAATAAATCTCGCTGAATTATAGTGGTGTGGCTACCAGACTACAACGTGATAGATATTTCGTTGGATGTGACTGGGATTGCTCACCTTAAAACAATTGTATAGACCTCAAATCAAGCAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCAGCTTGGTATTGGGCTCGCCCTTCATGGGCCTGCCTCAAAATCAGTGGCGGCTCCGTCCAGTCTCAAGCGCAGTAATACTCGTCGCTTGTTAGGCTTTGGTCGATGGCCTGCTAACAACCCCCAATTTTACAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACTCCTTGGTATTCCGAGGAGTATGCCTGTTTCAGTATCATGAGCACTCTCACAACTAACCTTTGGGTGAAATTGTGGAATTGGAATGCGCTGATTGTAATAACCAGCCCTTCTAAAATATATTTCATGACTGTCACCTAACACAGCAGTTTGGCCTAATAGTTTTGACATTGATTTGTCAAATCTTTGGCTTACATTTGCTTCAGGTTGTCAGTTTTGATAATACAGAAAACTCATTCAAATTTTGATCTGAAATCAGGTAGGGCTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACCTTGCGCCTCTTGGTATTCCAAGGGGCATGCCTGTTTGAGTGTCATTGAATCCTCAAAAGAGAGAGACTTAACTTTTTTCTCTTTTGGAATTGGGGTGTGCTGGCCTTTTGTTAGGAAGCCCAGCTCCCCTGAAATACATTAGCGATGTGACATCCGCATGGCACCACGCTTCTCCGGCATTGATAGTCTTTTCATGCTTGAGCGTGTTGTGCAGCTGTGGAAAGTTTTGCTCGCTCACAACTGATGGGGGGGTTTTCCCCCCATCCTCTGACCAATTTAACCTCAAATCAGGTAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCAAGCACAGCTTGGTCTTGGGCTTCGCTGCTCACCCAGCGGGTCTTAAAATCAGTGGCGGTGCCGTCGGGCCCTGAGCGTAGTACATCTCCTCGCTATAGGGCCCCGGCGGGTGCTGGCCATCAACCCCCAATTTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCTGAGGGGCATGCCTGTTTGAGTGTCATTGTAATCTCTCAACTTTGGTCTTTGGTTTCTTAACCCAAGGCCCAGAGTTTGGATGGTGGAGGTGTGCTGGCCGCTCCCCGTGGAGGGTCAGCTCCTCTGAAAAGAATCAGCAAAGGGAAACGTACCTGTTCCTGCATCTCGTGCTTCTGGCATTGATAATTGTCTATGCCTGTTGTGGCTCAGATGCCTTTTGGATCGGGCGAGCGTCTTTGCTTACAGTTGTCCTTTGGACATTTTTGACAAATCACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 1
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATCGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGCACTCAGGGGGTATGCCTGTCCGAGCGTCGTCACGACCCTCGAGCTCCCCGGAGCTCGGCGTTGGGTGCGGCCCCCGATCACTGGGGCCCTCCCCAAAACCAGTGGCGGCCGTGGCCCGGTCCCGAGCGCAGTAGACTTCTCTCGCTCAGGGGCCTGGGCGGCGTGCCGGCCAGCAAGACTTCAGCCACACCAAAGGTTGACCTCGGATCAGGTAGGAGCACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTAAATTCTCAACCTCTTCATTTTGTTTTACAACTTTGAATTGGCTTGGATATGGGAGTTGCGGGCTTCTAAGCAAGTCAGCTCTTCTTAAATGCATTAGCAGAACTTTTGTTGACCATTATTGGTGTGATAATTATCTACGCCATTTGATGTGAAGCAGTTTATATAGAGTTCAGCTTCTAACTGTCTATTCACTTGGACAATTTATTGACTATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCATTAGTATTCTATTGGGCATGCCTGTTCGAGCGTCATTTCAACCCTTAAGCTCAGCTTAGTGTTGGGAATCTACCGTGAGCGGTGCTACCTGGTACCTACCCTGTAACGGGGCTAGGTTACCCTGTAGTTACCCTGTAGTTCCTCAAATCCAACGGCGGAGTCATAGTCATCTCTGAGCGCAGTAATTTTTATCACGCTTTTGAAGATGCTATAACCTTAGCCGCTAAACCCCCAATTTTTTAATGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATATTGCACCTTCTGGTATTCTGGGAGGTATGCCTGTTCGAGCGTCATTACGACCAACAAGCCCTTTAAGCTTGGTGTTGGATGCCACTATATATTAGGGTGCATCTTAAAATCAATAAGCAGTTTTCTCGGCTTCCGAGCGTAGTAATTTATTTCGCTTTTGGTGCTTAGATGTCCTGCTAAATACTTAAACATATCATGGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGACCTCAGTGAATCATCGAATCTTTGAACGCATATTGCACTCTCTGGTATTCCGGAGAGTATGCCTGTTTCAGTATCAGTAAACACCCTCAAACACCTCCAAGTGGGAGGGGTTTGGATTTGAGCGTTGCCGAGAAAACTTTCTTTTTCGGCCTGCTCGAAATGCAAGTACACAGGACAGGATCCCAGACCACCCAGGTGTGAAAAAAAAGCGTAGTAGTTGACTTTATTGTCTTCTTTCGCTGAAAAAGAACGCCCGGTTTTTTTTTGGGGACCTGCTGTGCAAATTCGCAGGAAGAAAAGGAATCTCCTTTTCACTTCTTGATCTGAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 4
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTGCAACCCTCAAGCTATGCTTGGTATTGGGCTCCGTCTACTACTGACGGGCCTTAAAATCAGTGGCGGTGCCATTAGGCTCTAAGCGTAGTAATTCTCTCGCTATAGACGTCTGGTGGATACTTGCCAGTAACCCCCAATTTTTAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTTAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGTATTACTTGGTATTGGGCTCTACTGCTTACCCAGTGGGCCTTAAAATCAGTGACGGTGTCGTTGGGGCCCTGAGCGTAGTAAATATCCTCGCTATAGGGACCTGGTAGACGCTGGTCATCAACCCCCAACTTTCTAAGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 8
## GCGCGATAGGTTTTGTGAATTGCAGAATTGTGAATCATCGAATTTTTCGAACGCACATTGCACCCATTGGTATTCCGATGGCTACACTTGTTTGAGCGTCATTTCATTCTCCTATATGGGTTTTTAGCTTGATTAAATCTCGCTGAATTATAGTGGTGTCGCTACCAGACCACAACGTGATAGATATTTCGTTGGATGTGACTGGGATTGCTCACCTTAAATATTATGTATAGATCTCAAATCAAGCAGGACTACCCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGATTTTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTAAAGTCATCAACCCCGGGTCGATTTGTTTCGGTTCGGGGCTTGGAATTGGAGCGTGCTGGCGAAGGTCGGCTCCTCTCAAATGCATCAGCGGAAGTATTACAACATCTTTGAATTCCGGAACGTCGGTGTGATAATCGCGTTGCGTCGCGGTCCGGGTTCGGAGTTCCGCTCACAATTGTCTTCGGACGCGTCATCATCGAATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTTTAGTATTCTGAAGGGCATGCCTGTTCGAGCGTCATTATCACTAATCAAGCCCGGCTTGCTGTTGGACCTCTTGTCGTTAAAAGACAGGTCCCAAAGATAATGACGGTGTTATTAGAACCCTAGATGCAACGAGCTTTAACCAGCACGCATGATAGTGGTCTTTTAACCCGGTCTTTTTTAACCAATTTTTTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTGGGCGTCACCCCCGGGTGCGCCTCAAAAACAGTGGCGGTGCCGTCTGGCTTCAAGCGTAGTAATTTCTCTCGCTCCGGAGTCCAGGCGAGTGCTCGCCAGAACCCCCAATTTATCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTGGGCATCACTAGAAATAGTGTGCCTTAAAGACAGTGGCGGTGCCATCTGGCTCTAAGCGTAGTAATTCTTCTCGCTATAGATGTCTTGTGGATGCTTGCCAGCAACCCCTAATTTTTTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCACGCCTAGCGTGGTATTGGGCTCGCCCTGTAGGGCTTGCCTCAAAGTCAGTGGCGGCGCCGTTTGACCCCTAAGCGTAGTAATACTCGTCGCTTCTGGTGTTGTACGGAGGCCTGCCAGCAACCCCCAATTTTTAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGATCTCTCAGTGAATCATCGAATCTTTGAACGCACATAGCGCCCGCCGGCTATTCCGGCGGGCATGCCTGTCCGAGCGTCGATGACGCCTTTCGAGCCCCTCTGATGGGCTCGGTGTTGGGGCGCTAGGGGGCGGTTTCTCGCGAAGCCCCCCCTAGGCCCCCAAGCAGAAGTGCGCGGTCCGTCTGCGGGCCCCGGGTACAGTAACTTTACCGTTAGATGCCCTGGGAGAAGCAGCTGGGCTTGCCGTAAAACCCCCGACATAGGGACCTCCGTGCCTCGCGCAGGGAGGGACCGCCATATGTTGACCTCGGATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 3
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGTATTCCGGGGGGTATGCCTGTTCGAGCGTCATTGCAACCCTCAAGCACTGCTTGGCATTGGATGCTACCTCTTGGTACACCTCAAAAACATTGGCAGTGGCATTCAGCTTCTAATGTAGTAATTTTCTCGCTCAGGAGTCATGAATGTCCAACTTGCCAGAACCCTCTAATTTATCACAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 3
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACACCACTCAAGCTCTGCTTGGTATTAGGTCTCACCTGCTAAAGGCGGGCCGTAAAATCAGTGGCGGTGCCATCTGGCTTCAAGCTTAGTAATTTTTCTAGCTTAGGAGTACCAGGTGTGTGCCTGCCAACAACCCCAATTTTTTTTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATCTTGCGCCCTTTGGCTATTCTGAAGGGCATGCCTGTTTGAGAGTCATTATTATTCTCAAACTTCAGAGTTTTTGTGGACTCTGAAGCTTTGGATATGGAGGACTATTGCTGGCAAATTTGTGTTGCTGGCTCCTCTCAAATGCATTAGCGGAACTGTCAATGTCTTGGCTCTGGTTTGATACATATCTATGCCATTGCTGAGGAGCTTTCATGGTTCAGCTTATAAAACCCCCCTTTGTCTCCGAAAACTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCAATGCTTGGTGTTGGGCCGCGCCGCTAACCCGGCGGGCCCTAAAACCAGTGGCGGTGCCGTCGGGCTCTGAGCGTAGTAATTCTTCTCGCTATAGAGCCCCGGCGGATGCTAGCCAGCAACCCCCAATTTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 3
## S2 10
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATTTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATACCTGTTCGAGCGTCATTAACAACCCCTCAAGCCTAGCTTGGTCTTGGAGTCGCTATTTTAGCGGCCCCTAAATTCAGTGGCGGTGCCTGTAGGTTCCACGCGAAGTAATTATTCTCGCGATGGATCCCCTATAAGTCTCTAGCCAACAACCCCAATTTTTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 5
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAATTAATGTGAATTGCAGAATTTAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCACTGCTTGGTCTTGGGCCGCGCCGGTAACGGCGGGCCTCAAACGTAGTGGCGGCGCCATTGGTTCCCTCAGTGTAGTAACATCTCTCGCTGTTGGGCCCCGGTGGTCGCTAGCCAGCAAACCCCAACTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCACGCGTAGCGTGGTCTTGGGCTCTGCCGGCAACGGCTGGCCTTAAAATCAGTGGCGGTGCCATCGAGCTCTACGCGTAGTAATAAACCCCTCGCGTGTGAGTCTCGGCGGTCGCTTGCCAGCAACCCCCAATTTTTTATGGTTGACCTCGAATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCCGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCGCCAGCATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAGGCCCTGCCTGGCGTTGGAGGACTGCGCACGCAGCCTCCCAAAGCGAGCGGCGGCGGCGCCCCGAAACCGAACGCAGTAGTTACTTCTCGTTCTGGGTCCGGGTCGTTGTCCTGCCGGAAAACCCCCCTCTAATCAATGGTTGACCTCGAATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 1
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTATACCAATCTAGCCTGGCTAGGTGTTGGGCTTCGCCGTCTGGCGGGCCTTAAAACTAGTGGCGGTGCTCTTAGGCTCTACGCGTAGTAATTTTCTCGCTATAGGGTCCTGGGAGATGCTGGCCAGCAACCCCAAATTTTTCTAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGATTTTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTAAACCTTCAACCCTGATTTGAATTTTTTCTACTCGGGAGTTTGGACTTGGAGCGTGCTGGCGCGAGTCGGCTCCTCTGAAATGCATCAGCGGAAACCAAATCCTTGGCTTTCTGGATGTCAGTGTGATAATTGTGTTGCGCTGTCGTTCGAAGCTTTAAGGTCCGCTTACAATGGTCTTCGGACGATTCATCTCACTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCAACTTGCGCTCCTTGGTATTCCATGGAGCATGCCCGTTTGAGTGTCACGATAACCCTCATTCATGCGATTTTAATGATTGCATGAATGGATTTGGACGCTGCCGTAAATGGCTCGTCTTGAAATGCATTAGCTTTGCGCCCATTAGCGTGCTCGGTGTGATAATTATCATTCGCCTCAGGCCGCTTCCAGTGACGCTGCTTCTAATGGTCCTTTGCGGACGATCAATCTAAAGCTTTGGCCTCAAATCGGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTAGGCCTTACCTGCTACAGGCAGGCCGTAAAATCAGTGGCGGTGCCATTCGGCTTCAAGCGTAGTAATTCTTCTCGCTTCGGAGTACCGGTTGCGTGCCTGCTATAAAACCCCAATTTATCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCCGTTCGAGCGTCATTATAACCCCTCAAGCTCAGCTTGGTGTTGGGGCCTGCTGTTACCGGCAGCCCTTAAAATCAGTGGCGGTGCCATCTGGCTCTAAGCGTAGTAACACTTCTCGCTACAGGGTCCCGGTGGATGCTTGCTATCAACCCCTCAACTTCTATGGTTGACCTCGGATCGGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 21
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAACCATCAAGCCTGGCTTGTCGTTGGACCCCCTTTTGCCACTGAAATATGTGGTAGGTCCGAAAGATAATGACGGTGTCGTGTTTGACCCTAGATGCAACGAGCTTTTAATAGCACGCATTGAAGTGGTCGGCCGACCCAGTCTTAAACCATTATTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 9
## S4 0
## S5 1
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAACCATCAAGCCTGGCTTGTCGTTGGACCCTTTTGCTGCTGAAATATGTGGTAGGTCCGAAAGATAATGACGGTGTCGTGTTTGACCCTAGATGCAACGAGCTTTTAATAGCACGCATTGAAGTGGTCGGCCGACCCGGTCTTAAACCCATTATTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 3
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACTCCTTGGTATTCCGAGGAGTATGCCTGTTTCAGTATCATGAGCACTCTCACACCTAACCTTTGGGTTATGTCGTGGAATTGGGATGCGCCGATTTTTATTAGTCGGCACTCCTAAAATGTAGCTCTTGGCTGTTTCCTACTACAGCAGTTTGGCCTAATAGTTTTGACTTTTGTCAAATCTTTGGCTACATTTGCTTCTGGAAATCAGTCTTGATAATACAGAAAACTCATTTCAAACTTTGATCTGAAATCAGGTAGGGCTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCACACCTGTTCGAGCGCCATTACAACCCTCAAGCCTTGCTTGGTGATGGGCCCCGCCTGCGCGGCGGGCCCGAAACTCGTGGGCGCCCCAGACCGGACTCCAAGCGTAGCAAAACCCTCGCTTGCGGATGACCGGTAGGAGGCCGCCCCGGACCCCAAAATTTTTATCAATGGTTGGCCTCGGATCAGGTGGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGTATGCCTGTTTGAGTGTCATTATCACCTCAAAGCAATAGTGGGTTAATTCCTTCTTTTGCAATTGGATGTTGAAGGTTTTGCTGGTGCCCTTGATGCAGTTCAGCTCCTTTTAAATGCATTAGCAGGACTTTGCATGACCAGCTCCAGTTTGATACGACAGTTATCTGAGCTGCTGCTGTGAAAGCTCTTAGTCATCTGCTTCTTGAGCAGTCTGTGGACTTTGTCCCAGTGGACAAACAAACATACTTTGAACTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTACAACCCTCAAGCTCAGCTTGGTATTGGGCCCCGCCGACCCGGCGGGCCCTAAAGTCAGTGGCGGTGCCGTCCGGCTCCGAGCGTAGTAATTCTTCTCGCTCTGGAGGCCCGGTCGTGTGCTCGCCAGCAACCCCCAATTTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCAGCTTGGTATTGGGCTCGCCCTTCATGGGCCTGCCTCAAAATCAGTGGCGGCTCCGTCCAGTCTCAAGCGCAGTAATACTCGTCGCTTGTTAGGCTTTGGTCGGTGGCCTGCTAACAACCCCCAATTTTACAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGTATGCCTGTTTGAGTGTCATTATCACCTCAAAGCAATAGTGGGTTAATTCCTTCTTTTGCAATTGGATGTTGAAGGTTTTGCTGGTGCCCTTGATGCAGTTCAGCTCCTTTTAAATGCATTAGCAGGACTTTGCATGACCAGCTCCAGTTTGATACGACAGTTATCTGAGCTGCTGCTGTGAAAGCTCTTGGTCATCTGCTTCTTGAGCAGTCTGTGGACTTTGTCCCAGTGGACAAACATACTTTGAACTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGTCCCCTGTGGACTCGGTGTTGGGGACCGGCGAGACAGCCGCGGATCTTCTTCCGCGGCGAGTCGCCGCCCCCTAAATGACTTGGCGGCCTCGTCGCGGCCCTCCTCTGCGTAGTATAGCACACCTCGCAACAGGAGCCCGGCGGATGGCCACTGCCGTAAAACCCCCAACTTTTTCAGAGTTGACCTCGAATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 9
## S4 0
## S5 1
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCACAGCTTGGTATTGGGCTCCGCTGGTTCCAGCGGGCCTTAAACTCAGTGGCGGCGCCCTCAGGCTCTGAGCGTAGTAAACATTCCTCGCTACGGAGTCCTGGGCGGCGGTGGCCATCAACCCCCAACTTTCTAAGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTTTAGTATTCTGAAGGGCATGCCTGTTCGAGCGTCATTATCACTAATCAAGCTCGGCTTGCTGTTGGACCTCTTGTCGTTAAAGACAGGCCCCAAAGATAATGACGGTGTTATTAGAACCCTATATGCAACGAGCTTTAATCAGCACGCATGTTAGTGGCCTTTTAACCCGGTCTTGCTTTACCAATTTTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATTGAATCTTTGAACGCATCTTGCGCCTCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTAGAACTATCAAAAAACAATGGTTTCGATCATTATTTTTTGGAATTGGAGGTGGTGCCTGGGTCTTTTGAGAAAAAGACTCCCCGAGCTCCTCTGAAATGCATTAGCGAAATGCAGTGCACTTTCTTCTCCTTGGGGCATTGATAGTTACTTTCATGTCCTAAGCTGCGGGGAGGAAGTCACAGAATGCTTTCTTGCTCCTGAACTTGTAAATCTTGACAATCAAAGCCCTCAAATCAGGCAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAACCATCAAGCCTGGCTTGTCATTGGACCCCTTTGCGGCTGAAATATGCGGCAGGTCCGAAAGATAATGACGGCGTCGTGTTTGACCCTAGATGCAACGAGCTTTTCATAGCACGCATTGAAGTGGTCGGCCGACCCGGTCTTTAACCATCATTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTAAATTCTCAACCTTACTAGCTTTTGTTGGTAATGGCTTGGATGTGGGGGTCTTTTGCTGGCTTCGTGAAGAGGTCTGCTCCCCTTAAATGCATTAGCCGGTGCCCTGCGTGGATCCGTCTATTGGTGTGATAATTATCTACGCCGTGGACGTCTGCTTTAAATGGGATGCACTGCTTCTAACCGTCCGTTCAGTTGGACAATCAATGACAATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 2
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCATCAAGCTCAGCTTGGTATTGGGCTCGCCCTTCATGGGTCTGCCTCAAAATCAGTGGCGGCTCCGTCCAGTCTCAAGCGCAGTAATACTCGTCGCTTGTTAGGCTTGGTCGGCGGCCTGCTAACAACCCCCAATTTTTACAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCATTAGTATTCTATTGGGCATGCCTGTTCGAGCGTCATTTCAACCCTTAAGCTCAGCTTAGTGTTGGGAATCTACCGTGAGCGGTGTTACCCGGTACCAACCCATTTCGGGGAGGTTACCCTGTATCTACCCTGTAGTTCCTCAAATCCAACGGCGGAGTCATAGTCATCTCTGAGCGCAGTAATTTTTATCACGCTTTTGAAGATGCTATAGCCCTTGCCGCTAAACCCCCAATTTTTTAATGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTTAGTGAATCATCGAATTTTTGAACGCACATTGCGCCCTCTAGTATTCTGGGGGGCATGCCTGTTCGAGCGTCATTACAATCCTCAAGCACTGCTTGGCATTGGGCATCACCTGTCTAGGTGTGCTTTAAAATCAGCAGTGGTGCCATCTGACTTCAAGCGTAGTAAATTTTCTCGCTTTGGGGACTAGATGAGTGCCAACCAACAGCCTTTAAACCTTTAAAAATTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATCCCGAGGGGCATGCCTGTTCGAGCGTCATTTCACCACTCAAGCCTGGCTTGGTGTTGGGCGACGTCCCCTTCGGGGACGCGCCTCGAAACGCTCGGCGGCGTGGCACCGGCTTTAAGCGTAGCAGAATCTTTCGCTTCGAAAGTCGGGGCCCCGTTTGCCGGAAGACCTACTTGCAAGGTTGACCTCGGATCAGGCAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTGAATTCTCAAAATTCTGAACCTCGCGGGTTCAGGCTTTTGGATATGGAGAGTGCCGGCCTTCAACCGAGTTCGGCTCCTCTGAAAAGCATTAGCAAAACCCTCTCCCCGATGGCTTTGTGGCATTGATAAGACTGTCCATGCCTTTGCCCTCCGGCGGAAAGCGGTTGCTTTGACAAACCTTGACCATTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTCGGTATTCCGTTGGGCATGCCTGTTCGAGCGTCATTTAAACCTTCAAGCTCTGCTTGGTGTTGGGTGTTTGTTCCGCCTCTGTGCGTGGACTCGCCTTAAATTCATTGGCAGCCGGTATGTTGGCTTCGCGCGCAGCACATTGCTAGCGAGTCCATTGTGCCCCCTTCCATTAAGCCTCTTTTTTACTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCAACTTGCGCCCCTTGGTATTCCGAGGAGCACGCCTGTTTGAGTGTCGTGAATTCTTCCATCATTCAATTTTTTGAATGACTGGGATTTGGGGGTTTGCGGGATCCTTTACGGAATCCGCTCCCCTTGAATGCATGAGTGGAGACCCTTGCGGGCCTTGGTGTGATAATGTCTACACTTTGGTTTGTCGAGATCAAGGCCTTTGCTTCCAACCGTCCGTTCCACGGACAAATTTTTAAACTTGACCTCAAATCAGGCGGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGTATTCCGGGGGGTATGCCTGTTCGAGCGTCATTGCAACCCTCAAGCACTGCTTGGCATTGGATGCTACCTCTTGGTACACCTCAAAAACATTGGCAGTGGCATCCAGCTTCTAGTGTAGTAATTTTCTCGCTCAGGAGTCATGAATGTCCAACTTGCCAGAACCCTCTAATTTATCACAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCCCCAGTGGCCTGGTGTTGGGGACCGGCGACCTCGCCCGGGGGAACACCCCCGGCGCGTCCGCCGCCCCCGAAATGAATTGGCGGCCCCGTCGCGGCCTCCTCTGCGTAGTAGCACAACCTCGCAGCGGGAGCGCGACGCGGCCACTGCCGTAAAACGCCCAACTTTTTTTAGAGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 3
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGGTCGGCGTTGGGGATCGGCCCTTCACGGGGCCGGCCCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACACTCGCATCGGGAGCGCGGCGCGTCCATTGCCGTAAAACACCCAACTTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 1
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATAACCACTCAAGCCTTCGCTTGGTATTGGGGCTCGCGGTCGCGCGGCTCCTAAAATCAGTGGCGGTGCCGATTGGCTCTACGCGTAGTAATACTCCTCGCGTCTGGGTCCGGTCGGTTGCCTGCCAGCAACCCCCCAATTTTTACAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGAATTCCGAGGGGCATGCCTGTTCGAGCGTCATGATCAAATCTCAAGCCTTGCTTGGTATTGGGCTTTCGTCTCTCCTGCTTGTGTGCAGGTAAGTGGCGTGCCTGAAAGTCACTAGCAGTGCCTCAATAGACTCAAGCGTAGCAGACTGTACTCGCTTTGGAGGCCCTTTGTAGACACTGTTAACAAAACAATCATGTTGGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 1
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTATACCAATCTAGCCTGGCTAGGTGTTGGGCTTCGCCAGCTGGCGGGCCTTAAAACTAGTGGCGGTGCTCTTCAGCTCTACGCGTAATAATTTTCTCGCTATAGGGTCTGGGGAGATGCTGGCCAGCAACCCCAAATTTTTCTAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 1
## S6 9
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCTCCTGGTATTCCGGGAGGCATGCCTGTTCGAGCGTCATCAAACACATCCTCAAGCATCTTTGCTTGGTTGTGGAGGAAGATGGTCTGTTGACCATCCCCTCTAGAATTCCTCAGCGGAGACCACAGCAGCCTCAGGTGTGATAATACTACCTTGCTTGAGTGCTATTGTGGGCTCCCGCTGGAAACCAGCCCCCAGCTATTACCATGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATAACCAATCCAGCTCGCTGGGTCTTGGGCACCGCCGGTTGGCGGGCCTCAAAAGCAGTGGCGGTACGGCCGGGCTCTAAGCGTAGTAATACTCCTCGCTACAGACCCCCGGGCGGCACCTGCCAGAACCCCCCATTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCTCCTGGTATTCCGGGAGGCATGCCTGTTCGAGCGTCATTAAAAACCACTCAAGCTCTTTTGCTTGGTCATGGAAGAGGAGTATGCTAGTCATTCTCCCTTTCGAAATTCAAAGGCGGAAAGCCCCATGTGCCCCGGCGTAGTAAGTTTTCTTTCGCTTGGAACATGAGGTGATCCTGCCACAAACCCCCAATTTTTCTAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCGCCAGTATCCTGGCGGGCATGCCTGTTCGAGCGTCATTTCCACCCTCAGGCCCTGCCTGGTGTTGGGGGCCTGCGCACTGCAGCCCCCGAAATACAGCGGCGGTCCCGGCCCCGGCCCCGAGCGTAGTACTTTACGTCGCTTCGGGTGCCGGGCGGGCTCCGGCCGCAAGCCTCTATTATACAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCGCCAGCATCCTGGCGGGCATGCCTGTTCGAGCGTCATTACCACCCTCAGGCCCTGCCTGGTGTTGGGGGCCTGCGCCTGCAGCCCCCGAAAGACAGCGGCGGTCCCGGCCCCGGCCCCGAGCGTAGTACCATACGTCGCTCCGGGCGCCGGGCGGGCCCCAGCCGGAAGCCATTATTCTTCAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTTAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGATATTCCGAGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGTATTACTTGGTATTGGGCTCTACTGCTTACCCAGCGAGCCTTAAAATCAGTGGCGGTGTCGTTGGGGCCCTGAGCGTAGTAAATATCCTCGCTATAGGGACCTGGTAGACGCTGGTCATCAACCCCTAACTTTCTAAGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAAACCATCAAGCTTGGCTTGTCGTTGGGCCCTTTGTCACCTGGTGACAGGTCCAAAAGATAATGACCGGTGTCGTACAGACTCTATATGCAACGAGCTTATAACAGCACGCATCTAGTGGTCATATGGCCCGGCTCATCACCCCTTTATTTCTCAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 5
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTTGAGTGTCATGTAGACTCAATCCCTCGGGTTTCCGAGGAGATTGGACTTGGGTGTTGCCGCTCTGCCGGCTCACCTTAAAAGACTTAGCGGGATAGCACCGTAGTCGGCGTAATAAGTTTCGTCGGTGAAGGTTGTGATGACTGCTTACAATCGCCCTCGGGCAATTTTTGACTCTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 12
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGCATTCCGAGGGGCATGCCTGTTCGAGCGTCATTTAAACCAATCCAGCTTGCTGGGTCTTGGGCTTTCGCCTCTGGGCGGGCCTCAAAATCAGTGGCGGTGCTGCCAGGCTCTAAGCGTAGTAATTCTTCTCGCTACAGGGTCCTGGTGGAGGCTTGCCAATAACCCCAAATATTTCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 3
## S5 3
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCGCGAACTTCTCAACCTCGCAACGCTGCCGAGTGTTGGATCTTGGAGGTTCTGCCGGACTTTTTTTTAACGTCGGGCTCCTCTGAAACGCATCAGCAGACTAATTGTTTTTCTTTGCTGGCTTTGGTTTGATAAATCTATCTATGTCATCGCCATCTTGTTAAACGAAAAACGGTCAGCTTACAATCGTCCCTCTGGGACAATCGCTTGACGAATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 13
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATAACCACTCAAGCCTCGGCTTGGTCTTGGGGTTCGCGGTCTCGCGGCCCTTAAAATCAGTGGCGGTGCCGTCTGGCTCTAAGCGTAGTAATTCTCTCGCTATAGGGTCCAGGTGACCACCTGCCATAACTCCCCATTCTTTACGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCTCCTGGTATTCCGGGAGGCATGCCTGTTCGAGCGTCATGAAGACAACTTCAAGCTCTTTTGCTTGGTTCTGAAGGCTGAGTGTGATCCATGTACTCATTCACCTTTGAAATTTACAGGCGGACTCTTCCATGTTCCATTTGGCGTTATAAGATTACTCTTTCGCTTGGACTCGTGGGTTCTGTCCTGCCTCAAACCCCCCATTCATTTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCACACCTGTTCGAGCGCCATTTCAACCCTCAAGCCCTGCTTGGTGATGGGCCCTGCCTGCTGGCGGGCCCGAAACTCGTGGGCGTCGTCGTTCGGCTCCAAGCGTAGCAAAACCCTCGCTTGGGCGCCTGGACGGCGGCCGCCTGGAACGAATTTTTACAAGGTTGGCCTCGGATCAGGTGGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ACGCGATAGGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCTCCTGGTATTCCGGGGGGCATGTCTGTTTGAGCGTCAGTACCCACTCTTTCGAAATCTTTTTTTCGTTAGGTCTTGAAGGACACTTGTCCTTTCAATGTAGACGCTTGCGAACCCGATACCGATCATGACGTAGTAATTTCATTTCGTCTTGTCAAGTTAAAGGCCGCCTGAACACCAAACTTTGTAGTTTGACCTCAGATCAGACAAGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCACTCTTTGGTATTCCGAAGAGTATGTCTGTTTGAGTGTCATGAAACTCTCAACCCCCCTATTTTGTAATGAGATAGGCGTTTGGGCTTGGATTGTGGCTGTGTGCTGGCTTTCGAGTCGGCTCGGCTGAAATACACGAGTAACCCACTTCGATTATGAGCGGTTTGACTCGGCGTAATAGCTTTATTTCGCTGAGGACGCTTTGTTATCGAAATTGTGGTGCTTCTAATGAAACTTTCAATTAAGCTTTAAGACCTCAAATCAGTCAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGCATTCCGAGGGGCACACCCGTTTGAGTGTCGTGAAATTATCAAAACTCTTTCTTTTTGGTCTTTTGATCTGGGGAAAGGTTTTGGACTTGGAGGCCTTTTGCTGGTTTCACCTTGAAGCGAGCTCCTCCTAAATGAATTAGTGGGGTCTGCTTTGCCCGATCCTTGACGTGATAAGATGCTTCTACGTCTCGGGTTTGGCACTGTCCCTTGGGCACCTGCTTCTAACCGTCCTCATGGACATCGATGGTGTTCTGGTCACCACCGTTTCATCGGTGGGAGGCTTGACCCACGAACGAACCTTGACCTCAAATCGGGTGAGACTACCCGCTGAACTTAA
## S1 2
## S2 0
## S3 0
## S4 5
## S5 0
## S6 0
## TTGCGATAAGTAATGTGAATTGCAGATTTCCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGCATTCCGAGGAGCATGCCTGTTTGAGTGTCATTGAATTCTCAACCATGTCTTGGAGACATTACATGGCTTGGATGTTGGGGGGGTTTGCTGGCATTCCCAAGATTGTCAGCTCTCCTGAAATGCATTAGCAAAATGGCAGACATACACGGCCTTCCGACGTGATAATGATCGTCGTGGCTGGAGTGTCAGACGCCCATGTTGCTTCCAATTGAGCGTGTTTAGCTACTAGTCGGTCGTGAGGCTGGCGAACGCGGCACGACGTTGACAACTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATTTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATACCTGTTCGAGCGTCATTACACCCCTCAAACTTAGGTTTGATGTTGGGCCATTGCCCTTTGGGGCATGCCTAAAAGATAGTGACGGGCCTTAATGACCACAGGCGCACAGATGTCGCAATGTTGGGATATTAAGGTCTTGTCTAAACCCAATTTTAAATGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTTTGACCAACTCCTATTCTTTTATAGGGTCTTGGGTTATGCCTACCAGGCAGGCCTCAAAACCAGTGGCGGTGCTATATAGCTCTACGCGTAGTAATACTTCTCGCTATAGACCCTATATAGTATCTTGCCAACAACCCCCAACTTCTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATAACCACTCAAGCCTTCGCTTGGTATTGGGGCTCGCGGTCGCGCGGCCCCTAAAATCAGTGGCGGTGCCGATTGGCTCTACGCGTAGTAATACTCCTCGCGTCTGGGTCCGGTCGGTTGCCTGCCAGCAACCCCCCAATTTTTACAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGTATTCCGGAGGGTATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTCAGCTTGGTATTGGGTGTCACCTGGCAATGGTGCACCTTAAAATCAGTGGCGGTGCCATCTGGCTTCAAGCGTAGTAAAACAATCGCTTTGGAGGTTTGGATGTGTCCTTGTCATCAACTCCTAATTTATCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATATGGCACCCTCTCTTGAAATAGGAGGGTATGCCTGTTTGAGTGTCATTAAACTTCTCAATACCATTTGATTGGTGGTTGGATGTTGAAGGTCTGCTGGTAAATCCCATCATCAGCTCCTTTTAAATACATGAGCAGGACTACATGCAACCAGCTCCAATTTGATAAGCTTGTTATCTTTGTTGCTGCTGGGAGTGAATAATGTAATTGTCTGCTACTAGTTGTCCTGACCATTGGGACTTGCCTAATGGGCAACATTTGAACCCTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCTTCAAGCTCTGCTTGGTGTTGGGCCTCTGCTTGGTTACCAACCCGGCAGGCCTCAAACCCAGTGGCGGTGCCATCGGGCTCCAAGCGTAGTCGCATATCTCGCTTTGGGAGGCCCTGGTGGTTGCCAGCCAGCAAACCCCTTCTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## GTGCGAAAAGTGTTGCGATTTGCACGAATCTGTGAGTCATCTAATTTTTGAACGCGAATGGCACTGTTACGCAAGTAGCAGTATGTCTGTTTGAGAATCGCAAAACAATATCAACTCTAGTTGAGAGTTGGGATTGGCCTCATGTACCTTTGGGTGTATTTGGCTTGAATGCATCGAGTTGATGAGTTCCCCAATGCGGCTCAATGTGTGCTAGCGAGACAGTCGTCCCGATCTCACATTTCCGCCGCGTTGCAGAGTTCTACGCACAACCCGCCAACATATATTTATCAAATACGATCTCAAATCAGGCAAGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTAAATTCTCAACCTTTCTGGCTTTTATTAGCTGGTCAGGCTTGGATGTGGGGGTTGCGGGCTTCTCAGAAGTCAGCTCTCCTTAAATGCATTAGCAAAACCTTTGTTGACCATCTCTGGTGTGATAATTATCTACGCCATTGGGAAGCAGCTTTTATATGGGGTTTAGCTTCTAACTGTCTTATCATTGGACAACTCTTGACATTTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCCGTTCGAGCGTCATTATAATCCTCAAGCCTGGCTTGGTATTGGGGCCTGCTGTTACCGGCAGTCCTTAAAATCAGTGGCGGTGCCATCTGGCTCTAAGCGTAGTAATACTTCTCGCTACAGGGCCTGGTGGATGCCTGCCAACAACCCCTAATTTTTCTAAGGTTGACCTCGGATCGGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTTTGCTTGGTATTGGGAATCACCATCCTAGGTGTGCCTCAAAGTCATTGGCGGTGCCACCTGGCTTCTAGCGTAGTAATACTTCTCGCTATGGAGTTTAGGTGAGTGCTTGCCACCAACCCCTAATTTCAAGATTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCTTCTGGTATTCTGGGAGGTATGCCTGTTCGAGCGTCATTATAACCATCAAGCCTAAGCTTGGTATTGGATGCCACGTTTTTGGTGCATCTCAAAACTAGTGGCAGTGCTATTCAGCTTCAAGCGTAATAGTTTTCTCGCTTCTGGAGCTTGTTTTGTACTTGCTTAGAACCCCTAATTTAAGGCTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATGACCACTCAAGCCTAGCTTGGTATTGGGGTTCGCGGTTCCGCGGCCTCTAAAATCAGTGGCGGTGCCGTCTGGCTTCAAGCGTAGTAATTTTCTCGCTACGAAGTCCGGGTGGTTGCTTGCCAAAACTCCCCAACTTCTCAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTAAATTCTCAACCACTTCAACTTTTGTTAGTTGTGAGCGGCTTGGATGTGGGGGTTTGCGGGCTTCTTCTAGAAGTCGGCTCCCTTTAAATACATTAGCGGAACCCTTGTGGACCGTCTCTGGTGTGATAATTATCTACGCCATTGCCGGGAAACAGTTATACATGGGGATCAGCTTCTAACTGTCCCCTTGGACAAACAACTTTATTGACATTTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAACCATCAAGCCTGGCTTGTCGTTGGACCCCTTTGCGGCTGAAATACGCGGCAGATCCAAAAGATAATGACGGCGTCGTGTTTGACCCTAGATGCAACGAGCTTTTTAATAGCACGCATTGAAGTGGTCGGCCGACCCGGTCTTTAACCATCATTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCTCCTGGTATTCCGGGAGGCATGCCTGTTCGAGCGTCATTAAAGACCACTCAAGCGATTTTGCTTGGTATTGGAAGAAGAGTGCCTCTGGCCCTCCCTTCCGAAATCCAATGGCGGAAAGTCTCACGTGCCCCGGCGTAGTAAGTTTATCTTTCGCTTGGACCCTGAGGCGTTCTCGCCCTCAAATCCCCAATACTATAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 9
## S2 6
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAACCATCAAGCCTGGCTTGTCGTTGGACCTCTTTGCCAATGAAATATGTGGCAGGTCCGAAAGATAATGACGGCGTCGTGTTTGACCCTAGATGCAACGAGCTTTTTATAGCACGCATTGATGTGGTCGGCCGACCCAGTCTTTAATCATTATTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 26
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGTATTCCGGAGGGTATGCCTGTTTGAGTGTCATTTTCCACCCTCAGTATGAAATGCATTTCAACCAAATGCACCTCACACTGGATATTGAAGGTTTGCTGGTTGGCCATCTTTGGTTCTATCAGCTCCTTTTAAATGCATTAGCAGGTTTCAATGCTTGGATGACTTCAGTATAAAGTTTACTTTGAAGCCATGTGAGCCTTTACACTGGACTCTGCTTTCAAACTGTCTCCTCCTTGGGACACTTAAGAATCTTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATTTTTGAACGCACCTTGCGCTCCCTGGTATTCCGGGGAGCACGCCTGTTCGAGTGTCGTAAAATTCTCAAACTGGGATGCTTTTTTGTTTCTCGGCTTGGTGTTGGGCTTTGCAGTTCCTTTGATTGGGATAGCTGGCCTCAAAAGCATTAGCTGGCTTTTCATGTGGCATTGGTTCTACTCAGCGTGATAATGATCTGACCGCCGAGGACATCTTTCGGGATGGCCAATTCTCATTTGGGCTGCTTCTAATTTTGGTAGAGCGGATTCTGAATCTGTTCCACTTTTTCAGCTTTTGACCTCGAATCAGGTGGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGTATTCCAGGGGGTATGCCTGTTCGAGCGTCATTACAACCCTCAAGCACTGCTTGGTATTGGATGTTACCATTGTGGTATATCTCAAAAGTATTGGCAGTAGCATTCAGCTTCTAGTGTAGTAAATTTCTTGCTTTGGAGTTGGAGTGTCTAATTGCCAGAACCCCTAATTTATCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGATTTTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCCTGGTATTCCGGGGAGCATGCCTGTTTGAGTGTCATTAAATTCTCAACCCCGATCGGATTTGTTTTCGCATTCGGAGGCTTGGATAGTGGAGCGTGCTGGCCCCCTCTCATGGGTCGGCTCCTCTTAAACTCATTCGCGGAATTGACTTTGGTCCGTTTCGGTCATCAGTGTGATAAACGTTGCGCTGTTGGCTAGGATCGAGGTGCCGCATACAATTGTCTTCGGACAACTTTATGACCATTTGGCCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGTGTCATTAAATTATCAACCTTGCTCGCTTTTACCGGCTTGAGTTAGGCTTGGACTTGAGGGCTTGCTGGCTTCCTTCAGTGGATGGTCTGCTCCCTTTAAATGCATTAGTGGGATCTCTTGTGGACCGTCACTTGGTGTGATAATTATCTATGCCACTTGACCTTGAAGCAAACCTTATGGGAACCCGCTCATAACCGTCTTCGGACAACTTTTGACATTTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATCCAGTGAATCATCGAATCTTTGAACGCAACTTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTTGAGTGTCGTGAACACCTCAACCCTCTAATGTTTTTGCATTGAAGGCGTTGGTAGTGAGCGCTGCCGGTCTTTGGGTCGGCTCGCTTTAAAATAATTAGCTGGATCCATTCTGACCTGGTTCTACTCGGCGTAATAAGTTATTATCGTCGAGGACGCCCAGCCTTCTAGGCAGGCGGCCACAGTAAGGGTGTTTGAACCGCTTCCAACACGTCTTGCTCTTGTAGCAAACACTTGAACCTCTGACCTCAAATCAGGCAAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGTATTCCAGGGGGTATGCCTGTTCGAGCGTCATTACAACCCTCAAGCACTTGCTTGGTATTGGATGTCACCATTGTGGTGCATCCCAAAAGTATTAGCAGTAGCATTCAGCTTCTAGCGTAGTAAAATTTCTCGCTTTGGAGTTAAAGTGTCTAATTGCTAGAACCCCTAATTTATCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 9
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCCGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCGCCAGCATTCTGGCGGGCATGCCTGTTCGAGCGTCATTGCAACCCTCAGGCCCTGCCTGGCGTTGGAGGACTGCGCTGCAGCCTCCTAAAGCAAGCGGCGGCGGCGCCCCAAAACCGAACGCAGTAATACATCTCGTTCTGGGTCTGGGACGTTGTCCGGCCGGAAAACCCCCTCTTTTACGTGGTTGACCTCGAATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 3
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTGTGGTATTCCGCAGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTTGGCTTGGTGTTAGGCCTAGCCTATCTGGCTGGCCCTAAAGATAGTGGCGGTGCCGCCTTGCCCTCAGCGTAGTATAGCTCTCGCTCTAGGGTCCTGCGATAGCCTGCCAGAACCCCTAACTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTCGGTATTCCGTTGGGCATGCCTGTTCGAGCGTCATTTAAACCTTCAAGCTCTGCTTGGTGTTGGGTGTTTGTTCCGCCTCAGTGCGTGGACTCGCCTTAAATTCATTGGCAGCCGGTATGTTGGCTTCGTGCGCAGCACATTGCGTCACGCTTCTAGCAGACCTCCTCCCACTAAGCCTCTTTTTACTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTTCCGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTTTGGCATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCCTCTCTCAAACCCCGTGTTTGGTGTTGGACCGCGTTGGACCGCCTCCATGGCCAACTGGTCTCAAAGACAATGACGGCGTCCCAGGGACCCTCTTCGCACTGAGCTTTCCGGAGCACGCGTAGAGTTTCAAGGACCCAGGGACCCGGTCGCCTCTAAAACTTTTTTCAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTTTGGTATTCCGAAGGGCACGCCTGTTTGAGTGTCATGTTCATCTCAATCCAACAGCTTTTGTTGCTTGTCCGGATTGGATTTGGGAGCCTGCTGGCCTGACATAGAAAAAGGTCGGCTCTCCTTGAATGCATGAGTGAGCATCTGGCTCGCGTCGCTGGTGTGATAGTCATTCATCATTGGCGACGCTTGCCTAATGGGCTTGCTTTCGAACGGTCTTCTCAATGGAGACAACATTTGACACCTTTGACCTCAAATCAGGTGGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCACGGTATCAAGCCTGGCTTGTTGTTGGGCCCTTTGTCACCTGGTGACAGGTCCGAAAGACAATGACCGGTGGAACAGACTCCACATGCAACGAGCTCACAACAGCACGCATCTGGCAGTTGTGTGACCCGGTTTTACACCCCTTCTTTCTCAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTGTGGTATTCCGCAGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTCTGCTTGGTGTTGGGCCCCGCCCGCTCGCGGCCGGCCCTAAAGACAGTGGCGGCAGCGTCTGGCTCCAAGCGTAGTACAATCCTCGCTCTGGTGCTAGGCGGTGGCCTGCCAGAACCCCCCTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 9
## S2 0
## S3 0
## S4 0
## S5 1
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATTGAATCTTTGAACGCACCTTGCGCCCTTTGGCATTCCACGGGGCATGCCTGTTTGAGTGTCATTAAATTCTCAACTTCATTATTATTTTTGAAGCTTGGACTTTGGGGGTTTGCTGGTGTCATTGTCAGCTCCTCTTAAATTCATTAGCGGAACTTTGTAAGGACCGGCTTTGGTTTGATAGTGATATATCTATGCCGTCGCTGTGGACCTTTTTTTTGTTTGGCTTCTAATGGTCCATTGATATTGGACTGTCTCTCTAAGAGGCATACACTTTTAAGCTTGACCTCAAATCAGGCAGGACTACCCGCTGAACTTAA
## S1 0
## S2 2
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCACCAATCAAGCCTGGCTTGTCGTTGGACCCCTTTTGCTGCTGAAATACGTGGCAGGTCCGAAAGATAATGACGGCGTCGTGTTTGACCCTAGATGCAACGAGCTTTTCATAGCACGCATTGAAGTGGTCGGCCGGCCCAGTCTTTAACCATTATTTTCTTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 10
## S4 0
## S5 1
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGTGTCATTAAATTCTCAACTTCAAAGGTTTATTCTTTTGAAGCTTGGATGTTGGAGGACTTTTGCAGGCATATATTCAATGTCAGCTCCTCTTAAATGCATTAGCAGAACTTGTATGTTCGGCTCTGGTTTGATAATGCTATCTATGCCATTGCTGAAAGGTCATTTGTTCAGCTTCTAATGGTCCATTATGGACTGTCTCTTTGGAGGCATTATCTTTGACTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCCCCCTGTGGACTTGGTGTTGGGGACCGGCGACAATCACCTGTCGCCGCCCCCTAAATGAATTGGCGGCCCCGTCGCGGCCCTCCTCTGCGTAGTAGCATACACCTCGCAACAGGAGCCCGTCGGTGGCCACTGCCGTAAAACACCCCAAACTTTTTTAGAGTTGACCTCGAATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTGGGCTTCATCCGTCAGGGTGGGCCTCAAAATCAGTGGCGGTGCCATTCGGCTTCAAGCGTAGTAATTTTCTCGCTCTGGAGTACCGTTTGTGTGCCTGCCAGCAAACCCCAATTTTTTAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCTTTTGGTATTCCGAAAGGCATGCCTGTTTGAGTGTCATGAAATCTCAATCCCTCTGGTTCTTCTGGACTGGTTGGATTGGACTTGGGTGATTTGCCAGCAATGGCTCGCCTCAAATGACTTAGTGGAACATCCCACATCAGTGTTAGACGTAATAAGTTTCGTCTCTCCTTGTGGTGATGACTGCTCAGAACCTGCCATCGCGCACTTTTGACATTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCACCCTTTGGTATTCCGAAGGGTACGCCCGTTTGAGTGTCATTGTATTCTCACCCCTCCTGACTTTTGTCGGTGAGGTCGGTGGACCTGGACGTCTGCAGTAGTCTTCTACGGCTCGTCTCAAATGGAAGAGTGTGACCCCCCTTTGTACGGTGTCATCCGGTGTGATAAGCATCTTCACTGGAGTGTCTTGGATGGGCAGTGTCCGTCTGAGAGCATCGTTGGGGTGTGGTAATGCTGCCAACCGTCTCTCTGGAGATAACTCTTATGACAATTTGACCTCAGATCGGGTGGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTTTAAACTCTCAAACCACGTCGTTCGTTCGACGTGGCTTTGGAATGGTGGGGGTTTTTGCAGGCCCTATTAGAGGTGTCTGCTCCCCTGAAATGCATTAGTGGTGTCTGGAGTGGAAACCCCGCTAGATGTGATAAAATATCTATCGTCTATGCCTTCCGCATGAAACAGATTGTGCTGCTTCAAAAACAATTGTTGACAAACTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACCTTGCGCCTCTTGGTATTCCGAGAGGCATGCCTGTTTGAGTGTCATTTAATTCTCAAACCTGAATTTTCTTTTGTAGTTCAGGCTTGGATTTGGAGTGTGCCGGCCTTGACAAGGTCGGCTCCTCTGAAATTTATTAGTGAAGAGGCTCTCTTATCCCCCCCGAAAGCTATTTTTGGCATTGATAACTTGTCTGTGCCTTTCCTGCTTGGTGGGATTTTTTTGTAGCCGCTCGCTCACAAATACACAATAGAAGTTATATTCTATTCTTTTTGACCAAATTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 10
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTTAGTGAATCATCGAATTTTTGAACGCATATTGCACCTCTTGGTATTCCTTGAGGTATACTTGTTCGAGCGCCGTTTATATTCTAAGGCATTGCCTTGTGTTGATGTTACCTGTCAAAGGTTCATCGAAATAAATGGGCACTTATTCTTTGTCCTAAGCGCAGCAAAATGTGTAAAGGTGGAGCATAAGTAGCCAGTGCATTTTCAAGCATGGCCTCGGATCAAGTAGGGTTACCCGCTGAACTTAA
## S1 0
## S2 10
## S3 0
## S4 0
## S5 1
## S6 0
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCGTTAGGCCGAGGGCACGCCTGCCTGGGTGTCACGCATCGTTGCCCCACCCCAAACCACCCCTCCCCTCTCGAAAGAGAGAGACGAGGGGACTTGGCCGTGGGCGGATATTGGCCTCCCGTGTGCCGAACGGCCCGCGGTTGGCCTAAATACGAGTTGTCGTCGGCGATGGACGTCGTGGCGTTCGGTGGTCAAATAAACCTCGAGCTCCCGTCGCGCGTACGTCGTCGGCCTAATAAGGCTCACCGACCCTGAAGCGTTGTGAACAGCGCACGCATCGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAA
## S1 0
## S2 0
## S3 0
## S4 1
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTAATATATATCAACCTCTCTTGTTGAGTGGTTTGGATGTGGGGGGTGTTTGCTGGCCTGTTCAGCTCCTCTGAAATACATTAGCAGAACAAACCTTGTTCATTGGTGTGATAACTATCTACGCTATTGAATGTGAGGTGCAGTTCAGCTTTCTAACAGTCCTTGGACAATTTATCATTTATGTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 1
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTGGGCCCCGCCGGTTCCGGCGGGCCCTAAAGTCAGTGGCGGTGCCATCCGGCTCCGAGCGTAGTAATTCTTCTCGCTCTGGAGACCCGGCTGTGTGCTTGCCAGCAACCCCCAATTTTTTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTCGGTATTCCGAGGAGCATGCCTGTTCGAGTGTCATGAATATCTTCAAATCCTCATTCCGTCCTCACGGGCGGCTGGGGGCTTTGGATTTGGACGTCTGCCGGGTTTGTCCTCGGCTCGTCCCAAATGCATTAGCTGGATCCCTACCCTTTCCCGGTCCTCGGCGTGATAGTTTCGTCTACGTCGTATTTGCCGGTCAGAAGGGTGGGCCGCTTCTAAACGGTCTTCATCGAAGACGATTCATCCAATTTGACCTCGGATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGTATTCCGGGGGGTATGCCTGTTCGAGCGTCATTGCAACCCTCAAGCACTGCTTGGTATTGGATGCTACCCACTGGTGGTGTGTCTTAAAAGTATTGGCGGCAGCATCTGGCTTCTAGCATAGTAATTTTCTTGCCTTGGAGTCCCGGATGTCTGGCTGCCAGAACCCCTAAATCATTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACCTTGCGCCTCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTAATATCCTCAAAAGAGACCTTTCTTGAGGAGGCCTCTTTTGGAAGTTGGGGTGTGCTGGCCCTTTGTTCTAGGGTTTGGCTCCTCTGAAATGCATTAGCGGTGTGACATCCACCTGCACATGCTTCCTTGGCATTGATAAGTCCTTCATGCCCAAGTCGCGGTGCAGCTGTGGCAAGGGGTTCACTCGCTCAAAGCTTTGCATGGGTTTTCCCCATCATTATGACCCAATGTTAACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGTATTCCAGGGGGTATGCCTGTTCGAGCGTCATTACAACCCTCAAGCACTGCTTGGTATTGGATGTCAACCATTGGTGGTGCATCTCAAAAGTATTGGCAGTAGCATTTAGCTTCTAGTGTAGTAAATTTCTCGCTTTGGAGTCAAGTGTCTAATTGCTAGATAGAACCCCTAATTTATCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 17
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAACCATCAAGCCTGGCTTGTCGTTGGACCTCTTTACCGCTGAAATATGTGGTAGGTCCGAAAGGTAATGACGGTGTCGTGTTTGACCCTAGATGCAACGAGCTTTTAATAGCACGCATTGAAGTGGTCGGCCGACCCGGTCTTAACCATCATTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 1
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAACCATCAAGCCTGGCTTGTCGTTGGATCTCTTTGCCACTGAAATATGTGGCAGGTCCGAAAGATAATGACGGCGTCGTGTTTGACCCTAGATGCAACGAGCTTTTTATAGCACGCATTGATGTGGTCGGGCGACCCAGTCTTTAACCATTATTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 1
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGTCCGGCGTTGGGGATCGGCCCTTTACCGGCCGGCCCCGAAATCCAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACACTCGCACCGGGAGCGCGGCGCGTCCACGTCCGTAAAACACCCCAACTTCTGAAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 3
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTAAATTCTCAACCCCACTAGCTTTTGTGAGCTATGCTGGGGCTTGGATGTTGGGAGTTTGCGGGCCTTTACTAGGTCGGCTCTCCTGAAATACATTAGCGGAACCGTTTGCAGTCCCGTCACTAGTGTGATAATTATCTACACTGTGATGACTGCTCTCTGACTAGTTCAGCTGCTAATCGTCCACTGTGGACAATCATTAATGAACTTCTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 2
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGATTTTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCTTTGGTATTCCAAAGAGCATGCCTGTTTGAGTGTCGCTAAATTCTCAAAACTCGACGGGGTTTTCAATTGACCCGGGTCGAGCTTTGGATTTGGAGTGTGCCGGGATTCTCTCTAGAGAGAATCGGCTCCTCCGGAAATCATTAGCGCGAGTTGGAGTTGAATGGGCTTTGGTTTGATAGCTATCTATGCCGTTGCCGTGAATGCTCCCGCGTCGCTTACAGTCGTCCCTGCGGGACAATTTGACCATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACTCTCTGGTATTCCGGAGAGTATGCTTGTTTGAGTATCTGTAAACATCTCAAATCTCTACTTTATTGTAAAGAATTGGACTTGAGCAATCCCAACTCCTTCGTCAGAAAGAGAAGGGTTGCTTGAAATGCAGGTGCAGCTGGACATTCTTCTGAGCTAAAAGCATATTTATTTAGTCCCGTCAAACGGATTATTATTCTTGCTTCAGCTTATATAAAGGTTGAATGACTACCAAGGCTGATTGCAGGTAAACTCGTTCCGTAAAAAAGAACTTGTAAACTCGATCTCAAATCAAGTAAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGCATTCCGAGGGGCACACCCGTTTGAGTGTCGTGAAATCATCAAAAACCTTTTTCTGTTCATCACAAAAGGATTTTTGGACTTGGAGGTTTAATGCTTGCCTTTCACTTTTGAAAGTGAGCTCCTCTTAAATGAATTAGTGGGGTCCGCTTTGCTGGTCCTTGACGTGATAAGATGTTTCTACGTTTTGGATTTGGCACTGTCTCTTTGGATTCCTGCTCCTAATCGTCCAACGGACAAACGATGGTGCTTCGGTCGCCGTCGCCTACATTGGCGGGGGAGGGCTGGACCCACGAAAGAAAACCTTGACCTCAAATCGGGTGAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGTGTCATTAAATTCTCAACCTTGCAAGCTTTTTTGCTAGCGAGGCTTGGATGTGAGGGCTTTTGCTGGCTTCCTTCATTGGATGGTCTGCTCCCTTTAAATTCATTAGTGGGATCCTTTGTGGATGGTCACTTGGTGTGATAATTATCTACGCCGCCTGACTCTGAAACAAGACTTGTGGGGACCTGCTCATAACCGTCTCTTCAAGGGACAATCTTTATTGACAATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGTGTCATTAAATTATCAACCTTGCTCGCTTTTATTAGCTTGAGTTAGGCTTGGATGTGAGGGTTTTGCTGGCTTCCTTCAGTGGATGGTCTGCTCCCTTTGAATGCATTAGCGGGATCTCTTGTGGACCGTCACTTGGTGTGATAATTATCTATGCCAGTTGACTTTGAAGCAAAACTTATGGGAACCCGCTCATAACCGTCTTCGGACAACTTTTGACATTTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGCATTCCGAAGGGCATGCCCGTTCGAGCGTCATTACACCACTCAAGCCTCGCTTGGTATTGGGCGGCGGGAGTCCCCCGCGCCCCTAATTCATCGGCTGGACGGTTCGAATCTCGGCGTTGTGGTTATTCAAATCGCTGGCGAAGACGACCGGACGTGCCGCTAAAATTCATTACAGGTTGACCTCGGATCGGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTTAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGCATTCCGGAGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCCCAGCTTGGTATTGGGTCGCGCCGCCTGGCCGACCCCAAAGACAGTGGTGGCGCCCCCCGGGCCCTCAGCGTAGTAATTCTTCTCGCTGTAGGGACCGCGGTGGTGTCGACACCATAAACACCATACGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTAGTATTCTGGGGGGTATGCCTGTTCGAGCGTCATTGCAACCCTCAAGCACTGCTTGGTATTGGATGCCACCAACTGTGGTGCATCCTAAAAGTATTGGTGGCAGCAATTCAGCTTCTAGTGTAGTAATTTTCTCGCTCCGGAGTCTGAATGCTTAACTGCCAGAACCCCTAATTTATTAAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 2
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCACACCCGTTTGAGTGTCGTGAAAATCTCAACCTTCTCGGTTTCTTCTGGACACCGAAGGGAGGCTTGGACTTTGGAGGCCTTTGCTGGCGTCTCTCTCTTTTGAGAAAGGGTCAGCTCCTCTCAAATGAATTAGCGGGGTCCTCTTTGCCGACCCTCGACATGTGATAAGATGTTTCCATGTCTTGGTTTATGGCTCTGTTGCCTTTGGGACCCGCTTCTAATCGTCTCGAGACAATGTTCGAGCGTGTCTCCCTTCTCGGGAAACACTCTCGAACCCACGAACCCTTGACCTCAAATCGGGTGAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 5
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGTGTCATTAAATTATCAACCTTGCTCGCTTTTACCGGCTTGAGCTGAGGCTTGGACGTGGGGGTTTTTGCTGGCTTCCTTCAGTGGATGGTCTGCTCCCCTTAAATTCATTAGCGGGATCTCTTGTGGACCGTCACTTGGTGTGATAATTATCTATGCCGTTTGACTTTGAAGCAAATCTATGGGACCCTGCTTATAACCGTCTCTTACCGGGACAATTTAATAACATCTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCACCCTTTGGCATTCCGAAGGGTATGCTCGTTTGAGTGTCATTGTACTCTCACACTCCCGATCTTGGTTGGGAGCGGTGGACTTGGGTGCTGCTGCTTACCGTGGCTCACCTCAAATGGATTCGTGCATCTCTTGGTTGGACATAGTACGGCGTGATAAGTATCTTCGCCGGCGCCTCACTCGAGGGTGGCCGATCGGGAGCTCTGTGCTTCTAACCGTCTCCGGACAACAACTGACAACTTGACCTCAAATCGAGTAGGACCACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGTGCCCCTCAGTACTCTGGGGGGCATGCCTGTCCGAGCGTCATTGCAACCCTCAAGCGACGCCGCTTGGTGTTGGGTGCCACCTTGGTGCACCCTAAAAGCAGTGGTGGGACCGCCCGGCTACCAGCGTAGTAGCATCTCAGCTTGTAGCGCCTGGGTGTGGGCCCGCCGTCGCCCTCTGTCAGAGAGTTGACCTCGGATCAGGTAGGGTTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTTTTTGAACGCAAGTTGCGCCCAAAGCCATTAGGCCGAGGGCACGTCTGCCTGGGCGTCACGCATTGCGTCGCCCCCACACCCTGTGTCCCCAAAAAAGGGGCATTGGGCGAGGGGCGGATATTGGCCTCCCGTGCTTTCGAGTGCGGTTGGCCTAAAAGCGAGTCCCCGGCAACGGACGTCACGACAAGTGGTGGTTGAAAAGCCTTCTTATCCTGTCGTGCGGTCCTCCGCTGCCATCGGGCACTCCCTTGACCCTGATGCGCCGTTCCTGACGGCACTTCGACCGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTAAACTCTCAAGCCCCGCTTGGTATTGGGTGCCGGCCCGTCAGGGCCCGCCCGCAACCCGTTGGGCGGGACCGTCGGGCTCTAAGCGTAGCAAGAATCTTCCCTCGCTTCGGAGCTCCGGCGGCCCCTCGCCCGGGAACACCATTCTCAAAGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTAACACACTCAAGCTCTGCTTGGTATTGGGAATCACTGGTCACCCAGTGTACCCTAAAATCAGTGGCAGTGCCATTCGGCTTCAAGCGTAGTAAATCTCTCGCTCTGGATGTCTGGGTGGTGCCCGCCAACAACCCCATCTTTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTGTGGTATTCCGCAGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCCCTGCTTGGTGTTGGGCCGGGCCCGTCGCGGCCGGCCCTAAAGACAGTGGCGGCGCCGCTCGGCTCTCAGCGTAGTACACCCCTCGCTGCGGTCCCGGGCGGCTGCCCGCCAGAACCCCGACTCTGTGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 10
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAGTTTTTGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTCTGGTGTATTCCGGAGGGCATGCCTGTCCGAGCGTCATTGTCAACCCTCAAGCCCTGCTTGCTGTTGGACCCCTCGACGAAAGTTGGCGGCCCCAAAGATAATGACGGTGTTGTGGCAACCCCCAGATGCAATGAGCTTTAACACGCACGCGTCACGGCGGTACCATGACCCCAGTCTTTAACAATTATTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCTATTGGTATTCCGATAGGTATGCTTGTTCGAACGTCATTAAACTCTCAAGCTGAGCTTGGTTTTGATCTTTGTCTTTCGACATTGATCGCAACTCAATGGTTTGTTCAACCTGATTCAAATGTAGCAATGTTATCATTTAGTATCAGGTTGTCCAACGCCAGGTAATCACCACTTCTAAAGTTTGACTTCGGATCAAGTAAGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTGCAACCCTCAAGCATTGCTTGGTATTGGGCTCCGCTGCTCACCCAGCGGGCCTTAAAATCAGTGGCGGTGCCGTCGAGGCCCTGAGCGTAGTAAATATCCTCGCTATAGGGACTCGGTGGACGCTGGCCATTAACCCCCAACTTTCTAAGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 16
## S2 0
## S3 0
## S4 1
## S5 0
## S6 0
## GCGCGATAGGTATTGTGAATTGCAGAATTGTGAATCATCGAATTTTTGAACGCACATTGCACCCATTGGTATTCCGATGGGTATACTTGTTTGAGCGTCATTTCATTCTCCTTTTGGGTTTTGGCATGAATATTTCTTGCTGAATTATAATGGTGTGGCTACCAGACTACAACGTGATAGATATTTCGTTGGATGTGACTGGGATTGCTCACCTTAAAAACATTGTATAGACCTCAAATCAAGCAGGATTACCCGCTGAACTTAA
## S1 11
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATCCATCCCGAGGAGCATGCCTGTTTGAGTGTCGTGAAATTCTCAATCTCGTTAAATTTTTTTGACGAGTTTGGATTCTGGAGGCTTTGCTGGCGAAAGTTGGCTCCTCTGAAATGCATTAGTGAAACCTTTCCAAACGGAAAACGGGCTTTGGTTTGATAATTGCTTTTGTCTATGCCATTGCCGTCGCCGTGATGATTTCGCTTACAACTGTCCTTTGGACAAATCTTGACCTCATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATCCAGTGAATCATCGAATCTTTGAACGCATATTGCGCCCTTCGGCATTCCGAAGGGCATACCTGTTCGAGCGTCATTACAATCCTCAGGTGATATCATCTGATGTTGGGTGTTGCATGGAGCATGCCCTAAAGCCAACGACGGCGGCGTGCCGACCTCAAGCACACAGCAGTGCGATGCTGGGAAGAGCGCCGTCGGGACCCCTTCCAAGACGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATAAGTAATGTGAATTGCAGAATTGTGAATCATCGAATCTTTGAACGCACATTGCGCCTTTTGGTAATTCCAAAGGGCATACCTGTTTGAGCGTCATTTCACTCTTAAATCGTTTGATTTAGTATTGAACTTATATTTTTCTGTTGCAAAATAGAAAATAGTTTGAAATTGATTTGGCAAGAAGCAATATCCAGTTTGAAGCTTTCTTTTTACAACGTAATAGGTTTTACCAACTCGTTGGCTAGTAAGGAGCTTTGTTATATTTGGATATATCGCTTTGGCGTTATAATTTTAAAATTAAAAGTTTGACCTCAAATCAGGTAAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTGGGCCTCACCCGTCAGGGTGGGCCTTAAAATCAGTGGCGGTGCCATTCGGCTTCAAGCGTAGTAATTTTCTCGCTCTGGAGTACCGTTTGCGTGCCTGCCAGCAAACCCATATTTTTTAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGCATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTACCCTCAAGCGCGGCTTGTGTGTTGGGCGCTGTCCCCCCGGGGACAGGCCTGAAAGGCAGTGGCGGCGTCGCGTCCGGTCCTCGAGCGTATGGGGCTTTGTCACTCGCTCTGTGGGGTCCGGCCGGGGCCTGTCTACCCCAATCTTTTCTCAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCTTGAAGTCATCAACCCCTCCCGTTTCGCACGCGGAGAAGGTTGGACGTGGGGGCTTGCGGTCGTGAGGCCGCTCCCCTCAAATGCTTCGGTCGGGCCTGTGGGTCGGACTCGGTCGGGCGTTGTACCATCCTCGCTCTCCTCCGAGAACTTCCCCGTCGATAGAGCCCTTCCACGGCCTCCTATGGAGCGGTGGCCAAACAAACACTTTGAAATCTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGTATTCCAGGGGGTATGCCTGTTCGAGCGTCATTGCAACCCCTCAAGCACTGCTTGGTATTGGATGTCACTATTTTTGTGCATCTCAAAAGCATTGGCAGCAGCACCTAGTCTCTAGTGTAGTAAATTTCTCGCTATGGGTCTAGGTGTCTGGCTGCCAGAACCCCTAGTTTTTCTACAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATCTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGTGTCATGAAAACATCATCCCATCGGGTTTCCGATGACGATGGATTTGGGTGTTGCCGCCTCGGCTCGCCTTAAAAGTGTTAGTGGTTCTAAGATTGAATCTGGCGTAATAAGTTTCGCTGGCCAAGATCAAAGGCTGCTCATAACCCGTCTTCTGACATTTTTTGCTCTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTCGAGCGTCATTATCACCACTCAAGCTCTGCTTGGTATTAGGCTGCGTCGCCCCCCCGGGCGGCAGGCCCCAAATCTAATGGCCGCCCCTATCGGGTCCCGAGCGTAGTAGCTTTTTTGCTCGCTCACCTGGGACCGCGTAGGCTGGCGCGTCGCCTTTACATATCATCTGGTTGACCTCGGATCAGGTAGGAGTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTCGACACAGGAGGGTATGCCTGTTTGAGTGTCATTTATTCCTCAACATAACTAAGTTATAATAACTTAACTTGTGCTTGGATGTTGAAGGTTTGCTGATAAAATCATGTCAGCTCCTTTCAAATATATGAGCAGGACAGCATGTAACCAGCTCCAATTTGATAAGTAGTTATCTGTGTTGTTGCTGGGTACAGTTAAATGTCTGCTACCAATTGTCCTACTATTGGGACTTGCCTTCTAGGCAAATCTATTGAAATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCACCCTTTGGCATTCCGAAGGGTATGCTCGTTTGAGTGTCATTGTACTCTCACACTTCTGATTCTTTGGTGGATTGGGAGCGGTGGACTTGGGTGCTGCTGCTTTACCGTAGCTCACCCCAAATGCATTAGTGCAACTCTTGGTTGGACATAGTACGGCGTGATAAGTGTCTTCGCCGGCACCTCGCAAGAGGGTGGCCGATCGGGAGCTCTGTGCTTCAAACCGTCTTCGGACAACAACTGACAACTTGACCTCAAATCGAGTAGGACCACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAATCATCGAGTTTTTGAACGCACATTGCGCCCGCCAGCATCCTGGCGGGCATGCCTGTTCGAGCGTCATTTCCACCCTCAGGCTCTGCCTGGTGTTGGGGGCCTGCACCCGCAGCCCCTAAAAGACAGCGGCGGTCCCGGCCCCGGCCCCGAGCGTAGTAATACACGTCGCTCCGGGCGCCGGGCGGGCCCCAGCCGTAAGCCTTTTTTTCATCAATAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTCGACACAGGAGGGTATGCCTGTTTGAGTGTCATTTATTCCTCAACGCAACTAAGTTGTAATGACTTAACTTGTGCTTGGATGTTGAAGGCATGCTGGTACAAACCATGTCAGCTCCTTTCAAATATATGAGCAGGACAGCATGTAACCAGCTCCAATTTGATAAGTAGTTATCTGTGTTGTTGCTGGTACAGTTGAATGTCTGCTACCAATTGTCCCCTACTATTGGGACTTGCCTTCTGGGCAAATCTATTGAAACTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCTTCTGGTATTCCGGGAGGTATGCCTGTTCGAGCGTCATTGCGACTAACAAGCCTAAGCTTGGTATTGGATGTCGCCAGCTGGTGCATCTTAAAATTAGTGGCGGTGCCATTCAGCTTCAAGCGTAGTAAATTTCTCGCTTCTGGAGTTTGGGTGTTGTGCCTGCCAGAACCCCTAAATTATCAAGGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGCTATTCCGAGGGGCATGCCTGTTTGAGTATCATGAACACCTCAACTCTCATGGTTCGCCATGATGAGCTTGGACTCTGGGGGTTTTGCTGGCCTGTGGTCGGCTCCCCTCAAATGAATCAGCCTCCCAGTGTTTGGTGGCATCACGGGTGTGATAAATATCTACGCTCGCGGTTGTCTGCCAGGTAACCTTTGGTGACAAAGGTTCGCTGGAGCTCACAGATGTCTCTCCTCGGCGAGGACAGCTTTTTTGAACGTTCGATCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATGACCACTCAAGCCTAGCTTGGTATTGGGGTTCGCGGTTCCGCGGCCTCTAAAATCAGTGGCGGTGCCGTCTGGCTTCAAGCGTAGTAATTTTCTCGCTACGGAGTCCGGGCGGTTGCTTGCCAGAACTCCCCAACTTCTCACGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 2
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATCGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGCACTCAGGGGGTATGCCTGTCCGAGTGTCGTGACGAACCTCGAGCTCCCTGGAGCTTGGCGTTGGGTGCGGCCCCCGTAACTGGGGGCCCTCCCCAAAACCAGTGGCGGCCGCGGCCCGGTCCCGAGCGCAGTAGACTTCTCTCGCTCCGGGGCCCGGGCTGCGTGCCTGCCAGCAAGACATCAGTCATACCCCCAAGGTACGACCTCGGATCAGGTAGGAGCACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGCATTCCGGAGGGTATGCCTGTTCGAGCGTCATTACAGCCATCAAGCACTGCTTGGAGTTGGGCGTCGCCGGCTCACGGCGCGTCCCAAAATCAGTGGCGGTACCATCTGGCTCCGAGCGTAGTAATCTCTCTCGCTTTGGAGCCCAGATGGGGCCCGCCAGAAGCCAACTATATCATAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCCCCCGGGCTTGGTGTTGGAGATCGGCGTGCCCCCCGGGGCGCGCCGGCTCCCAAATATAGTGGCGGTCTCGCTGTAGCTTCCTCTGCGTAGTAGCACACCTCGCACTGGAAAACAGCGTGGCCACGCCGTTAAACCCCCCACTTCTGAAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTAGGCCTCACCTGCTAAGGCGGGCCGTAAAATCAGTGGCGGTGCCATCTGGCTTCAAGCGTAGTAAATTTTCTCGCTCAGGAGTACCGGGTGTTTACCTGCCAGCAACCCCAAATTTTTACAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGCAGTGCGAATTGCAAAACGTGAGTCATCAGATTTTTGAACGCAAATGGCGACAGCGCAAGCTGTCATGTTTGTTTCAGTGAACCAGGTTAATAACATCACATAAGAAGATGCGATTGAAGTAATTCTTTCGCTAAGTACGTAGTAGTTTGGCTTAGACCACTATTAACCGAAGTAGTCACTAAAAAGTGATAATCGTGTTTATAGAGGCTAAGCCTTAAAAGAACTACTAAAACAACTTTTTATCCACTAACCTGAAACAAGCAAGATAACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGTATTCCGGAGGGTATGCCTGTTTGAGTGTCATAACCATTCTCAGTGAGAAAAACATTTTAACCAAATGTGTTTCTTGCTGGATTTTGAAGGCTTTGCTGGTTGCCTCTTTGCAGGACCTATCAGCTCCTTTTAAATTCATTAGCAGGTATCAATGCTTGAAATGGCTTCAGTATAAAGTTTACTTTTGAAGTTATGAAAAGCTTTCATTTGACCCGCTTTCAAATAGTCCCTTTGGGACAATAAAGAACTTTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 3
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATCTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGTGTCATGAAAACATCATCCCATCGGGTTTCCGATGACGATGGACTTGGGTGCTGCCGTCCGGCTCGCCTCAAAAGTGTTAGCGGTTCCAAGTCGAATCTGGCGTAATAAGTTTCGCTGGCCAAGACGTAAGACTGCTCATAACCCGTCTTCTGACATTTTTTGCTCTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 3
## S3 0
## S4 0
## S5 0
## S6 0
## GTGCGATAAGCAATGCGAATTGCAGAACCGTGAGTCATCAGATTTTTGAACGCAACTGGCGCCGACTGGCTCTCCAGTCGGCATACTTGTTTCAGTGTCTCTTTATCTCCTAACCCATATCATAATGCGAGAGATATCCTTCTCTTGCTAAACATGAAAGCACTCTGCGCTCAGCGAGTGAAGTCGACTTGTTCGATTTCACACTCAATGCAGCAGTCACATTGCTCACAATGTGAACTCATTGAGAGCTGAGGCAGTGCAGCGTAAGCTGCCAGTGCTTAGTCACACAAACTTACTTGCATCTGAAATCAAGCAGGATCACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 4
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGGTCGGCGTTGGGGATCGGGAACCCCTAAGACGGGATCCCGGCCCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCATGCGCAGTAGTTTGCACAACTCGCACCGGGAGCGCGGCGCGTCCACGTCCGTAAAACACCCAACTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 2
## S5 1
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATGAAACTCTCACCCTCTAGCTTTCTTAATCGTGGCTAGCGGCGTGGACGTGAGCGCTGCTGCTTTGTTGCGGCTCGCTCGAAATGCATTAGCAGACCCTTTTCGTAATCGGTTCCACTCAACGTGATAAGTATTTCGTTGAGGACAGTTGCAGCAATGCGGCTGGCCGGGATAAGAAAGGCATAGTTGTCAGCTTCTAATCGCCCTTGGGCAATTTTTTATGATCTGGCCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGACATTCCGAGGGGCATGCCTGTTTGAGTATCATGAACACCTCAACTCTCATGGCTTGCCCGTGATGAGCTTGGACTTTGGGGGTTTTGCTGGCCTACGGTTGGCTCCTCTCAAATGAATCAGCTTGCCAGTGTTTTGGTGGCATCATGGGTGTGATAACTATCTACGCTTGTGATTGTCTGCTGAGTGACCTTCAGCGATGGAGGTTTGCTGGAGCTTACAGATGTCTCTCCTCAGCGAAGACAGCTTTTCGAACGTTCGATCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCACCAGTACTCTGGTGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAGCCCCCCCGCAGCGGGCTCGGTGATGGGACGCTACAGCGCTTCGCTGCGCTGTAGGCCCCGAAATACAGTGGCGGCCTCGCCGAGACCCCGAGCGCAGTAAACCGTGTCTTAGCACAGCGCCTCGCTCAGGGCGTCCGGCGGGTGCCCCGGCCGTTAAACCCCCAACCTACATAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCACCACTCAAGCCTCGCTTGGTATTGGGCAACGCGGTCCGCCGCGTGCCTCAAATCGACCGGCTGGGTCTTCTGTCCCCTAAGCGTTGTGGAAACTATTCGCTAAAGGGTGTTCGGGAGGCTACGCCGTAAAACAACCCCATTTCTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGTGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCTCTGGTATTCCGGAGAGCATGTCTGTTTGAGTGTCATGAACTCTTCAACCTACCGATTTCTAGTAAATCGGATTAGTGTTTGGATTTTGAACGCTTGCTGGCCTTTATTGGCGAAGCTCGTTCGTAAATGATTAGCATCTCATATTCGAAAATCGGATTGACTCAGTGTAATAGACTATTCGCTGAGGACGCTCTTTCGGGAGTGGCCGAGTTTTGTAAAGTAGAAGCTTCTAATTCTATTAGTCAACTTTAGATTAGACCTCAGATCAGGCAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGGTAGCACTCTACCGGGCATGCCTGTTCGAGCGTCGAACTACCCTCTATTACGGTGTTGGGGACTACTTGTAGCCCTGAAATATAGTGGCGGCCGTTCTAAGCCTTTGCGAAGTCCAATACGCAACAGGGGATGAAATCGGTTCTGCCTTGTTTACCACTTTTGAAGACGACCTCGAATCAGGTAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTCGAGCGTCATAATGACCAAATCACCCTTGTGGTGGACTTGGAGGTGGCCAGTACTGGCCTCTCTTAAAACCAGTGGCGGTGCTCTTAAGCTCTACGCGTAGTAATTTTTCTCGCTATAGGGTCTTGATTGTGCTTGCCAACAACCCCAATTTTTTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATAACCAATCCAGCTTGCTGGGTCTTGGGCACCGCCGGCTGGCGGGCCTCAAAAGCAGTGGCGGTACGGCCGGGCTCTAAGCGTAGTAATACTCCTCGCTACTGACCCCCGGGCGGCACCTGCCAGAACCCCCCATTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGACACCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTACACTATTCTTCGGAGTATTGGCACTGGCTCACGCCTTGCCTAAAACACTTGGTGGTAACTATCAATCACGCATAGTAATCTCTGCGAAGGCTGGTAGGTAAACACCTTAAAACTCTTTTGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCGTGGTATTCCGCGGGGCATGCCTGTTCGAGCGTCATTATGACCACTCACGCCTGGCGTGGTGTTGGGGCACGCGGTTCCGCGGCCCTCAAATCCAGTGGCGGCGCCGGTTGGCTCTAAGCGTAGTAAAACTTCTCGCTTCAGGGTCCTCTCGGTGGCTCGCCAGAACCCCCCAACTTCTTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGTGATAAGTAATGTGAATTGCAGAATCCAGTGAATCATCGAATCTTTGAACGCATCTTGCGCTCCTTGGCATTCCAAGGAGCATGCCTGTTTGAGTGTCGTTAAACTATCAAAACACTTTTCTTGGGTGTTTTGGACTTTGGGAGTATCTTGCTGGTCTGTTTGAGCCAGCTCTCCTTAAAAGCATTAGCTTTGGAGACCATCAGTGTGATAATTTGTTTACACTGACTTGAGTGTCTCGGCTTCTCATTTTGATACCCCTTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCACTGCTTGGTGTTGGGCCTCGCCGCTAACCCGGCGGGCTCTAAAAACAGTGGCGGTGCCGTCGGGCTCTGAGCGTAGTAATTCTTCTCGCTATAGAGTCCCGGTGGATGCTGGCCAACAACCCCCAATTTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 1
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGTCCGGCGTTGGGGATCGGCCCTTAACCGGCCGGCCCCGAAATACAGTGGCGGCCTCGCCGCAGCCTCTCATGCGCAGTAGTTTGCACACTCGCACCGGGAGCGCGGCGCGGCCACAGCCGTAAAACAAACCAAACTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 1
## S2 0
## S3 0
## S4 0
## S5 2
## S6 0
## AAGTGCGAAAAGTAATGCGATTTGCATGCTCTGTGAGTCATCGAATCTTTGAACGCATATGGCACCTGCCAGCCCTGCTGGAAGGTATGCCTGTTTGAGAATCATATCCACATGATGCTCCACGGAACATCGACCTGGGCCCTCACCACTGATCGCCCCGAATTGCTCGCGGTTGTCCTGCTAGTCCATTTGGCAAACTTGACAATCACAACCCCCCATTTCATGATCTCAAATCAGGCAAGGCTACCCGCTGAACTTAAGCAT
## S1 0
## S2 0
## S3 0
## S4 0
## S5 1
## S6 0
## ATGCGACAAGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCTGGAGGGCATGCCTGTCCGAGCGTCATTGCTATACTCAAGCCCAGCTTGTGTTGGACTCACTGTCTTGTTGGACAGTAGCCCCAAAGGCAGTGGCAGGGTCGCAGTCCGGTCTTTTCAATGCGTATGGGGCATTGTCACCCGCTTGAAAGCTGGCCGGCCCTTTTCAGCCTATACAAATCTTTTTTTTTCTGATGGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCCGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCGCCAGCATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAGGCCCTGCCTGGCGTTGGAGGACTGCACTGCAGCCTCCCAAAGCGAGCGGCGGCGGCGCCCCACAACCGAACGCAGTAGTTATACCTCGTTCTGGGCGTGGGACGTTGTCCTGCCGGAAAACACCCCCTTTTTAATTGTTGACCTCGAATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCACACCCGTTTGAGTGTCGTGAAAACCTCAACCTCTTTGGTTTCTTCTGGGGACCAAAGCAGGCTTGGACTTTGGAGGCCTTTTGCTGGCACCTCTCTCTTTTGAAGGCCAGCTCCTCTTAAATGAATTAGCAGGGTCCTCTTTGCCGATCCTCGACGTGTGATAAGATGTTTCCATGTCTTGGTTTCTGGCTCTGTCACCTTTGGGACCCGCTTCTAACCGTCTTGACCTTGCGTCGAGACAATGTTCGAGCATGTCTCCCCATTCTCGGGAAGCTCCCTCGACCCCACGAACCCCTTGACCTCAAATCGGGTGAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCCTCAAGCCCAGCTTGGTATTGGGCTCGCCCCTTGTGGGCCTGCCTCAAAATCAGTGGCGGCTCCGTCCGGTCTCAAGCGCAGTAATACTCGTCGCTTGTTTAGGCCTGGTCGGCGGCCTGCTAACAACCCCCACTTTTACAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGTATTCCAGGGGGTATGCCTGTTCGAGCGTCATTACAACCCTCAAGCACTGCTTGGTATTGGATAACACCTGTTGTGGTGTGTCTCAAAAATATGGGCAGTAGCACTCAGCTTCTAGTGTAGTAATTTTCTCACTTTGGAGTTTTGGGTGTCTAGTTGTTAGAACCCCTAATTTTTAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTGTGGTATTCCGCAGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTCTGCTTGGTGTTGGGCCACGCCCCCGCGGCCGGCCCCAAAGCCAGTGGCGGTACCGCCCGGCTCTAAGCGTAGTACACCTCTCGCTCTGGAGCTCGGCGGTTCCCTGCCAGAACCTACACTCTTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGCATTCCGAAGGGCATACCTGTTCGAGCGTCATTATCAACCATCAAGCCTAGCTTGTCGTTGGACTTTCTTTTACGGTTGAAATATACCGTAGGTCCGAAAGATAATGACGGTGTCGTGTTTGACCCTTAAATGCAACGAGCTTTTATTAGCACGCATTGAAGTGGTCGATCGACCCGGTCTTAACCCCATTAATTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCTCTCTGGTATTCCGGAGAGCATGCTTGTTTGAGTATCAGTAAAACACCCTCAGCCTTTTCTTTATTGAAAAAGCTGGACTTGAGCGATCCCAACGGTTTTTTTTTAAAAGGCCGGCGGGTTGCTTGAAATGCAGGTGCGGCTGGACACATCTTCTGAGCTATAAGCATTACCTATTTAGTCCCGTCAAACGGATTATTTCTATTGCTACAGCTAATATAAAGGTCGATTGTCCTTGTGCCCGACTGATGCAGGAAAACATGGAGAAATCCTTGTTCAACTCGATCTCAAATCAAGTAAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCTTTTGGTATTCCGAAAGGCATGCCTGTTTGAGTGTCATGTAGACTCAACTCTCGAGAGGTTTCTTGCGAGTTGGATCTGGGTGTTGCCATCTGGCTCACCTTAAAAGCGTTAGTGGTTTGAGCACTTGATCGGACGTGATAAGTTTCGTCCGGCCGTGTGTCGTCGACCGCTCCTAAATTGCCTTGCGGCGATCTTTGACTCTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGTATTCCGGGGGGTATGCCTGTTCGAGCGTCATTGCAACCCTCAAGCATTGCTTGGTATTGGATGCCACCACTTGTGGTGCATCTCAAAAGTATTGGTGGTGGCATTCAGCTTCTAGCGTAGTAAATCTTCTCGCCTTGGAGTTTGAGTGTTCAACTGCTAGAACCCCTAAATTTATCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCTTTGGTATTCCAAAGAGTATGCCTGTTTGAGTGTCATGAAATTCTCAAGTCTGTAGATTTTTTAAAAATCTGCGTGACCTGGACTTGGAGGTTGTGTCGGCTTTGTCAGTCGACTCCTCTGAAATGCATTAGCGCGAACGTTTACGAGTTCAGTGTGATAATTATCTGCGCTGTGATTGTAACTGTTTCGTGCTTTACCGTCTTTAGAGACAATTTTTGACATCTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCACCCTTTGGTATTCCGAAGGGTATGCCTGTTTGAGTGTCATTAAATTCTCAACTTCAAATTGAATTTTGAAGCTTGGACTTTGGAGGTTTGCTGGTGTCACTATCAGCTCCTCTTAAATTCATTAGCGGAACTGTAAGGACCGGCTTTGGTTTGATAGCTAACATTATCTATGCCGTTGCTGTGACCTTTGTGTTTGGCTTCTAATGGTCATTTTGTTGACTGTCTCTGCTTTGAGGCATACACTTTTAAGCTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 6
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAGCCCCTCAAGCAACTTGCTTGGTGTTGGGGATCCAGGCGATGGAGCCTGGGCCCCGAAATACAGTGGCGGGCCTGACAGGACCCCCGAGCGCAGTAGTCTTACAGCCTCGCTCAGGGCGGACTGCCGGTGCCCACGCCGTAAAACCCCCCAAATGCCCAAAAAGGTTGACCTCGGATCAGGTGGGAATACCCGCTGAACTTAA
## S1 1
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTTTAGTATTCTGAAGGGCATGCCTGTTCGAGCGTCATTATCACTAATCAAGCCTGGCTTGCTGTTGGGTCTCTTTGTCGTTGAAACCGACAGGCCCCAAAGATAATGACGGCGTTATTTGGACCCTAGATGCAACGAGCTTTCATTAGCACGCATTTAGCCGGACCTTTTAACCCGGTCTTTTTAACCAATTTTCTCAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 11
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCACGTGGTATTCCACGGGGCACGCCTGTTTGAGTGTCACGTAAACCCATCGCGTCGCGGATTGTCATGATCTGCGGGGCGGATTTGGACGCTGCCGTAGTTGGCTCGTCTCGAAATGCATTAGCCAGTCCGAGGACGCGCGCCACTGGTGTGATAATTCACTTTGCGCCGCTGGTGTGTCCGACGAAGGCTCATAATCGCCCGCAAGGGCAACATTTTTAATGCTCTGGCCTCAAATCAGGTGGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 2
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGCATTCTGAGGGGCACACCCGTTTGAGTGTCGTGAAATTCTCAAAACCCTTTCTTTTGGTCATTTTTGACCTTAGAAAGGATTTTGGACTTGGAGGCCTTTTGCTGGTTTCACCTTGAAGCGAGCTCCTCCCAAATGTATTAGTGGGGTCTGCATTGTCGGTCCTTGGCGTGATAAGTTGTTTCTACGTCTCGGATTTTTGGCGCTGTACGCCCGCTTCCAACCGTCTCATTGACAACGATGGTGTTCCGGTCGCCGCCGATTTTTTGGCGGGAGGCTTGACCCACGAAACAAACCTTGACCTCAAATCGGGTGAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATATTGCGCTCTCTGGTATTCCGGAGAGCATGCTTGTTTGAGTATCAGTAACCACCTCACATTCTTTTTTTCTTGTAAAAAAAGGATTGGACTTGAGCGATCCCAACGCGTCTGAATTCATTCGGGCGCGGCGGGTTGCTTGAAATGCAGGTGCAGCTGGATTTTTATTCTGAGCTAAAAGCATATTTATTTAGTCCCGTCAAAAGGATTATTACTTTTTGCTGCAGCTAATATAAAGGAACATTAATCCTCATGCTGACTGATGCAGGAATTTTCTTGTCGATGTTTCTTTTAACTTGTTAAGAGAAAGATCAGCAAGAAAACAAAACTCGATCTCAAATCAAGCAAGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTTACACCACTCAAGCCTGGCTTGGTATTGGGGTTCGCACACCAGCGGCCCTTAAACTCAGTGGCGGTGCCGTGTGGCTCTTAGCGTAGTAATTTCTCTCGCTATAGGGTCTCGTGGTTACTTGCCAGCAACCCCAACTTCTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTTCCGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCACCTCTCAAGCCCTGGCTTGTTGTTGGACTTTTGGTCCCGCCCTCGTGGCGCGACCGGTTCCAAAGACAATGACGGCGTCCGTGGGACCCTCGGTACAATGAGCTTTCACCAGCACGTATCAGAGTTGAAAGGTTCTCGGGCCCGGTCTCCTTTTATTTTAACAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTTCCGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTTTGGCATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCCTCCCTCAAACCCCCGGGTTTGGTGTTGGGCCGCGTTGGACCGTTCGCGGCCAACTGGTCTCAAAGACAATGACGGCGTCCGTGGGACCCTCTTCGCACTGAGCTTTCCGGAGCACGCGTCGAGTTGCAAGGTCGTACGGGCCCGGTCACCTTCAGGGTACTTCGGTACCCTAAACTTTTTCAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATCAAGTTCTCAACCACACCAGGTTTTCTTCCTGCGACGTGGCTTGGATAATTGGGGGTCTCTATTTTGCAGGCTTATTTTAAAAGGTCGGCTCCCCTGAAATGCATTAGTGGTGTTCTGAGCAGAAGGGGGACTCTACAGACGTGGTAACCCGCATTTGTCTGTGTCTTCTCTGTCCATGCCGCTTGAATGATCATTTTCTTTTTGACAAATTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATATGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCGCCAGTACTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAGCCCAGCTCGGCGTTGGGGCACGCGGGTCGGGGCGCCCCCCTTCTGGGGGGGCGCCACCCCCCGCGGCCCTCAAATACAGCGGCGGCCGCCTAAGGGATACCCTCTGCGCAGTAAACTAACTCGCACCGGGAGCCCTACGGGAGGCCCGCCCCGAAAACCCCAACGCGCGCCCACCCCTCGGGGAGCCGCGTTCACAAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTTAGTGAATCATCGAATCTTTGAACGCATATTGCACCCCTAGGTAATCCTTGGGGTATACCTGTTCGAGCGCCGTTATGTCTTAGGCATGGCCTTGCGTTGATGTTACCCCGTTAGGGGTTCGTCGAAACATATTGGCGTCTTCTCTTGGCCCTAACCGCAGCGAATATGCGATGAGGGTAGGAGGGGGCAGCCAGTCTTCCGAAAACATGGCCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 1
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGCACTCTGGCGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAGGCCCTGCCTGGCGTTGGGGCTCTGCCGTACGGCGGCCCTCAAATACAGCGGCGGATGCCTCTTGCGAGACAATAACGTAGTAAATATCCTCGTTAGAAATGCTCGCGTCGTGTCCGGCCGTAAAACAGTGCCTCATCAGTCCCCTGGACTGCCCGGGCGCGCTTCTATGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 1
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCGTTAGGCCGAGGGCACGCCTGCCTGGGTGTCACGCATCGTTGCCCCCCCCCAAACCACCCCTCCCCTCTCGAAAGAGAGAGACGAGGGGACTTGGCCGTGGGCGGATATTGGCCTCCCGTGTGCCGAACGGCCCGCGGTTGGCCTAAATACGAGTTGTCGTCGGCGATGGACGTCGTGGCGTTCGGTGGTCAAATAAACCTCGAGCTCCCGTCACGCGTACGTCGTCGGCCTAATAAGGCTCACCGACCCTGAAGCGTTGTGAACAGCGCACGCATCGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAA
## S1 0
## S2 1
## S3 0
## S4 0
## S5 1
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCTTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACACCCCTCAAGCCTAGCTTGATATTGGGCTTGGCTAATTAAACAGGCCAGCCTTAAAATGAGTGGCAGTACTAACTCAGCCCTAAGCGCAGTAAATATTTTACGCTTTTGGGCGTGGTTTAGTTGCTTGCCAGAAACTACCAAATTCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 4
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCACCCCTCAAGCCCCTGGGCTTGGTGTTGGACGGTTTGGTGAAGCCTCCGGGCGTCACCCCTCCTAAAGACAATGACGACGGCCTGTTGGAACCCCCGGTACATTGAGCTTCTTAACTGAGCACGTATCGGATCAAGGGCACCCAGGCTCCGGTCTTCTCCCTTCATCGGGAAACCCTTTTCTCAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 1
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATATAGACCAACTCCCGCTTCGGCGGGGTCTTGGGCTTCGCCTCTGGGCGGGCCTTAAAACCAGTGGCGGCGCCTGCTTGGCTCTACGCGTAGTAATACTCCTCGCGATAGGGACCGGTGGACGTTGGCCAGCAACCCCCAATTTTTAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGCATTCCGGGGGGTATGCCTGTTCGAGCGTCATTACAACCCTCAAGCCTTGCTTGGTGTTGGGCGCCACCTGCTATAGGGTGCGCCTCAAAATCAGTGGCGGTGCTGCCTAGCTTCAAGCGTAGTAACTCTTCTCGCTCCGGAGCCCAGGCGGCATACCAGCCAACAATCTCAAACGATACATCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCGTGGTATTCCGCGGGGCATGCCTGTTCGAGCGTCATTTCAACCAATCAAGCTTCTGCTTGGTCTTGGGGCCTGCGGTTTCGCAGCCTCTAAACTCAGTGGCGGTGCTATTGAGCTCTGAGCGTAGTAATTTTTCTCGCTATAGGGTCTTCGTAGTTACTTGCCAGTAACCCCCAATTTTTATCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTGTAATCTCTCAACTTTGGCCTTGGCTGGAGTTTGGATGGTGGAGGTGTGCTGGCGGCCCCCGTGTCGGTCAGCTCCTCTGAAAGAAATCAGCAAAGGGAAACGCACCTGTTCCTGCATCTTGTGCTTTTGGCATTGATAATTGTCTATGCCTGTTGTGGCTCAGATGCCTTTTGGATCGGTGAGCGTCTTTTGCTTACAATGTTGTCCTTTGGGACATTTTTGACAAATCACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTGGGCCCCGGGCGTCAGCCCGAGCCCTAAAATCAGTGGCGGTGCCGTCTGGCTCTAAGCGTAGTAAATCTCTCGCTATAGGTGCCCGGTGGATGCTAGCCAGAACCCCAAATTTTTACAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACTCCTTGGTATTCCGAGGAGTATGCCTGTTTCAGTATCATGAGCACTCTCACACCTAACCTTTGGGTTTTGTTGTGGAATTGGGATGCGCCGATTTTAATAGTCGGCACTCCTAAAATGTAGTTCTTGGCTGTTTCCTACTACAGCAGTTTGGCCTAATAGTTTTGACTTTTGTCAAATCTTTGGCTACATTTGCTTCTGGAAATCAGTCTTGATAATACAGAAAACTCATTCAAACTTTGATCTGAAATCAGGTAGGGCTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGCATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCGCGCCCATCTGGGCTGCGGTGTTGGGGACCAGACTGCCCCCCGGGGCGGTGCTGCCCCCCAAATTTAGTGGCGGTCTCGCTGCAGCTTCCCTTGCGTAGTAGCACACCTCGCATTGGAACGCGGCTTGACCACGCCGTAAAACCCCCGACTCTATGAGTTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCCTTGCTTGGTCTTGGGCCCTGCCGGCAACGGCTGGCCTTAAAACTAGTGGCGGTGCCATCGAGCTCTACGCGTAGTAATTATATCTCGCTTCTGAGTCTTCGGTGGACGCTGGCCAGTAACCCCCAATTTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTAGGCCTCGCCTGTATGGGCGGGCCTTAAAATCAGTGGCGGTGCCATTTGGCTTCAAGCGTAGTAATTTTTCTCGCTCTGGAGGACCGATTGTGTGCCTGCCAGAAACCCCAATTTATCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCAGCTTGGTATTGGGCCTCGCCGGTTCCGGCGGGCCTCAAAGTCAGTGGCGGTGCCATCCGGCTTCAAGCGTAGTAACACTCTCGCTTCTGGAGACCCGGGTGCGTGCTCGCCAGCAACCCCAACTTTCTCAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCACTCCTTAGTATTCTGAGGAGTATGCCTGTTTGAGTGTCATTGAATATTCTCAATGCTTTTGGAAATTTGGGGTATGCTTTTGTTAGCTACTCTTAAATGTATATGAAAAAGCTTCAATTGGTATAAAATCGTTATTGAAGGCTTTTATTTGACACTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAAGCATAT
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTATGACCACTCAAGCCTGGCTTGGTGTTGGGGTCCGCGGTCCCGCGGCCCTTAAAATCAGTGGCGGCGCCATCTGGCTCTAAGCGTAGTAATACTCCTCGCTACAGAGTCCCGGTGGATGCTTGCCAGCAACCCTCAATCTCACGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTTAACTTCTCAAACCGCTTGATATATCAAGTGGCTTGGATTTGGGGGTCGTTTGCAGGCTTTTTCCAAGGTCGGCTCCCCTGAAATGTATTAGTGGTATCTGAGCTGCTACTATAGGTGTGATAATTATCTATGCCTTTGGGTATGCTGTGCAACTGCTTAGACGGGAAAACTTGATAAATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTGTGGTATTCCGCAGGGCATGCCTGTTCGAGCGTCATTTAACCACTCAAGCCTAGCTTGGTATTGGGGCACGCGGTCTCGCGGCCCTTAAAATCAGTGGCGGCGCCGGTGGGCTCTAAGCGTAGTACATACTCCCGCTATAGAGTTCCCTCGGTGGCTCGCCAGAACCCCTAATTTTTACAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 3
## S2 0
## S3 0
## S4 0
## S5 1
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTGTGGTATTCCGCAGGGCATGCCTGTTCGAGCGTCATTACAACCAAAATTAAGCCCAGCTTAGTGTTGGGGTTCGCAGTTCTGCGGCCCTTAATATCAGTGGCGGTGCCTGTAGGCTCTGAGCGTAGTAATTCTTCTCGCTATAGAACCCTATAAGTGTCTTGCCAAAACCCCCATATTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 2
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGTATGCCTGTTTGAGTGTCATGAAAATTTCAAAGCCCTGCTGGCCTTATACCAAAGTCAGTCAAGGCGTTTGGATTTGTGGAGGTTGCTGGACAGTCTAACTATCAGCTCTTCTGAAATTCATTAGCGAAACTTTTGTTACTGGCTATATTCTATGGTATGATAATTATCTATACCACTTGAATGTCCAGGCTTTCTTTATTGAAAGAGTTTTGCTCCCAAATTGTCTTGGTTCAAGACAAATTATCGAACATTTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 4
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCTAGTATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACTATCAAGCCCTAGGCTTGTGTTGGAGCCCTGCGGCTGCCCGCTGCCCGCAGCCTCCTAAAAACAGTGGCGGGCTCACTATCACACCGAGTGCAGTAGTTTACTCTTCGCTCAGGACGTGTAGCGAGTTCCAGCCGTGAAACCCCTCAATTTTCAAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 11
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCCTTGCTTGGTCTTGGGCTCCGCTGCTTCCCCGGCGGGCCTTAAAATCAGTGGCGGCGCCGTCGGGCCCTGAGCGTAGTACATCTTCTCGCTACGGGGTGTCGATGGACGCTGGCTATCAACCTCCTTACTCTCTAAGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 8
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCACTCCTTGGTATTCCGAGGAGTATGCCTGTTTGAGTGTCATGAAATCATCAACCTCATAGTTTTATTACCCTGAGGCTTGGACTTGGAAGCTGCTGGCTTATGTCGGCTCTTCTTAAATATATTAGCTGCTTGTCCGTGAGTTGGCTCAGAGTGTGATAAGTATCTACACTTGGTGGCTGCTCGCATTGGATGTGGCTTCTAATCGTCTTCGGACAATTTTTTAAGATCTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 2
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATCTTGCGCCCCTTGGCATTCCGAGGGGCACACCTGTTTGAGTGTCGTGAAATCCTCAAAACCCCTTTTCTTTTGGGATTTTTGGACTTGGAGGGTTGAATGCTTGCTTTCACTTTTGAAAGTGAGCTCCTCCCAAATGAATTAGTAGGGTCTGCTTTGGTGATCCTTGACGTGATAAGATGTTTCTACGTTTTGGATTTAGCACTGATGCCCCACTCTTAACTGTCTTATGGACGATGATGGTGCTCCGGTCGTCGCCATCTACATTGGCGAGAGGCTGGACCCACAAAAAAAAACCTTGACCTCAAATCGGGTGAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTTTGGCTATTCCGAAGGGCATGCCTGTTTGAGTATCATGAACACCTCAACTCTCATGGCTTGCCATGATGAGCTTGGACTTTGGGGGTCTTGCTGGCCTATGGTCGGCTCCTCTCAAATGAATCAGCTTGCCGGTGTTTGGTGGGATCACAGGTGTGATAACTATCTACGCTTGTGGTTTTCCACCAGGTAACCCTCAGCAATGGAGGTTCGCCGGAGCTTATAAATGTCTCTCCCCAGTGAGGACAGATTTTGACCGTTTGATCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATTGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACACCCCTCAAGCCTCGCTTGGTGTTGGGCGTCGCGGTGATCCGCGTGCCCCAAAGTGCCTCGTGCCCGGCGGGTCGTCCGTCCCCGAGCGTTGTGACATCTCAGTTCGCTCCGGTGGATGCGGCGGCCGCGCCGTTAAACCCCCCTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCAGCTTGGTATTAGGCTTCACCCGTAAGGGCGGGCTATAAAATCAGTGGCGGTGCCATTCGGCTTCAAGCGTAGTAATTCTTCTCGCTTTGGAGGACCGGTTGAGTACCTGCCAACAACCCCAATTTTATCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCATTAGTATTCTATTGGGCATGCCTGTTCGAGCGTCATTTCAACCCTTAAGCTCAGCTTAGTATTGGGAATCTACCGCGAGTGGTGCTACCTTGTAGCTACCCTGTAGTTCCTTAAAACCAACGGCGGAGTTATAGTTACCTCTGAGCGCAGTAATTTTTATCACGCTTGGGAAGTGCTATAGTTCTTGCCGATAAACCCCCCAATTTTTAATGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTGGGTGTCACCGCATTGGTGCACCTCAAAATCAGTGGCGGTGCCACCTGGCTTCTAGCGTAGTAATACTCCTCGCTATGGAGTCAGTTGTGTACTTGCCAACAACCCCTAATTTTATAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTATAACCCTCAAGCCTAGCTTGGTGTTGGAGCCTGCCTCTGGGCAGCTCTTAAAATCAGTGGCGGTGCCGTCTGGCTCTAAGCGTAGTAATTTTTCTCGCTACAGAGTCCTGGCGGTTGCTTGCCAACAACCCCAAATTTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTCCGAGCGTCATTACACCCCTCAAATTCCATTTGATGTTGGGCGACGCCCGCCGCGGCCAGCCTTAAAGCGAATGACGGTTCCCAACCAACCTCGGGCGTACAAGTATCGCGACGGGGGAGGATCGGGGGCCTGTCCCACACAGCTTCTAAATGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATAACCACTCAAGCCTGGCTTGGTGTTGGGGTACGCGATCCCGCGGCCCCTAAAATCAGTGGCGGTGCCATCTGGCTCTAAGCGTAGTAATCTTACCCTCGCTACAGAGTCCTGGTGGATGCTTGCCATAACCCCCCCTATTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATCTTGTGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGTGTCATGAAATATCATCCCATCGGGTTTCCGATGATGATGGACTTGGGTGCTGCCAAAGTGGCTCACCTTAAAAGTGTTAGCGGTTCTGAAATCGAATCTGGCGTCATAAGTTTCGCTGGCCTAGGTTGAAGGCTGCTCATAATCCGTCTTCTGACATTTTTGCTCTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCATCAAGCCCGGCTTGTGTTGGGGACCCTGCGGCTGCCCGCAGGCCCCGAAATCCAGTGGCGGGCTCGCTGTCACCCCGAGCGCAGTACACATCCTCGCTCAGGGCCGTGAGGGCGGGCTCCAGCCGTTAAAGTGACTTTACCCCACAAAACAAGGTTGACCTCGGATCAGGTAGGAGTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCAAAATTCAAGCCTGGCTTGGTGTTGGGCGTCGCACCTTGGGGCGTGCCTGAAAATTAGCGGCGGTGCGACCGGTGATCGAGCGTAGTAGTTACTCCCGCTTCGAGACACCCCGGGCGTGTCAGCCGGGCAACCACCATTTCTCCACGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 8
## S3 0
## S4 0
## S5 0
## S6 0
## GTGCGATAAGTAATGTGAATTGCAGAATTCCGTGAGTCATCGAATCTTTGAACGCAAATTGCACTTTCTGGTATTCCGGAAAGTATGCCTGTTTGAGGGTCAATTTAATAACAATCGATGTGTTGCTTTTATTAGTAATACAACGGAATTGAGTTATCTTAACCTTAGGTTTAAGAGGCTTAAAATTAATTTAATATTGTATTTAAGACGTACTTAGAATTTTCTATACGTTTCGGATGCAATAATTTTATTAGGTATGATCATATTTTTCATGATATCATACTCATAATTTTTTCAACTTTTGACCTCAAGTCAGGTAAGAATACCCGCTGAACTTAA
## S1 0
## S2 3
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCTTAGGGCATGCCTGTTCGAGCGTCACTAAAAACCCCCTCACACATATAGTGGTATTGGTAGAAGTGGATAGTACTAACACTACTTTCTCTCACTCTGCTGAAATGAATAGGCCAGAGAAGTGGACCATGGTAATAGACTCCAGGAGTATTTTAAAATGCTAAACTAGTCTTGCCCAAGTCATGTTCTGAGCTACTGGACCCCCCATTTAGTCCAAACAAAAGAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 4
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGTGTCATTAAATTCTCAACTTCAAAGAGTTTATCTCTTTTTGAAGCTTGGACTATGGAGGCTTGCCGGCAAAATATAGTCAGCTCCTCTTAAATGTATTAGCAGAACCTTTATAAAGGACTAGCTCCAGTTTGATAATTCTATCTATGCTGTTGCTTGTTAACCTTTCTGTGTTCAGCTTCCAATAGTCTTTTTTAAGACTGTCTCTATGAGGCATACACTTTTAAGCTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCACCCTTTGGAATTCCGAAGGGTATGCTCGTTTGAGTGTCATTGTACTCTCACACTCTCTGATCCTTTGGATTTGAGGGGTAGTGGACTTGGGTGTTTGCTGCTTTCCTTGTGGCTCACCTCAAATATGTTAGTGCACCTTTCAGTTGGACATAGTACGGCGTGATAAGTATCTTCGCCGGCACCTCGCAAGGGGGTGGCTGATTGGGAGCTCTGTGCTTTCAAACCGTCTTCGGACAATCTCCGACAACTTGACCTCAAATCGAGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAATAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCATCTTGCGCCTATTAGTATTCTAGTAGGCATGCCTATTCGAGCGTCGTTTCTATCTTCAACTAATTTATAGTTGGTGTTAGAAGCTAGGATCATACCCAGCTTCTTAAATTTAATAGCTATTGTATCTGGCGTTCTTCTACAAAGTAAATCTTGTCTGTTTAAGAACACATTTATATTAGCATTAATTATTATTGTATTAGTTGACCTCGAATTAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCACCTTTTGGTATTCCGAAAGGTATGCCTGTTTGAGTGTCTTGAATTATCACTTTAAAGAGTTTTTCTCTTTAAACGTGGATGTGGGTTTGCAATCTGCTCACCTAGAATGCCTTAGTGGTTCAAACTGGATCCTGATGTAATAAGTTTCATTAGGTGAAAGGAAGACTGCTTCTAATTGCCTTTGGGCTCTCTTTTAAGACTAGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTAATATATCAACCTCTTTAGCTTTTGCTTATTGAGTGTTGGATGTGGGGGTCTTTTGCTGGTCTTTAAGAGATCAGCTCCCTTCAAATGCATTAGCAGAACAACTTGTTGATCGTTCATTAGTGTGATAATTATCTACGCTATTAACGTAAAGCAGTTCAGCTTCTAACAGTCCATTGACTTGGACAATTTTTTTATTATTAATGTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCTTCTGGAATTCTAGGAGGTATGCCTGTTCGAGCGTCATTGCGACCATCAAGCACAAGCTTGGTATTGGATGTCACCTGTTTAGGTGCATCTCAAAATTAATAGTGGTACCATTCAACTTTAAGTGTAGTAATTTTCTCGCTTTAAAGCTGGAGTGGGCTACTAGAACCTTTAATTTATCAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTGGGCTCTGCCAGTCCTGGCAGGCCTCAAAGTCAGTGGCGGTGCCATCCGGCTCCAAGCGTAGTAACTCTTCTCGCTTCGGAGGCCGCGGTGCGTGCTAGCCAGCAACCCCCAATTTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGCATTCCGGAGGGTATGCCTGTTCGAGCGTCATTACAGCTATCAAGCACTGCTTGGAGTTGGGCGTCGCTGGCTCACAGCGCGTCCTAAAATCAGTGGCGGTACCATCTGGCTCCGAGCGTAGTAATCTCTCTCGCTTTGGAGCCCGGATAGGGCCTGCCAGAAGCCAATTCTACCGTAGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCACCGTATCAAGCTTGGCTTGTTGTTGGGCCCTTTGTCACCTGGTGACAGGTCCAAAAGACAATAACCGGTGTCATACAGACTCTATATGCAACGAGCTTATAACAGCATGCATCTGGCGGTCGTGTGGCCCGGTTTTCCCTTTGATAAATAAATTTCTCAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGGTGGTATTCCGCCGGGCATGCCTATTCGAGCGTCATTACAACCCTCACGCCCCGCGTGGTCTTGGGCCGAGCCCCCCGGGCTGGCCTCAAAAGCAGTGGCGGTGCCTCTGGGTCCTGAGCGTAGTAACACTTCCGCTACAGGGCTCCCGAGCGTGCTGGCCGAACCCCAACCCTTCAGGTTGACCTCGGATTAGGTAGGGATACCCGCTGAACTTAA
## S1 6
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGTGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCCTAGTATTCTGGGGAGCATGTCTGTTTGAGTGTCATGAACTCTTCAACCCTCCAGTTTCTTGTAGACTGATTGGTGTTTGGATTTTGAATGCTGCTGGTCTTCGGACGTAGCTCATTCGTAATATATTAGCATCTCTAATTCGAATTCGGATTGACTCAGTGTAATAGACTATTCGCTGAGGACACTTTTATTAGTGGCCGAATAAGATAATTGTAGAAGCTTCGAACCTCTATAGTCAACTTTAGATTAGACCTCAGATCAGGCAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 2
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGCATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCGAGCTCCCTTTGGGGAGCCCGGCGTTGGGGACCGGCAGCTACCGCCGACCCCGAAATACAGTGGCGGCCCGTCACGGCGACCTCTGCGTAGTAATCCAACCTCGCACCGGGAACCCGACGCGGCCACGCCGTAAAACACCCCAATTCTGAACGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 4
## S6 0
## CATGGTTCTACAGATGAAGAACGTAGCGAAATGCGAAACGTAGTGTGAATTGCAGAGTCGTGAATCATCGAGTTTTTGAACGCACATTGCGCCTGTTGGCAATCCGACAGGCATGCCTGTGTGAGGGTGATCACCATGTCGTGTTTACCATGGTAATGACTGCTCCGATGGACAGTTGAAACAAGTCAGTGTGATAATTGTCACTCAACATAATACAATATGAACACCTCACATCATGCAAGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAGACCAAGTCTC
## S1 0
## S2 0
## S3 0
## S4 0
## S5 1
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTGGCATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCAACCATCAAGCCTGGCTTGTCGTTGGACCCCTTTACCGCTGAAATATGTGGTAGGTCCGAAAGATAATGACGGCGTCGTGTTTGACCCTAGATGCAACGAGCTTTTTATAGCACGCATTGAAGTGGTCAGCCGACCCGGTCTTTAACCATCATTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 10
## GCGCGATAGGTTTTGTGAATTGCAGAATTGTGAATCATCGAATTTTTTGAACGCACATTGCACCCATTGGTATTCCGATGGCTACACTTGTTTGAGCGTCATTTCATTCTCCTATATGGGTTTTTAGCTTGATTAAATCTCGCTGAATTATAGTGGTGTCGCTACCAGACTACAACGTGATAGATATTTCGTTGGATGTGACTGGGATTGCTCACCTTAAAAGTTATGTATAGACCTCAAATCAAGCAGGACTACCCCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTTTGCTTGGTCTTGGGCTCTGCCAGCAACGGCTTGCCTTAAAATCAGTGGCGGTGACATTGAGCTCTACGCGTAGTAATTAAACCCTCGCTTTTGAGTCTTGATGGATGCTTGCCAGCAACCCCTAATTTTTTTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCACGCCTGGCGTGGTATTGGGCTCGCCCTTCGGGGCCTGCCTCAAAGTCAGTGGCGGCACCGTTTGACCCCTAAGCGTAGTAATACTCGTCGCTTTTGGTGTCTAGCGGAGGCCTGCCAGCAACCCCCATTTTACCAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTAATACCAATCTAGCCCAGCTAGGTGTTGGACTTCGCCCCCGGGCGGGTCTTAAAACCAGTGGCGGTGCTTCTCAGCTCTACGCGTAGTAATTTTTCTCGCTATAGGGACTGTGGAGATGCTTGCCAGCAACCTCAAATTTTCTAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCACCCTCTGGTATTCCGGAGGGTATGCCTGTTTGAGTGTCATGAAGCATCAACCCCGACGGGAGACCGTCTCGGTTGGATTTGGGTGTCTGCCGTCCGCGGCTCGCCTTAAAAGCATTAGTGGTCTGGCCTTGACTCCGGCGTATTAGGTTCCGTCGGCCGAGTGGTCATCTGCTTACAACCCGCCCTCGGGCAACTTCTTTGACTTGACCTCAGATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTATTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTCGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTATCGACCATCAAGCACAGCTTGGCATTGGGCCTTCGTCCCCGCAGTGGACGGGCCCCAAAGACCTCGGCGGCGGCGCAGTGGCCTCAAGCGTAGTAGAAATATCCCGCTTACAGAGGCCTCGCGACGCCCGCTCCGAAAATCCATCACCTTAAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## GTGCGAAAAGTGATGCGATTTGCATGCCCTGTGAGTCATCGAATCTTTGAACGCATATGGCACCCTCTGGCGCATGCCTGGGGGTATGCCTGTTTGAGAATCATTTATGCCATTCTGGCTGGTGCGCATTTGTGCATCGTCAGAGACCTGGGCCTTCACCGCTGATCGCCCCGAATGCAGCGTGCGCGTGTCGTGATAGTCATTGGATTGATCGACGTGTTGCACTGCCCATATTTTGCCAGTGATCTCAAATCAGGCAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTTGAACCTTCTCAAGCCCTCTCTTTGAGAGTGTGGTGGGCTTGGAAATGGGGGAAGGTTGCAGGACTGCAAAGTCCTGCTCCCCTGAAATGCATCAGCGGCGTCTTGGCAGACGACCCCAGGGTGTGATACGAACATCAGCGCCATGGGGCTGCAGATTTGCCGCCGCTTTTCCAATCATTTTGACCAACTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTTAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCTAGTATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCGCTGTTCGCTTGGTGTTGGGGCTATTTACAGATTGTATCTGTAAGCCCTGAAAATCAGTGGCGGGCTCGCTAGCATCCCGAGCGCAGTAATTTTACTTTCTCGCTTTGGGCGTGCGGCGGGTTTTTTCCCAGCCATTAAACACTCCTATTTTTAAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## GGTTCTACAGATGAAGAACGCAGCAAAATGCGAAAAGTAGTGTGAATTGCAGTCGTGAATCATCGAATTTTTGAACGCACATTGCGCCTGTTGGTATTCCGACAGGCATGCCTGTTTGAGGGATAATATATTCATATTTATGACGTTGACTGATCCAGTGGACAGTTGAAAGACAGACCATGATACAGCGTGATAGTTGTTGTCACTGTAGCATCCAAAACTTGAACCTCAAATCAAGCAAGATTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAGACCAAGT
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGATTTCAGTGAACCATCGAATCTTTGAACGCACCTTGCGCTCTTTGGTATTCCGAAGAGCATGCCTGTTTGAGTGTCATGAAAATATCAACCTTGACTTGGGTTTTGTGCTCGAGTCTTGGCTTGGATGTGGGTGCTTGCCGCTTTAACGAGCGGCTCACCTTAAATGTATTAGCTGGATCTGTCTTTGAGACTTGGTTTGACTTGGCGTAATAAGTATTTCGCTAAGGACATTCTTCGGAATGGCCTCGTTTCTGGGACGCTTGTCCGCTTTCCAATACAAGTTCCATTCGTGGACATGACTTTTTATTATCTGGCCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTGTCAAAATCTCAAGCCTGGCTTGGTGTTGGGCTCTCGCTCCTCTCCCACAAGTGTAGGGGGTTATGGGGGGCGTGCCTGAAAATCATCGGCGGTGCCTCGATGGCCTCAAGCGTAGTGGACTACTCGCGCTTTGGAGCGTCCGTCGTAGGCTCACTAGCCTGGATGTGTCATTATAAAAGAAGCTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGCATTCCGAAGGGCATGCCTGTTCGAGCGTCATGATCAATCTCAAGCCTAGCTTGGTGTTGGGTTTTCGTCTCCTCCCTGTCTGGGTGGTGACGTGCCTGAAAGTCAATGGCGGTGCCTCAATAGACTCAAGCGTAGCAGACTAACTCTCGCTTTGGAGAACTTTTGTGGGTACTGCCAAACAAAACAATCAAAGTTGGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCTTCTGGTATTCCAGAGGGTATGCCTGTTCGAGCGTCATTACAACCCTCAAGCACTGCTTGGTATTAGATGTCACCATTGTGGTGCATCCCAAAAGTATTAGCATGTAGCATTTAGCTTCTAGTGTAGTAAATTTCTCGCTTTGGAGTTAACTGTCTAATTGCTAGAACCCTTAATTTGTCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 3
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCATGGTATTCCATGGAGCATGCCTGTTTGAGTGTCACGTAAACCATCGCCCGTGGTGATTTCAATGTCGCCTCGGGAGGACTTGGATTCTGCCGTAGTTGGCTCATCCTGAAATGAATTAGCTCGCGTCGTTTAGAGTGCGCAGCGCGGTGTGATAATTATCTTCATCGTTGCCTTATGCCTCTTCCATGTGTGTGGCTTACAGCCGTCCCCCGAGGACAACCCCATTTCAAGCTTTGGCCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 6
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATAAGTAATGTGAATTGCAGATTTTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTGAATTCTCAACCATGCCTTGATTGATTTCGAGGCATGGCTTGGACTTGGGGGTTTGCTGGCGGCGAAAGCTGTCGGCTCTCCTGAAATGCATTAGCAATGGACAGCAAGTCTGATGTGCACGGCCTTGACGTGATAATGATCGTCGTCGCTGGAGCATTGGACGAGCATGAATGAGTCCGTTTGCTTCCAATCCTTGACTTGGGACTTTTTCTTTCGAGCGAAGCTTTAGCTACTAGTTGGTCGTGAGGCCGACGAACGCAAGGCTGGCTTGGAGGTAGAAGGACCCTCGTCTTCCTTGACAACTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 7
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCATGGGGCATGCCTGTTCGAGCGTCATTTGTACCTTCAAGCATTGCTTGGTGTTGGGTGTTTGTCTCGCCTTTGCGTGTAGACTCGCCTTAAAACAATTGGCAGCCGGCGTATTGATTTCGGAGCGCAGTACATCTCGCGCTTTGCACTCATAACGACGACGTCCAAAAGTACATTTTAACACTCTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 2
## S6 0
## TCGCGAAATGTCAGGCAAACTGCAGAATATTGTGAGTCTGGTAATTTTTGAATGCACCTTGCAAATGTATCTACTCGATACCTTTACATTTGAATGAGTTGTCGAATATTTCACCGTGCACACACCTAGTGTGTGCGCAGTGCCGTTGCCGGCAAGTGCGTGCGTCCGCGCGCGCGCTTGCCAGTTAAATGCGCGCAAGTATGGGCGCGTCAAGGCGCCATCTATAGAGTCTCGCAAGCGACAAAGTACGATGACGCTTCCGCGCGCACCCTTGCAAACCAAATTGTGCACACTCATTTCAAGTGAGATTACCCGCTAAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 2
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCACCCTTTGGCATTCCGAAGGGTATGCTCGTTTGAGTGTCATTGTACTCTCACACTTCTGATCCTTTGGATTTGGAGCGGTGGACTTGGGTGTTGCTGCTTTGCGGTGGCTCACCTCTAATGCATTCGTGCAACTCTTGGTTGACCATAGTACGGCGTGATAAGTATCTTCGCCGGCACCTCACAAGAGGGTGGCTGATTGAGAGCTCTGTGCTTTCAAACCGTCCTTGGACAATGACTGACAACTTGACCTCAAATCGAGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCACGCCTGTTCGAGTGTCTTGAAATTCTCAAGCTGGATAGCTTTATTGTTATCTCAGTTTGGATTTGGGCTCTGCCGTCTCCTCTTTTGTGAACGGCTGGCCTTAAAAGTATTAGCTGACCCTTGTTGTGGGATTGGTTCTACTCAGTGTGATAATTTTATGATCGCTGAGGACATCTTTCGGGATGGCCAGTTTTGCCTAGGATCGCTTCTAATCCTGGTATTTGGAGTCCGCTCCTTATCCTTTAAACTTTTCAACTTTGACCTCGAATCAGGCGGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTGGGCATTCCTAGGGGCATGCCTGTTCGAGCGTCATTGTAAAAATCTCAAGCTCAGCTTGGTGTTGGGTGTTTGCCCCTCCCCCCTACAAGGGGGGAGAGGCACGCCTGAAAATCAGTGGCGGTGCCATGATGGCCTCAAGCGTAGTAGACTCTCTCTCGCTTTGGATGGTTCATTGTGCGGTTCACCTGTCTAACCTAAAATCTCAAAAAGGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATGATCAAATCTCAAGCCTAGCTTGGAATTGGGCTTTCGTCTCCTGCCATTGCGGCTAATTGAGACGTGCCTAAAAAGAAATGGCAATACCTCAATGGACTCTAGCGTAGCAGACTGACTTGCGCTTGGAGGGCTGTTTGTGGGTTTTTGCTATAAAATAATCAAGTTGGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCTCTGGTATTCCGGAGGGTATGCCTGTTTGAGTGTCATTAACACCTCAACACAGACAGGTTTTACCACCTGTTCTGGTGCTTGGATGTTGAAGGCTTGCTGGTGTTTTCACCAGCTCCTTTTAAATGCATTAGCAGATCCAACAGAGCTGGCTTCAGTCTGATAAACCTCTTATCTGTATTGCTGTCGCTAGTGAATTTTGTTGTGTTCAGCTTCAAATTGTCCAGTTTCATTGGACTGTCTCCTTGGAGGCATACACCCTGACACTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAGCCCCCCCGGGGGCCCGGCGTTGGGGATCGGCACCCCGCCCTCAAAAGGCGGGAGCCGGCCCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGCTTGCACACTCGCACCGGGAGCGCGGCGCGGCCACGCCGTGAAACACACCAAACTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGTGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCGCTGGTATTCCGGCGAGCATGTCTGTTTGAGTGTCATGAACTCTTCAACTTCTTGTTCCTAGTGAACAATGAATGCTTGGATTCTGGGGGCTGCAGTGTATTCTGCTCCCCCGTAAAGTATTAGCATATCTAATTCGAACCTCGGATTGACTCAATGTAATAGACTATTCATTGAGGACGAGCGTTGTAAAAGTGATAGCTTCAAACCTACCCAGTCAACCTTTTAGACCTCAGATCAGGCAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAAACTAGTGAATCATTGAATCTTTGAACGCACATTGCGCCTCCTGGTATTCCGGGAGGCATGCCTGTTCGAGCGTCATTAAAAACCACTCAAGCTCTTTTGCTTGGTCATGGAAGAAGAGTTTGCTTGCAATCTCTCCTCCGAAATTCAAAGGCGGAAAGTCTCAGAGCCCCGGCGTAGTAATAATATTTCGCTTGGAACTTGAAATGATCCTGCCCTTAACCCCCAATTTATCTAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGCCATTCCGAGGGGCATGCCTGTTTGAGTATCATGAACACCTCAACTCTCGCGGTTTGCCGTGACGAGCTTGGACTTTGGGGGTTTTGCCGGCCTGTGGTTGGCTCCTCTCAAATGAATCAGCTTGCCAGCGTTTTGGCGGCGTCATGGGTGTGATAACTATCTACGCTCGTGATCGTCTGCCGAGTGACCTTCAGTGATGGAGGTTCGCTGAAGCTTACAAACGTCTCTCCTCAGCGAAGACAGCTTTTTGAACGTTCGATCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCTTCTGGTATTCCAGAGGGTATGCCTGTTCGAGCGTCATTACAACCCTCAAGCACTGCTTGGTATTGGATGTCACTATTGTGGTGCATCTCAAAAGTATTAGTATTAGCATTCAGCTTCTAGTGCAGTATATATCTCGCTTTGGAGTCTTTGAGTGTCTAATTGCTAGAACCCCTAATTTATCAAAAGGTTGACCTCGGATCAGGTAAGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCTGAGGAGCATGCCTGTTTGAGTGTCATTAATATATCAACCTCTACAACTTTTGTTTGTTGAGTTGTTGGATGTGGGGGCTCTTGCTGGCTTCTCTGAGGTCAGCTCCCCTGAAATATATTAGCGGAACGATCCCCGTTCATTGGTGTGATAACTATCTACGCTATTGACGTGAAGTAGTTTGGCTTATAACAGTCCATTGACTTGGACAAATTTTCATTAATGTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCCGTGAATCATTAAATCTTTGAACGCACATTGCGCTTTCTGGTATTCCGGAGAGCATGTCTGTTTGAGAATCAGTTTAACTCTCACCCGCGTAATAAATTTCGGTTTTTTACACGCGGTGGACTTGAGCCATCCCTGGTAAATTTTTACCCGGGCGGTTCTAAACTGATTATAGCGCGTCTTGTTTTCCGCTTGACGCCCCAAAAGCATTATTGATAGAGGCAACTCTTGTTTTCGTGCTGATAAAGGGCTAGGAGAAGATTACCAGCTAAAAAATCTTGTACACGGGGGCATTACGCCTCTAAGACTTGATCTCAAATCAGACAAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCTATTAGTATTCTAGTAGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTCTGCTTGGTGTTGGGGCGTCTGCAGCTCCGGCTGCAGGCCCTGAAATCTAGTGGCGGGCTCGCTACAACTCCGAGCGTAGTAATATCTCTCGCTTTGGAAGTGTAGCGGGTTTCCGGCCGTTAAACCCCCAAAATTCTGAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATATTGCACCCTCTGGTCCATTCCAGAGGGTATGCCTGTTTGAGTGTCATTAATGTATCAAACCACCAAGCTTGCTTGGTTGGTCTTGGATGTTGAGGGTTGCTGGGGTTATAATGATCAGCTCCCTTTAAATGCATTAGCTTGGAATGTATAAGCCATTTTAGCTTAGGCTGATATGAATACAGCGTATTAAATGCTTTTGCTAAAGTGTAGCTTGTCTGGGCTTATAACTGTCTCTAGCTGAGACTGTCTTTTGACATTGTTAAATCATGATCATGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 6
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTGTGGTATTCCGCAGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTCTGCTTGGTGTTGGGCCCCGCCCCCGTGGCCGGCCCCAAAGTCAGTGGCGGTGCCGTCCGGCTCTAAGCGTAGTACATCTCTCGCTCTAGGGTCCCGCGGTGGCCTGCCAGAACCCCAACTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 4
## S2 0
## S3 0
## S4 1
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCTGAGGAGCATGCCTGTTTGAGTGTCATGAAATTCTCAACCTTTTAAGCTTTTTGTTTAAAGCTTTTATTAAGGCTTGGATTCTGGGAGTTTGCTGGCTTTTTATTGAGTTAGCTCTTCTTAAATGCATTAGCAAGATCACCGCTGAATCAATCTTTGATATGATAATTATATCTATATTATTGATGGATAAGCATATTTTCCTTCAAGGAAAAGATATAGCTTCCAACCGTCTTCAGAGACAGTCTGATACATCATTTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 1
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTCTTGGGCGTCACCGGTTTCGGTGTGCTCTAAAATCAGTGGCGGCGCCGTCTGGCTCTAAGCGTAGTACATGCTCTCGCTATGGACGTCCGGCGGATGCTTGCCAGCAACCCATCATTTATCAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCACACCTGTCCGAGCGCCATTTCAACCCTCAAGCTTCGCTTGGTGATGGGGACCGCCCGCGGGCGGCCCCGAAACACGTGGGCGCCGACGGCCGGCCCTGAGCGTAGCAAAACCCTCGCTCGGGACCCCGGCCGGCGGCCGCCTCGACCTCAAACTCACAAGGTTGGCCTCGGATCAGGTGGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTATGCTTGGTCTTGGGCACTGCCGGTAACGGCTTGCCTTAAAATCAGTGGCGGTGCCATTCGAGCTCTACGCGTAGTAATTAAACCCTCGCGATTGAGTCTTGGTGGATGCTTGCCAGCAACCCTCAATTTTTTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACTCTCTGGTATTCCGGAGAGTATGCTTGTTTGAGTATCTGTAAACATCTCAAATCTCTTTGCGCTTGCGCATAAGAGACTTGGATTTGAGCGATCCCGACTCCTTCGACAGAAAGAGGAGGGTCGCTTGAAATGCAGGTGCAGCTGGACATTCTTCTGAGCTAAAAGCATATCTATTTAGTCCCGTCAAACGGATTATTACTTTTGCTTCAGCTAATACAAAGGTCGAATGAACTACCAACGCTGATTGCAGGAAAACTCGTTTTTTTACTAAAACTTGTAAACTCGATCTCAAATCAAGTAAGACTACCCGCTGAACTTA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAAAGGGCATGCCTGTTCGAGCGTCATTTAAAATCTTCGAGCCTAGGCTTGGTGTTGGGCTTCATCTGTGGATGGGCCTCAAAGTTAGCGGCGGTGCCCTCCCGTGCCTCAAGCATAGCAGAAATTCCTCGCTTTGGAGACTCGGTCTTGGGTATTTGCCTTACCAATTTTAAAGAGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ACGCGATAGGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCTTTTGGTATTCCGAGAGGCATGTCTGTTTGAGCGTCAGTACACACCTCCTTCGTGACTTTTTTGTCGCAGAAGGGTTCTGGGGTCAGGGTCGGCAACGGCTCTCCCTCAATACAGTACGCTTGCGGGCTCCCACACAGATGTCAACGTAGTAAACTTAACGCTCGCTGATGAATCTTGTGGAGGGCTCTGCCTGATAACAAACTTTCTATGGTTTGACCTCAGATCAGACAAGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCGTGAAATTCTCAACCTTTCAAGCTTTTGTTGAAGCTAGTCAGGCTTGGATTCTGGGAGTGGTGCTGGTTTTAACAAATCAGCTCTCCTTAAATGTATTAGCAAGATCAATCGCTGAATCAACTTTGATATGATAATTATCTGTATCTTTGATGAAACGGTGTTTTCCTCTTTTTTAAAGGGAAAGATCTGGCTTCTTACCGTCTTTTCGGAGACAGTTCTTTTGAATCATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGATTTCAGTGAATCATCGAATCTTTGAACGCACCTTGCACTCTTTGGTATTCCGAAGAGTATGCCTGTTTGAGTGTCATTAAATTCTCAAACCTCTTTGGCTTTATCGGTCGAAAAGGCTTTGGATGTGGGAGTTGCTGGAACTAGCCTTTCAGCTCTTCTTAAATGTATTAGCGGGTTACTGATCTCCGCTACTTGGTGTTGATAGTCTATCTTACGCCTTGTAGTTGAATCAACTTATGTAGCTGCTTCTAATCGTCGACTTGGTTGACAAATTTACTTTGACTATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCTCTGGTATTCCGGAGAGCACGTCTGTTCGAGTGTCATGAAACTCTCAAACAAAGATGGCTTTGTTGCTGTCTTTGTTTGGATTTGGACCTTGCTGTGTCAATGCGGCTGGTCTTAAATATATTAGCTGGTCCTCACGAAAGGTTTGGTTCTACTCAGCGTGATAATTATCTAACGTTGAGGACGGTCTTAGGACTGGCCAGAGCTATCTTTGGATTGCTTCTAATTGTCCTTGGGGACAAATTATTCAATATCTGACCTCGAATCAGGTGGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCGTGGCATTCCGCGGGGCATGCCTGTTCGAGCGTCATTATAAACCCAATCACGCCTGGCGTGGTCTTGGGGCTGGCAGCTCTGCCGCCCCTAAAGACAGTGGCAGCGCCGTAGGGCTCTTAGCGTAGTAATACTCCTCGCTATAGGGTCTCGCGGCCGCCCGCCAGCAACCCCATCTTTCTCGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGCATCATTAAATTCTCAACTTTAAAAATTTTTGTAACAAAGTTTTTAAAGCTTGGACTTGGAGTGTGCTGGCTTGACCAAAAGTCGGCTCCTCTTAAATGAATCAGCAAAACCCTACATTGGGGGATAGGCTCTGGTTTGATAATTATCTATGCCATTGCTGTGAAACCTTTGTACTATGTGTTGGATTTGCTTCTAATGGTCGAAAGACAATTTATTTTGAATTTGATCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 1
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCTTCTGGTTTTCCAGAGGGCATGCCTGTTTGAGTGTCATTAATATCTCAACGCAATTCATGGTTTGAACATGTGCTTGGATGTTGAAGGTTTTGCTGATGTCTGTCATCAGCTCCTTTGAAATGCATTAGCAAATCTTAAGAAGATCAGCTTAGGCTTGATAATTATACATTTGTCTTTGCTGAGACACTATGGATCTTGCTTTCAACTTGTCCATCTCTGGACTGTCTCTCGAGGCAAACGCATAACTCTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 7
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATATTGCGCTCTCTGGTATTCCGGAGAGCATGCTTGTTTGAGTATCAGTAAACACCTCAACTCCCCTTTCTTTTTTGAAATGGGAGCTGGACTTGAGTGATCCCAACGCTTTTCTCACCGAAAAGCGGCGGGTTACTTGAAATGCAGGTGCAGCTGGACTTTTCTCTGAGCTATAAGCATATCTATTTAGTCTGCCTAAAAAACAGATTATTACCTTTGCTGCAGCTAACATAAAGGAGATTAGTTCTTGTGCTGACTGATGCAGGATTCACAGAGACAGCTTCGGCTGGCCTTTGTAAACTCGATCTCAAATCAAGTAAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 4
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCACCCTTTGGTATTCCGAAGGGTATGCCTGTTTGAGTGTCATTAAATTCTCAACTTCAAAAGACTTTGTTGTATTTTGAAGCTTGGACTGTGGAGGCTTTGCCGGCGTCATTGTCAGCTCCTCTTGAATGCATTAGCGGAACTTTTTAAAGACTAAGCTTTGGTTTGATAATATATCTATGCCGTCGCTGTGACTTTGTGTTCAGCTCACAATTGTCTTCGGACTGTCTCTTTCGAGGCATGAATACATAAGGTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 7
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTCTGCTTGGTATTGGGCCTTCGCCAGTAAAATGGCGGGTCTTAAAATCAGTGTGCGGTGCCGTTGGGTCCTGAGCGTAGTAATTCTTCTCGCTACAGGTACCCCGCGTGCCCCCGCCATTAAACCCCCAATTTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 3
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCACCTTTTGGTATTCCGAAAGGTATGCCTGTTTGAGTGTCTTGAAACCTCACACTCGAGAGTTTTTTTATCTTTCGAGAGGTGGATTTGGGTGTTGCAATTCTGCTCACCTTGAATGCCTTAGTGATTCCAACTGTAGCCTGATGTAATAAGTTTCATCTGGTCAAGGGGAAGATTGCTTCGAACGGCCTTTATCGGCACTATTTAAGACTAGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 3
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTTCTTGGTATTCCGAGAAGCATGCCTGTTTGAGTGTCATGAAATTCTCAATCTTTATTGGCTTTTGACAAAAAGCCGATGGAAGGTTTGGATCATTGAGAGTTGCTGAATTTTAAACAAAATTTCAGCTCTTTTCAAATATATTAGCAAAAATCATGCCTGATCATTGATGGTATAATAAATCATCTATACTATTGATATTGGATTTCTTTTTGCTTCTAACTGTCTTTTTAAAGACAAACAAAATGACAACTTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCACTCAAGCCTAGCTTGGTGTTGGGGTTCGCCTTTTGGTGGCCCTTAAAACCAGTGGCGGTGCCGGCTGGCTCTAAGCGTAGTAATTCTTCTCGCTATAGGGTCCTGGCGGTGCTCGCCATTAACCTCAAATTTTACTAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTAACACCACTCAAGCTCTGCTTGGTATTGGGCCGCGCCAGTGATGGCGAGCCCTAAACTCAGTGGCGGCGCCGTTGGGTCCCGAGCGTAGTAAATCTCTCGCTATGGGTCCTGGCGGTCGACGACCATCAACCCCAATCTTTTTAAGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATCCGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTCGAGCGTCATTATCACCCCTCAAGCTCCGGCTTGGTGTTGGGCCTGGTCTTTCAGTAGACCGGCCTCAAAGATAGTGACGGCGTCCCAGATGGAACCGGTACGAGGAGCTTCTAATCAAGCATGTACGCGGCGTGAATCTGCGGGCGGCGGTCTTTAACCACTACTCTTCGGAGTACTTTTTTTAAGTGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCTTGGAGCATGCCTGTTTGAGTGTCTTGAATATCTCTGCTCCTCAATTTTTTAATTAAAGTTGGTTTGGAGTCAGGTCCTTGGGCTTGCTTGCGCTTTGTTACTAAAGACAAGCTTGCCTTAAATGTATTAGCTGGATTTCAGTAGAGTTGTTAAGTAACATTGAAAACTCTCGTAATACGGAATCTGCTCATAACTTTAGCCGTCTTTATTGACGCATATTCATTTTCTTTGGCCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTAAGTATCTCAAAAGCTTATGCCTATTTTGTGGCGTGAGACTTTTGGACAATGGGAGTTGCCGGTCACTGATGAAAGTGGTGGGCTCTTCTGAAAAGTATTAGTTGAGGAACTTTGCACTCTATTGGTGTGATAGAATATCTATGCCAGGAGATGCTTTATGATCCTCTGCTGTCTAACTGTCTTCATTGGACAAACATGATGAACTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCCCCTGGCATTCTGGGGGGTATGCCTGTTCGAGCATCATTACAACCCTCAAGCCTCGCTTGGTGTTGGGTGCCACCCGCTATAGGGTGCACCTCAAAATCAGTGGCGGTGCGGCCTAGCTTCAAGCGTAGTAATTCTTCTCGCTCCGGAGCCTAGGCGGCATATCTGCCAACAACCTCAAACACATCAAGGTTGATCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTTTGGCTATTCCGAAGGGCATGCCTGTTTGAGTATCATGAACTCCTCAACTCCTCATGGTTTGCCATGATGAGCTTGGACCTTGGGGGTCTTGCTGGCCCTACGGTTGGCTCCCCTCAAATGGATCAGCTTGCCAGTGTTTGGTGGCATCACAGGTGTGATAAATATCTACGCTTGCGGTTTTCCACCATGTAACCTTCTGCAATGAAGGTGTGCTCTGGAGCTCATAAATGTCCCTCCTCAGCGAGGACGGCTTTTGAACGTTTGATCTCAAATCAGGTAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATATTGCACCCTCTGGTATTCCGGAGGGTATGCCTGTTTGAGTGTCATTAATCATCTCAAGGCAAAAAGTCTTCTTTTTGCAATTGGATGTTGAAGGTCTTGCCGGCTTTCTTGGTTGGCTCCTTTTAAATACATTAGCAGGACTTTTGTGCAGGACTTGCTCCAGTTTGATACATTGCTATCTGAGCTGTTGCGCCAGCAGTGTCATTTGCTTCGAATTGTCTTCGGACTTTTGTCATCTTGACAAGAAACACCCTTGAATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCTGAGCGTCAGTTCAACCCTCACAACCGGCTTTCTTGCTGGATGTGGTGTTGGGGATCGGCTGCCTTTCTTCTAGGTGGCCGGCCCCGAAATAGAGTGGCGGTCTCGTCGTGGCCTCCTCTGCGTAGTAGCAATATCTCGCAGGCGGAGAGCGGCGTGGCTATGCCGTAAAACACCCAACTCTTCTAAGGTTGACCTCAGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCGTGTAATTATCAACTCCCTTTCTTTATTGAAGGGTGAGCTTGGACTTGGGGATTGCTGGTTTCATTATCGGCTTCCCTTGAATGCATTAGCTGGAATTTGATTCGCAGCATATATGCGGTGTGATAATGTCGTCACTGTGTAGCTCGGATTGTCTAGCTTCTAATAGTCCTTCATTGGACAATTTGATCATTTTGGCCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## GTGCGATAAGCAATGCGAATTGCAGAACCGTGAGTCATCAGATTTTTGAACGCAACTGGCGCCGGCTGGAACTCCAGTCGGCATGCTTGTTTCAGTGTCTTGTTTTCTCCTCACCTAAACCTTAATGCGAGAGATACCCTTCTCTTGCCAAACAGGAAAGCACTCTGCGCTCTGCAGGCAGCTTCGTAGACTCGTTCTTGAAGCTGCGCTCAATGCTGTCGTCACATTTTTCAAAGTGTGAACTCATTGAGAGTAGAGGCTTGTAGCTTCGGCTACCAGTGCTTAGTCACACAAACTTTCTTTGCATCTGAAATCAAGCAGGATCACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTATGACCAATCCCGCAAAGGGGTCTTGGGGTCCGCGGTTCTCTGTGGCCCTTAAAATCAATGGCGGCGCCAGCGGTGTTCTCAGCGTAGTAATACTCCTCGCTCGGGAACCACGGTGGAGGCTTGCCAGCAACCTAACTATACTAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGTATTGGGTGTCACTGTCTTGGTGCACCTTAAAATCAGTGGCGGTGCCACCTGGCTTCTAGCGTAGTAATATATCTTGCTATGGAGTTCAGGTGTATGCTTGCCATCAACCCCTAATTTCTAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## CTGCGATAATTTGTGCGAACTGCAGAAACCTTGAACATAAAAGTTTTGAACGCATATTGCGGCATTGGGGTCAAACCCTTTGGCACGTCTGGTTCAGGGTCATTTTCTAAAAAGCAGAAGAAGCTTTTTATTTTGGATTGCTATTATTATGCATTGAAATTTTAATTGTGTTTTGACAGGATATTTCATGATTTTGTTGCAATATTCTTCAATTCTACAATAAATTTTTTGACTTGAACTCAGTCTAGAGCACCCGCTGAACTTAA
## S1 2
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTTAGTGAATCATCGAATCTTTGAACGCACATTGCGCCTCCTGGTATTCCGGGAGGCACACCTGTTCGAGCGCCGTTTTCATTCTGAAGGAAACTTCTTGTTGATGGATCCCGTCTGGGACCGTCGAAACTGATTGACGCCGGTTGGTGCAACATACGCAGCGTAATGCGAAAATGTTTACCGACTGAAAGTCTGTTCGAAAAGTTTGGCCTCGAATCAGGTGGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 4
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTAAATTCTCAACCCCTCTTCAGTTTTCTGAAGGGGTGGATTTGGATGGTGGAGGTGTTGCAGGCTTGATCAAAGTCAGCTCCTCTTAAATGCATTAGCAGAACCCTTGCATTGACTTGGTTTCTGGTGTGATAAATTATCTGCGCCATTCCAGTCGATCAAAAAGTCATGGTTTAGCTTCTAATCTGTTTATTGTCCTTGAAGGATTAAAAACACTTTTTAACTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTCCGAGCGTCATTATCAACCCTCAAGCCCGGCTTGTCATTGGGCCCCGTCACGGTCAAGGTGACAGTCCCGAAAGGTAATGGCGGTGTCGTGTTTGACCCTAGATGCAACGAGCTTTTACAGCACGCGTCGACGCGGTCTACCGGCCCGGTCTTCAAATCTATTTTTCTAAGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGCGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCTCTCTGGTATTCCGGAGAGCACGCCTGTTCGAGCGCCATTTCACCCCCCAAGCAGTGCTTGATGTTGGAGGCCGCGGAGAGATCCGCGCCTCTGAAACTTGTGAGGCGCCGTCCGTCGAGCACCAGCGTGGCAAGCAAACTTCGCTGGGATGTGTTCGGATGGGTGGCAGCCGCGACGTACCAATTTTTTGGGATTTTCCCGAAAATCAGGTTGGCCTCGGATCAGGTGGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGCATTCCGGGGGGCATGCCCGTTCGAGCGTCATTAACACCCCTCAAGCCTGGCTTGGTATTGGGCGCCGGGACTCTCCCGCGCCCCGATGTCGCCGGCTGGACGACTCCGATCTCACAAGCGTTGTACAAATTCGCTGGCGGGACGGGCGGCCACGCCGTTAACCTCAATCAAATCGTTGACCTCGGATCGGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTTAGTGAATCATCGAATCTTTGAACGCACATTGCACCTCTCGGTAATCCTTGAGGTATACCTGTTCGAGCGCCGTTTGTGTCTAAGGCTTTGCCTTGCGTTGAAGTTTCCCGCCAGGGATTCTTCCAAACACATCGGCGCCCTCTTTGGCTTCGAAACGCAGAGAAATGCGGCGAAAGGTCGAAGAGGGTGGCCCATCTCCGAAAGCATGGCCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGTGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCGCTGGTATTCCGGCGAGCATGTCTGTTTGAGTGTCATGAACTCTTCAACTTCTTGTTCCTAGTGAACAATGAATGTTTGGATTCTGGGCGTTGTATTCGTTTACTCGCCCGTAATAAATTAGCAAATCTAATTCGAACTCGGATTGACTTGATGTAATAGACTATTCATCGAGGACGAGCTCAGCGGATAGCTTCAAATCACAAAGTCAACTTTTAGACCTCAGATCAGGCAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCTTAGGGCATGCCTGTTCGAGCGTCATTTACACCCTCAAGCACAGCTTGGTGTTGGGCGTCTGTCCCGCTTCGGCGCGGACTCGCCTCAAAACGATTGGCAGCCACTTCAGCGGCTTTGAGCGCAGCAGAATTGCGTCTCTCGTCGCGGCGGATGGCATCCAGTAAGCAACATTTATATCTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTTAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCTCGGGGCACGCCTGTTCGAGCGCCGTTTCAAACCTAAGGCTCTGCCTTGCGTTGGCGCCATCCGTCCGCGGATGCGCCGAAACACTTAGGGCGCCTTCCTGTCCCCGGCAGCGTAGCAAGTCGATCGCTGTCCGGGCCCAGCGGAGGCCGCCGGTCATCACGAGTTTGGCCTCGAATCAGGCGGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATCAATATCTCAACCACACCCGACTCGTTCGTTGGCGTGGCTTGGATTTGGGGGTTGTGCAGGCTTCTGCCCTTGCAGTCTGCTCCCCTGAAATGCATGAGTGGTCTAACCCTCCCCATCAGGTGTGATAATTATCCGCATCTGATCGGAAAAGGTCGTGGCTGCTTGTATGTCGTTGGAGTCGATAAACTTGACCTCAAATCAGGTAGGACCACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCACACCCGTTTGAGTGTCGTGAAAATCTCAACCTCCTTGGTTTCTTTTGAACGCCAAAGGAGGCTTGGACTTTGGAGGGCTTTGCTGGACTGGAAAGGCCAGCTCCTCTCAAATGAATTAGCGAGGTTCCCTTTGCCGATCCTCGACGTGTGATAAGATGCTTCCACGTCTCGGTCTTTGGCTCTGTCGCCTTTTGGGACCTGCTTCCAATCGTCTCTCGACGAGACAATGTTCGAGCTTGCCTCGACCACGTACCCTTGACCTCAAATCGGGTGAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTGTGGTATTCCGCAGGGCATGCCTGTTCGAGCGTCAGTACAACACTCAAGCTTAGCTTGGTCTTGAGCCCAGCCGGTTACGGCAGGCTTTAAAATCAGTGGCGGCGCCATTTGGTTCTAAGCGTAGTAATTTTTTTCTCGCTCTTGAGTCCAGGTGGATGCTAGCCAGCAAAACCCCCAACTTTCTAAGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATGGAATCATCAACCCTCATCTTTATTGATGAGGGCTTGGACTTGGAGGCTCGTGCCGGTTTTGAATTGAATCGGCTCCTCTTGAATGCATTAGCTTGAACCTATGCCGTATCGGCTGTTCGGTGTGATAATTGTCTACGCCGTGGCTGTGAGGCTTTAAACTCGTAGGATCAGCTTCGAACCGTCCCTATCGAACGGGACAACATCTTCATTGACCTCTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGTGCCCCTTGGTATTCCGAGGGGCACACCCGTTTGAGTGTCGTGAACATCCTCAACCTTCTTGGTTTCTCGACCGGGAAGGCTTGGATTTTGGAGGTTTTCCTTTGCTGGCCTTCCTTCGAAGCCAGCTCCTCCTAAATGGATTAGTGGGGTCCCCTTTGCCGATCCTTGACGTGATAAGTGTGCTTCTACGTCTTGGGTTTCGTGCTGCGTTGGCCTGGAACCCGCTTCCAACGGTCTTCGTTTTGAAGACAATCTTTGACCTTGACCTCAAATCGGGTGAGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGATTTTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCTTTGGTATTCCGAAGAGCATGCCTGTTTGAGGGTCATTGAAATCTTCAACCTTGTCCCCATCGTCGGGACATCGGTTGGTTTTGGGGCGTGCTGGCCCGCGGGTCGGCTCCTCTTCAAATGCAACAGCGAAAGGGTTCCTGTTGGCCTTTGACAGTCGGCGTGATAAATGTTTGCGTCGTCCGTTTCGCCGGCAGACTTTTGCTCCCAACAGTCCCCCATGGGACAAAAAGCCTTCTGATACTCTTGACCTCAAATCAGGCAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 5
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATCTTGCGCTCCCTGGTATTCTGGGGAGCACGCCTGTTCGAGTGTCATGAAACTATCAAACAAGATAGATTTATTTCTATCTCTGTTTGGATTTGGACTCTGCTGTGTCAATGCAGCTGGTCTTAAAAATATTAGCTGATTCTAGTGAAGGCTTGGTTCTACTCAGCATGATAATTATCTGATGTTGAGGACAGTCTCAGGACTGGCCAGGCTCTCTTTGGGATTGCTTCTAGTCGTCTTTGGACAATTTATTTAATATCTGACCTCGAATCAGGTGGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTCGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACACCCCTCAAGCGTAGCTTGGTATTGGGTCCTCGCCCCCCGGGGCGTGCCCGAAAAGCAGTGGCGGCTCGGTGTGACTTTAAGCGTAGTAAATTTTCATCCCGCTTTGAAAGTTCGCCCCGTGGCTTGCCAGACAACCCCATTTATTTCAATGATTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCCTAGCTTGGTATTGGGCTCCGCTGCTCACACAGCGGGCCTTAAAATCAGTGGCGGTGCCGTCGGGCTCCTGAGCGCAGTAAACATCCTCGCTTTAGGGACCAGTGGACGCTTGTCATCAAACCCAACTTTCTAAGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTCGAGCGTCATTTTGACCAACTCCTATTCTTTATAGGGTCTTGGGTTATGCCTATTAGGCAGGCCTCAAAATAAGTGGCGGTGCTATATAGCTCTACGCGTAGTAATACTTCTCGCTATAGACACTATGTAGTATCTTGCCAGCAACCCCCAACTTCTTTAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGAATTTAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTCGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTGTCACCATTCAAGCCCTGCTTGGTCTTGAGCACCGCCGGCCAGGCGTGCTCCAAATCGAGTGGTGGTGCCCACGGGAACCCGAGCGTAGTACAACCTCTCGCTCCGGGGCCCCGGGGGCTCCCCCCAGCAAAACACTCATCTGGTTGACCTCGGATCAGGTAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCATTGGTATTCCGATGGGCATGCCTGTTTGAGTGTCTTGAAATCATCAAGCCCGTTCGGTTTCACCGCCGAGCGTGGGTTGGACTTGGGGGCTTGCCGCTTCGCCGCGGCTCCCCTTAAATGCATCGGTTTCGGGTCTCTGTGGGAGTAACCCCTTGGGCGTTGTATAACCCTCGCTGTCTGGGAACTCCACGCGACGTCCGCGTCGTTGAAGGGCCTTGAAGAAACCTCGACCTGCTGTAGAGTGGCGGTCATTGTTAAATCGAAATCTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTCTTCCAGCGTCATTTCACCCCTCAAGCTCTGCTTGGTCTTGGGCCACGCCGCGCAAGCGGCGGGCCTCGAAAACAGTGGCGGCGCCATCTGGGCTCTCAGCGTAGTAATTCTTCTCGCTGTTGGGTCCCGGTGGCCGCCCGCCAACAACCCCCAACTTTTACAGTTTGACCTGGGATGAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGCTATTCCGAGGGGCATGCCTGTTTGAGTATCATGAACACCTCAACTCTCATGGTTTGCCATGATGAGCTTGGATTTTGGGGGTTTTGCTGGCCTGTGGTCAGCTCCTCTCAAATGGATCAGCTTACCAGTATTTGGTGGCGTCACAGGTGTGATAACTATCTACGCTTGTGGTTGTCTGCTGAGTAACCTTTGGTGACAGAGGTTCGCTGGAGCTTATAAATGTCTTTCCTCAGTGAAGACAGCTTTTTGACCGTTCGATCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCATTAGGCCGAGGGCACGTCTGCCTGGGCGTCACGCATCGCGTCGCCCCCAACCCCGCACTCCCTCATTGGGAGTCGAGGCGGAGGGGCGGATACTGGCCTCCCGTGTCTCACCGCGCGGTTGGCCCAAATGTGAGTCCCTGGCGACGGACGTCACGACAAGTGGTGGTTGTAAAAAGCCCTCTTCTCATGTCGTGCGGTGACCCGTCGCCAGCAAAAGCTCTAATGACCCTGTTGCGCCGTCCTCGACGCGCGCTCCGACCGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGTCTGTTCGAGCGTCATTACAACCCTCAAGCTTTGCTTGGTATTGAGCTTTGCCTGCTAAAGGCAAGCTTAAAAATCAGTGGCAATGCCTTTCAGCTTTGAGCGTAGTAAATTTTCTCGCTACGAAGTCTGGATGAGTAGTCGCCATAACCCCATATTTTTAAAGGTTGACCTCGGATCAGATAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTTTTTGAACGCACACTGCGCCCCTTGGTATTCCGGGGGGCATGCCCGTTCGAGCGTCATTTCACCAATCAAGCCTGGCTTGGTATTGGGCCACGGGGGCGACCCGGGCCTCAAAGTTCGCCGGCTGGAAAGCTCTGATCTCAGCGTCGTGATCATCAATTCGCTACGGAGAGGGGCGGTCTGCGCCGTGAAACTCATCACAGGTTGACCTCGGATCGGGTAGGAATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTAATGTGAATTGCAGATTCAGTGAATCATCGAATCTTTGAACGCATATTGCACTCTTTGGTATTCCGAAGAGTATGCCTGTTTCAGTATCATGAAAAACCTCACAAATTCAATTTTGGCTTTGTGGACTTGAGCATTTTGCGGCTTTGTTGCTGCTGGCTTAAAATATATTTCTTGGATAGCATATTATGGCTTTCGAAACTCGGCTTAATAGTTTTGGCTTTTGGTCAAATCTTTAGCTCTTTTCAAAGTCTTCAAGTTATTCAAAAGTTTTATACGAACACTTTCTCAATTTTGATCTGAAATCAGGTAGGATTACCCGCTGAACTTAA
## S1 3
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATGAAATTCTCAACCATTTCAGCTTGCTGATCTTGGCTTGGACTTGGGATCTGCTGGCTTCCAACAGGGAGTCGGCTGTCCTCAAATGCATTAGCAGGACTATGTCGATCAGCATTGGTGTGATAATTATCTACGCCGTGTGGAAAGACAAGAGTATGGTTCAGCTTCTAACCGTCCCCACCCTGGGACAATCTTCATTGACAATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 1
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGATTTTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTAAATTCTCAACCCCGAATCGATTTGTTTCGAGATCGGAGGCTTGGACTTGGAGCGTGCTGGCCCTCACCCGGGTCGGCTCCTCTTTAAATGCATCAGCGGAACTTCCTTTGGTCTTCCCGGTCATCAGTGTGATAATCATGTCTCGCTGTTGGCTTAGATCAGAGTCCGCTTACAATCGTCTTCGGACAACTTTTATACCAATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCCTGGTATTCCGGGGAGCACGCCTGTTCGAGCGTCATGAAGCTCTCAAACATATACTTTTCTTTATTGCGAAGTATATGGTTGGATTTGGACTCTGCTGTGTAACAAACGGCTGGTCCGAAAGATATTAGCTGATCCTAGTGAGACTTGGTTCTACTCGGCGTGATAATTATCTAACGTCGAGGACATCGCAAGATGGCCAGATCGAAACTTTATGGATTGCTTCTAATCGTCTTCATCGACAAATTTTATCAATCTGACCTCGAATCAGGTGGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCACCTTTTGGTATTCCGAAAGGTATGCCTGTTTGAGTGTCTTGGAGAAACACATTCCTAAGGATTTGTTAATCCTTCCGAATGGACGTGGGAGTCGCCTTGATTCCAAGAGGCCCTCCTGAAATGCCTGAGTGGGAATTCCTATTGCACTCGCTGAACGTAATAAGTTTCGTTCGAGCAACTGCTAGGCCTGCTTACAACCCCAGGTTGCAATATACCTTTTAAGACTAGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCACGTGGTATTCCACGGGGCACGCCTGTTTGAGCGTCACGTTAACCATCGCGTCGTGGATGTAACAGTCTGCGGGGCGGATTTGGGTGTTGCCGTCGTTGGCTCGCCTCGAAATGCATTAGCTGTCTAAGGACACGCGCCGCTGGTGTGATAATTTCTTTGCGCCTATGGTGTTGTGACCGGTTGAAAGCTCATAATCGCTCGCAAGAGCAATTACTTTTAAGCATTGACCTCAAATCAGGTGGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACCTTCTAGTATTCTGGAGGGTATGCCTGTTCGAGCGTCATTACAACTATCAAGCACTGCTTGGTTTTGGATGTCACTTCTGTGGTGCATCTCAAAAGTATTGGCAGTAGCATCTAGCTTCTAGTGTAGTAAATTTCTTGCTATGGAGTTGGTGTTTAACTGTCAGAACCCCTAATATTTTATAAAGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACGTATTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCACTCCCTGGCATTCCGGGGAGTATGCCTGTTTCAGCATCATAAGCACCATCGAAAACCCCACCGAGAGGTGTGGATTTCGGACCTGGGCCTGCGAGGGGGGTGGTGGACAACAACAGCTCCCGCGCCGGCCTGAAATCGAGTCGCGTCGCGCAACCAGGTGGAGAGGAGGGGACGCGGGGGATCAAAACCTCGCTTTCCTCCGATCCCCAACGTCAAAGGCGTCATAGTTGGCCGCCCGTGGTCATCTTTCGCCGATGTTGGTGGTGGCGACGGAGGAACCAAACCCCATATCAAAGACTTGATCTGAAATCAGGCAGGATCACCCGCTGAACTTAA
## S1 1
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## GTGCGAAAAGTGATGCGATTTGCATGCTCTGTGAGTCATCGAATTTTTGAACGCATATGGCACCTGCTAGTTTTACTAGGAGGTATGCCTGTTTGAGAATCGTCTTTATCAATCCTCTTTTGATTCATTTCAGTTGAGGGACATGGGTTGTCATCAGTGACTGCCTTGAATATTCAATGTGGTTGTGTTGTGATATACTTGATCGACCAACTACCGTCATATTTCAGCGATCTCAAATCAGGCAGGATGACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTAAATTCTCAACCTTACCAGCTTTGCTGTTGGTTTGGCTTGGATGTGGGGGTATTCTGCAGGCTTTCACAAGTCAGCTCCCCTGAAATGTATTAGCGGTACCCTTGTGGTTAACGGCTACTGGTGTGATAATTATCTACGCCATTAGACGCCCACGTATATATGGGGGTTATGCTGCTTCTAACAGTCCATTGACTTGGACAAACTACAATGACAATTTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTTAGTGAATCATCGAATTTTTGAACGCATATTGCGCCTCTAGGTATTCCTTGAGGCATATCTGTTCGAGCGCCGTTTTAATACTATGGCTTTGCCTTGTGTTGATGGTGTTCTGTCGGGAACCCATTGAAAATGTCAAGTCGGCGCTGATTTTATGGTCTGATTCGCAGCAAAATGTGATGGACTTTTAATTAGTTGCCTTACTACTGAATTCATGGCCTCGGATCAGATAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ACGCGATAGTTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCATTGGTATTCCATTGGGCATGTCTGTTTGAGCGTCATTACAACCCTCGGTACCCACCGGTTTTGAGCGAGCTGCGGTTTCCGCCCGGCTGGCTTTAAAGTTGTAAGCTCTGCTGGCCGCCCGGCCCAACCAGAACATAGTAACATCATGCTTGTTCACGGTTCGCGGTCGAAGCGGTACGGCCTGAACAATACCTACCATTTCTCAAGGTTTGACCTCAGATCAGATAAGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCATTCGGCTTAGGGCACGTCTGCCTGGGCGTCACGCATCGCGTCGCTCCCCCACCCGTCCCTATGGGACGCTCGGTTGGCGGGGGCGGATAATGGCCTCCCGTGCGCAATGATGTGCGGCCGGCCCAAATTCGATCCCGCGGCGACTCACGTCACGACCAGTGGTGGTTGAACACTCGACTCTCGTGCTGTCGTGTCGGATTGCGTCGTCCGTCGGGTATTGTCGTAGACCCAATGGTGCGATTTTTTCGCGCTTCCGACCGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCAACTTGCGCTCTCTGGTATTCCGGAGAGCATGCCTGTTTGAGTGTCATGAAATCTCAACCATTAGGGTTTCTTAATGGCTTGGATTTGGGTGTTGCCAGTCTCTGGCTCGCCTTAAAGGAGTTAGCGAGTTTAACAATGTCGTCTGGCGTAATAAGTTTCGCTGGTAAGACTTGTGAAGTTTGCTTCTAATCGTCTTCGGACAATTACTTTGACTCTGGCCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAACGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCAAATTGCGCTTTCTGGTATTCCGGAGAGCATGCCTGTTTCAGAATCATTAAAACATCTGTTCTTGCAGGCCAGTGCACGCTGATATGAGGCGTGTGCTGTCCCGCAGAAGATGGGTCACTTAGTGGCGGTTTCTTCCGCCATTAACGATCCCGGAAATGATTGTGCGCGCCGGTCCTGTCCTGGCTGCTGATCAAAATGCAACAGAATATTCTCTATTTCATTTTGCGACGCAGTTTATAGGGCATTGGTCCGACGAGCGTACCCCCACCAATACAACTGGCCGTCAAAAAGCCAGAAACTTGGTCTGAAATCAGGCAAGAAAACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGTGATAAGTAATGCGAATTGCAGAATTCAGTGAGTCATCGAATCTTTGAACGCATATTGCGCCCTTTAGTATTCTGAAGGGCATGCCTGTCCGAGCGTCATTATCAACCATCAAGCTTGGCTTGCCGTTGGACCCTGCGCATGCTGGTAGTCAGCAATAGCAGGTCTTCAAGGTAGTGGCGGTGTCGTGGCGGACCCCGGATACAACGAGCTCTCTATAGCACGTATCAAGGTGGCCCCCCCGGTCCAGCCTCACTGGAATCTCACATCTAAAGGTTGACCTCGGATCAGGTAGGAGTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## GTGCGATAAGCAATGCGAATTGCAGAACCGTGAGTCATCAGATTTTTGAACGCAACTGGCGCTGGCTGGGTCTCCAGCCAGCATGCTTGTTTCAGTGTCTTGTTTTCTCCTCACCCAAAACTTAATGCGAGAGATACCCTTCTCTTGCCAAGCATGAAAGCACTCTTCTTCTGCGAGCGGCTCTTGACTTGTTCAAGGGTCGCACTCGATGCAGACGTCACATTTTACAATGTGAACTCATTGAGAGCAGAAGCAGTCAGCTAACGCTGTCAGTGCTTAGCCACTTAAACTTTATTTGCATCTGAAATCAAGCAGGATCACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCAAGGAGCATGCCTGTTTGAGTGTCATGAATTTTTCAGTCTTGTACACGTTATGTGTTTTAAGATTGGATAGTTGGGAGTGTTATGCCACGTTCACAAGAATGTTGGCTCTCCTGAAATTTATTGGAGTTTTGAACCCCTTTGTGGATCGGCCATTGGTGTGATAATTATCTACATCATGCTGTAAACGCGTATGGGGGTTTCTCCAGCTTAGCTTATTTGAAATCTTTGATGCTTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCTCCTCGGCATTCCGTGGAGCATGCCTGTTTGATGCGCATGAATAACTTCGGCCACTCGCGTGGCTTGTTTCTGAGCTTTCTCACTTTTGTGAGTAGTTCAAAGTCACAAAATTTTGAGAGGTCGCGATTGCTGGCAACGGCCCATTGGCTTAGTCCACCGGCGTCAAATGTTACACCTCCCAAGTGAACTGATAGTATACCACACCATCAGATAGCTTGGGACCATTTGATGATCAACCGAGGGACGGACATTTGGGTACGGACCTGCTCTCTGCTGATCTTTGTTGTGTGGGATCTTTCCCCTCAGAAATTCTGCATCAAATCAGGTAGGGTTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## TTGCGATATGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCATATTGCGCCCTCTGGTTATTCCAGAGGGTATGCCTGTTTGAGTGTCATTAATTTGTCAAACCAACCAACTTGTTTGATTGGTCTTGGATGTTGAGGGTTGCTGGATTTTTTTGATCAGCTCCCTTTAAATATATTAGCTTGGAACACATGAGCCACTTTAGCTTAGGCTGATATGAATATGTCTTGAATGCTTTTGCTGATGTGCTGCTTGTCTAGGCTTATAACTGTCCTATCTCAGGACTGCCTTTAGGCATCATCTTGATCATGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGATTTCCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCCTTGGTATTCCGAGGGGCATGCCTGTTTGAGTGTCATTAAACCATCAACCTCGGGGCTTTCGAGCCTTGGGGATTGGAGTTGGAGTGTGCTGGTGGAAACCCCCATCGGCTCCTCTCAAATGCATAAGCGGGAAACCTTTGCTTCCGAAAGTCGGTGTGATAAGGATTTGTTACGCCGTCGCATAGGAACTTTGAAGGCCGCTCACAATGGTCTTCGGACACGTCTATCAAACTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0
## ATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCCGGTACTCCGGGGGGCATACCTGTTCGAGCGCCGTTGTACTACTAAGGCTCAGCCTTGCGGTGGCCGCTTCCCCTCTTTGCAAAGAGAAGGGAACGGCTCAAACCAGTGGGCGTCTTTGTGAGGCTGTCCAGCGTAGCAAGTCTACTCGCTGAGCAGTTCCCCCCAGGGGCGCCAGTCAACGAACGTTTGGCCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
## S1 0
## S2 0
## S3 0
## S4 0
## S5 0
## S6 0dim(seqtab) ## [1] 74 551Nous obtenons 552 ASVs à partir des 74 000 séquences brutes que nous avions au début.
We get 552 ASVs from the 74 000 raw sequences we had at the beginning.
Inspectons la longueur de nos ASVs / Let’s inspect the ASVs’ lengths
plot(nchar(getSequences(seqtab))) 
Suppression des chimères / Remove chimeras
This The samples in the sequence table are all pooled together for bimera identification.
seqtab.nochim <- removeBimeraDenovo(seqtab,
method = "pooled",
multithread = TRUE,
verbose = TRUE) ## Identified 5 bimeras out of 551 input sequences.#save(seqtab.nochim, file="data/seqtab.nochim.rdata")Examinons les résultats / Let’s inspect the results
round((sum(seqtab.nochim)/sum(seqtab)*100),2) # Percentage of the total sequence reads## [1] 99.41plot(nchar(getSequences(seqtab.nochim))) # Lenght of the non-chimeric sequences
seqtab.nochim.bin <- ifelse(seqtab.nochim>0,1,0) Exclude singletons and doubletons prior to taxonomic assignment
seqtab.nochim.nosd = seqtab.nochim[,colSums(seqtab.nochim) > 200]
Assignation taxonomique / Taxonomy assignment
Nous voyons enfin le bout du pipeline avec cette importante étape d’assignation taxonomique. Grâce à l’implémentation de la méthode de classification naïve bayésienne, la fonction assignTaxonomy prend en entrée l’ensemble de séquences à classer, et un ensemble de séquences de référence avec une taxonomie connue pour émettre des assignations taxonomiques avec une confiance minimale de bootstrap spécifié avec le paramètre minBoot. La base de données de références (UNITE) est accessible sur ce lien https://unite.ut.ee/repository.php
We are finally going to the end of the pipeline with this important step of taxonomic assignment. Thanks to the implementation of the naïve Bayesian classification method, the ** assignTaxonomy ** function takes as input all the sequences to be classified, and a set of reference sequences with known taxonomy to issue taxonomic assignments with a minimum bootstrap confidence specified with the * minBoot * parameter. The database of references (UNITE) is accessible on this link https://unite.ut.ee/repository.php
getN <- function(x) sum(getUniques(x))
track <- cbind(out, # input & filtered
round(((out[,2]/out[,1])*100),2), # % (filtered / input)
sapply(mergers, getN), # Merged
round(((sapply(mergers, getN)/out[,1])*100),2), # % (Merged / Input)
rowSums(seqtab.nochim),# Non-chimeric
round(((rowSums(seqtab.nochim)/out[,1])*100),2),# % (Non-chimeric / Input)
rowSums(seqtab.nochim.bin)) # Number of ASVs per sample
colnames(track) <- c("Input",
"Filter",
"%Filt/In",
"Merge",
"%Mer/In",
"Non-chim",
"%Non-chim/In",
"#ASV/sample") # Column names
rownames(track) <- sample.names # Row names
track<-as.data.frame(track)
head(track)## Input Filter %Filt/In Merge %Mer/In Non-chim %Non-chim/In #ASV/sample
## S1 1000 595 59.5 576 57.6 576 57.6 68
## S2 1000 482 48.2 431 43.1 427 42.7 37
## S3 1000 556 55.6 481 48.1 481 48.1 30
## S4 1000 425 42.5 373 37.3 372 37.2 52
## S5 1000 387 38.7 328 32.8 327 32.7 59
## S6 1000 619 61.9 597 59.7 597 59.7 24{r assign_taxo, include=TRUE,cache=TRUE} #taxotab <- assignTaxonomy(seqtab.nochim, refFasta = "reference_database/sh_general_release_dynamic_01.12.2017.fasta", minBoot = 50, #Default 50. The minimum bootstrap confidence for assigning a taxonomic level. outputBootstraps = TRUE, multithread = TRUE) #
unique(unname(taxotab$tax[,7])) View(taxotab)